What is a Standard Curve?

How much stuff is in that tube? This video will explain how to make and use a standard curve to measure the concentration of an unknown substance in s sample of blood / urine / mucus / whatever!
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[@YogurtHero]

In less than 7 minutes, you've explained everything in lecture I zoned out on, omg thank you!!

[@mariaorsic9763]

This is absolutely the BEST, MOST CLEAR, STRAIGHTFORWARD VIDEO I have ever had the pleasure of learning from! Thank you for making my day! College science should ALL be done with videos like this one

[@Penguinprof]

I'm so glad you found it useful!

[@maddoxzain920]

instablaster

[@ilikeyoghurt1]

Thank youuuu! I missed a biochemistry lab and I couldn't have done my practical exam without understanding this fully. Your explanations are so easy to follow. My lab book is covered in notes now :)

[@Penguinprof]

+Francesca Hewitt I'm SO HAPPY!!! Thanks for watching and letting me know!

[@tareqrf1693]

Kathryn Francesca what your University specialization?? I dont understand this curve in medical lab

[@ColleahG]

Oh thanks! Definitely helped me to understand for my biochemistry lab report!!

[@laurence4133]

Really good brush up lesson, cheers :D

[@asikurrahamanrahat5124]

Thank you so much for your detailed lecture.. I just loved it. It provides all basic answers of mine about this topic. Good luck 😊

[@citykeys3495]

Simple yet powerful explanation on stamdard curve and dealing with out of range samples.

[@Farvanoogy]

Excellent advice and methodology for making any measurement. Thanks!

[@Danielle-mu2rc]

Thank you so much! You did an amazing job of breaking it down...I've been in Molecular Biology class for a few weeks and during lab, the professor didn't bother to describe what a standard curve was. I was thinking, "where is the curve?!". I get it now, thanks to you.

[@wachowski9525]

I was going to wait for the video to at least get 1/3rd of the way before I was going to like it or not, but the Standard Curve definition and example with the consistently-themed penguin objects made me lol and informed, you deserve a like/sub!

[@Penguinprof]

I'm so glad to hear it - thanks so much!

[@rockmamabaker8370]

WOW! Thank you from the UK! My assignment is based on protein concentration in samples. This video explained it perfectly. I was clueless before I watched it! THANK YOU!!!!!

[@erickamendoza6418]

this was generally one of the best videos I've ever come across, thank you so much!

[@Penguinprof]

Thanks so much! Glad it was helpful.

[@karinamatos4253]

I will definitely subscribe. You are amazing explaining. Thank you so much.

[@Tiger-789]

Thank you so much, I had to use a standard curve for something in uni and I had no idea what I was doing haha This is so clear and I finally understand it!

[@martitka247]

Loved your explanation! Didn't have slightest idea in class what the standard curve is... Thank you!

[@nayudugaya3816]

Literally I watched and asked so many videos and people about this topic but still there are some lingering questions in mind. But this video cleared everything within 7 min. Tqsm

[@meenabarot2037]

Wow!!! no one can explain better than you!!! awesome presentation!!! Thank you so much!!

[@marouagares1757]

So clear and simple! thank you

[@evastamper]

I loved this - well done!

[@laynejones2100]

Really great video I didn't understand it in class and this video was so helpful and easy to understand. Thanks so much!!!

[@biorainbows4313]

Amazing! More videos like this please!

[@fatimahas7672]

thank you for your effort , its very simple and clear

[@59462Bella]

My online course is using this video as learning tool. Thanks :) Your video really helped.

[@sanalijayalath8096]

you're so kind i was desperately looking for this. thank you so soooo much!!!!

[@Penguinprof]

I'm so glad you found me! You are sooooo welcome!

[@fozomomo5734]

i love ur lectures!!! so helpful

[@kikiriki8742]

OMG, I've struggled my whole life to understand this and you just made it so easy and simple. Thank you!!

[@Penguinprof]

Yaaaassss!!!!!

[@rejeanhill4754]

6 years later and it's still a helpful vid , thanks miss u just helped me pass my medical interventions exam

[@Penguinprof]

SO happy to hear that! CONGRATULATIONS!!!

[@sunnysosa5117]

You are absolutely amazing!! God bless u!!

