How to use Nanodrop to measure DNA/RNA

[@JaceTan-90]

Simple, straightforward and clear cut! Thank you!

[@kamaljitlall9329]

Absolutely marvellous video. Very concise, stepwise, explanation. Thank you.

[@MarceloSMota-vi8zd]

Very useful video! Thank you!

[@sanjit666]

Nice crisp demo

[@sara97979]

Great explanation, thank you 🙏🏻

[@HamdiAL-AZZANI]

Thank you so much dear brother

[@parvizabdullah]

Thanks a lot for your explain. It was very useful for me.Hope you'll share new videos.

[@innovative_learner]

You are welcome. Its a pleasure for me. Please let me know what kind of videos would you like me to post. Thanks.

[@jesse4828]

how do you get the raw data off the computer. all I can get is the report

[@sufiyan5095]

Thanks a lot for your explanation it's so useful Sir I have a doubt 3.29 what is that 50 below sample ID and beside DNA THANK YOU SIR

[@정성훈-n9r]

What are the principles of nanodrop machines? I know you're using the uv-vis method. How do you measure the dna concentration and wavelength?

[@SuleimanNasiru-ot3jy]

How can I use the values gotten to calculate the purity

[@innovative_learner]

Please consult the following link for detailed explanation: dna.uga.edu/wp-content/uploads/sites/51/2019/02/Note-on-the-260_280-and-260_230-Ratios.pdf

[@mohi7527]

Hi, thanks for the explanation. Did you add Milli-Q water or DE water for cleaning the lens?

[@innovative_learner]

Milli-Q water...thanks

[@hanyabdelgawadabdelsalamab7470]

Nice video, thanks for your help. Since you loaded 1.5 µl, is the result of the reading for 1.5 or for 1 µl ? ie the machine knows you loaded 1.5 and does the calculation automatically ?

[@innovative_learner]

It's not about the volume actually. The liquid fills the gap between the two surfaces. Moreover the volume used was consistently same. No, you don't need to enter volume in the programme.

[@bansisharma720]

What about concentration how to comment on the concentration we get at ug/ul

[@jerrysworld8242]

Same question. He didn't explain the concentration part

[@HamdiAL-AZZANI]

Thank you so much dear brother