[@srishtisrivastava10]

I have been struggling to find the values watched so much stuff but youu explained it soo smoothly god bless uh🌈⭐

[@paigelewington1475]

fantastic video!!

[@afaq289]

thank you so much for this nice explanation. I really appreciate that!

[@user-hl9dr1ue9j]

Goddddd!!! This is exactly what i need, penguin prof ;D

[@loayk2754]

THE BEST AND CLEAR demonstration

[@Penguinprof]

Thank you so much! Glad it was helpful.

[@kamerus8892]

Fantastic and simple explanation for those of us who are assay beginners!

[@Suzuo73]

Fantastic video, I understood everything perfectly. The ruler example made it all click in my head, thank you so much.

[@Penguinprof]

You're very welcome! I'm so glad it clicked.

[@theeargasmchannel]

straight to the point thank you!

[@aliahjuarez1097]

Thank you, this is very informative

[@user-kt3xx5rq2x]

thank you so much for your video. really helpful, easy to understand.:)

[@hadeelalameen888]

thank you....easy to understand and very helpful

[@tolinavishalsharma1777]

Such clarity, that too in one shot🙏 wonderfully explained, Mam Thanks 🌸

[@Penguinprof]

So kind of you to say! Glad it was helpful!

[@MrTomswanie]

Excellent - clear, concise and educational! 2 questions, 1) How do you know what wavelength to use? 2) What is the difference between a spectrophotometer and a ELISA reader?

[@meredithwhitworth728]

Thank you! So easy to understand my assignment now! LIFE SAVING work done here today. Thank you Penguin Prof!

[@Penguinprof]

Glad it helped! You are most welcome!

[@tszyeungchu]

Or you can perform single/serial dilution, so that the unknown concentration falls within the 'ruler'?

[@puterimegat7]

AMAGAAAAAA THANK YOU SO MUCH for the longest time I felt so intellectually impaired but this video helped me loads

[@jancrisbustillo5512]

Thanks. It made it easier for me to understand it.

[@shaikhsahil8426]

Thank so much ma'am....that's topic make me so difficult to understand because already miss lectures by professor but now I totally got understood What exact standard curve is.....thank you again

[@Ashishjaj]

you cleared all my doubt in just 5 mins ..thank you

[@toastermax1]

You are the best!!! This is a really good contribution.

[@Penguinprof]

Thanks for watching!

[@Hoxgene]

Oh god. I needed that! Wonderful

[@Penguinprof]

SO glad you found it helpful!

[@ziliestarrive]

You really make it easy to understand, thanks a bunch.

[@Penguinprof]

+Po Ho You are so welcome - thanks for watching!

[@tanu02444]

Thank you, you explained very clearly.....

[@rm5700]

Nice and clear explanation! Thanks!

[@Penguinprof]

Glad it was helpful!

[@themindstorm9947]

How do you know which wavelength to use?

[@ClaudiaLopez-iy7rs]

Thanks you made it so much simpler than my professor did!!!!

[@misterpotato8965]

Thank you Profpenguin!

[@maggieedmunds9028]

Terrific, terrific...thank you.

[@anchisapoonprasartporn8232]

Thanks so much. You are brilliant!!

[@mustafahassan3316]

thank you so much doctor this video was so smooth to the brain

[@Penguinprof]

Smooth on the brain is a great compliment! Thanks!

[@sarahh.8026]

if I get an imperfect straight line, can it be used to find the concentration of the unknown solutions?

[@tareqaljazar2681]

Thank you so much

[@jessicawademusicJ19]

Very Helpful!

[@conceptbee1995]

I just fell in love with you. You just made it so easy. I spent all day trying to understand the graph of ELISA, but I was stuck on standard thing. Now I've got it thanks to you :D

[@Penguinprof]

I love you too! Glad it helped. You realize you're dating a penguin now. Just to be clear. :)

[@conceptbee1995]

+ThePenguinProf I'm so glad I am 😁

[@sabreeen_]

this was so freakn easily explained! ahh thank you so much

[@onlyjimmy4ever391]

Thanks maam , it really helpful , if the profs in our school can do the same lecture as u did , i think all of us will love to study in this course!!!

[@kellieashton3697]

Legend! Thank you! So easy to follow and understand....

[@hasnashah3600]

very well expalined.thank you.please make a video on TPC protocol and calculation

[@yashagrawal6525]

Thank you is the most that I can say tbh! what an amazing video!

[@Penguinprof]

Wow, thank you!

[@TheAcadianGuy]

couldn't understand when my teacher taught me, but I got it first try when you did, thank you!!!!!!!

[@thorshammer92odin3]

thanks very well explained, you must REALLY love penguins. haha

[@zellasayeed3657]

You are amazing!!!

[@sauldelossantos7924]

BIOTECH TEST TOMORROW AND I AM SO READY THANKS TO YOU!!!

[@Penguinprof]

+Saul De Los Santos So glad to hear it! GOOD LUCK!!!

[@TheCoolcaspar]

Great great video, so much easier to understand

[@Penguinprof]

Glad you think so!

[@chloeadams713]

In my biochemistry unit we were told that if the absorbance is higher than the standard curve reads that we should dilute the unknown and then use the dilution factor to find the initial concentration. Is that not a solution to that problem, or can you do either?

[@israakareem5304]

Thanks a lot this is so helpful

[@jermanijeane8775]

Thank you so much! I really needed this clarity!!!!

[@Penguinprof]

You are so welcome! Thanks for watching!

[@nicmaanqaadun9289]

Thanks you so much that helped so much.

[@sarazahid827]

Excellent video, i had made lot of mistake and extended the x axis and y axis . but you have discussed it very clearly.thank u so much

[@Penguinprof]

Glad it helped!

[@TheRdChrry]

this is amazing! thank you so much

[@Penguinprof]

You're so welcome!

[@hibbaabdilrazak4230]

Your video is amazing.

[@Penguinprof]

Thank you! Glad it was helpful.

[@Hoalefstvn]

Tks for share. You rock!

[@sageofinfinitepaths5738]

Thank you so much from Africa, i just subscribed. That biochem lab won't know what hit it.

[@abbyjutilo7638]

very helpful and very cute penguins!! thanks so much!

[@Penguinprof]

+Abby Jutilo You are so welcome! Penguins make everything easier, right?

[@ilbelviso]

This is very clear, thank you.

[@Penguinprof]

Glad it was helpful!

[@andreas.griffin1328]

Nice video, thank you. I have found a published standard curve for lutein (a carotenoid) (openi.nlm.nih.gov/detailedresult.php?img=PMC4206223_f02_00&req=4) but it says it is a chromatogram standard curve; can I use this for a spectrophotometric analysis of lutein concentration?

[@nickchan8592]

You've just made my life a bit easier. thank you

[@Penguinprof]

Woo hoo!!! Thanks for letting me know!

[@juliuschen6551]

Thank You So Much!!!!

[@farahloqman5874]

Thank you so much!

[@mrsbakhriba7753]

u are amaaaazing thank you

[@solakhalid8967]

I have question.. Can you solution by regression line equation if absorbance more than 1.4?

[@Levepalestine11]

It helped me a lot. Thanks 🙏

[@Penguinprof]

Glad it helped!

[@arsonaleleth4877]

Omg thank you so much for this knowledge you saved my academic life

[@Penguinprof]

Wow!!! You are so welcome!

[@vickismith8533]

Ahhhh. I understand this now. I didn't in my class. Thank you very much.

[@Learnwithsimon27]

Woww..this is just wonderful.

[@havvaunal3957]

wonderful explanation,

[@Penguinprof]

So glad it was helpful!

[@FootyShorts510]

Very helpful! Thank you so much!

[@Penguinprof]

You're very welcome!

[@pumpkincal4411]

Thank you so much!!

[@NaushadKhan-ix2ue]

amazing! thank you

[@joymureithi981]

Made that look easy ☺️

[@cy4n_knight_q8]

Tbh.. this is the best video for explaining this.. finally I understood what's going on

[@Penguinprof]

I'm so happy to hear that! Thanks for letting me know!

[@marialaish8253]

How to make the higher concentration of maltose standard curve, my values are not falling the standard curve. I take 0.2, 0.4, 0.6, 0.8 and 1ml of standard maltose and make up the volume to 2ml with distilled water