I Grew Real Spider Silk Using Yeast

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What started as a dream more than 10 years ago, has finally become reality. After more than 2 years of work, dozen of failures, hundreds of hours of lab work and design time, we've finally done it. We've engineered a strain of yeast that produce real spider silk! This video explains how.
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Previous videos:
Project overview - • Spider Beer - Making Y...
DNA extraction - • Extracting Spider/Bact...
PCR - • Everything You Could W...
Journey to the Microcosmos - / @journeytomicro
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[@whatdamath]

dude...

[@outofcontext728]

X2

[@Krakburg]

love your videos

[@chickenpotpieare3things]

Anton came out of the Solar System to check out this amazingness!!!

[@GrantH2606]

hello wonderful person

[@pyrodamix4771]

LOL

[@cg2229]

me laying in bed eating cheetos: ah yes of course biomineralization peptides

[@ghostlydude6478]

This is basically me trying to understand how kars survived falling into lava, but with *Spiders*

[@aog1747]

Hell yea😂😂😂

[@xephyre6955]

I dont even understand algebra let alone this. But the idea is interesting enough for me.

[@v1298]

I saw a post online about the iron snail a couple of days ago, so I'm basically an expert

[@saki1333]

@@v1298 yes xD

[@zebbofrags494]

Sad to see this experiment hasn’t been taken any further in 3 years. This genuinely made me feel interested in science for the first time in my short 20 year life.

[@thethoughtemporium]

It's being worked on, but we're trying to commercialize it so can't share updates yet

[@_dylansaeed]

@@thethoughtemporiumthanks, I was wondering why it wasn’t being continued

[@sasukekun1997]

This had made so damn happy. I thought this project was long forgotten

[@Delapadation]

​​@@thethoughtemporiumI would think that this would get funding fairly easily due to how strong spider silk is and the amazing utility we could get out of growable high strength building materials, and something I just thought of while watching the video explaining the structure, spider silk is a fabricy version of reinforced concrete.

[@Yogaismehh]

@@thethoughtemporiumlike a suburban superhero???

[@joelswartzentruber9561]

Instead of wet spinning, try electrospinning. It should give you aligned fibers instead of randomly oriented ones from wet spinning. You should be able to cut a section and twist the web fibers into actual thread that are theoretically aligned so you get better load sharing on each fiber.

[@no_name4485]

mad scientists in movies: I will take over the world mad scientists in real life: I will make beer out of spiders

[@greenaum]

Technically making spiders out of beer.

[@tartanhandbag]

@@greenaum technically making something not very beer-like out of a yeast variant with spider DNA spliced into it

[@Butter-Milk]

We have more realistic goals, then?

[@morro570]

@@Butter-Milk who’s we you aren’t a mad scientist

[@what908]

mado scientisto in animes: El Psy Congroo.

[@user-N20]

No wonder Tony Stark looked so impressed when Peter said he manufactured his webbing all by himself.

[@bladeoftheruinedking2543]

Yes

[@nams0ldier584]

Yes

[@cringepuppetguy]

Yes

[@duskycyberian7257]

Yes

[@jonathanshepherd6423]

Yes

[@sparking023]

I have absolutely zero biochemistry background, but I somehow feel like I understood most of what you explained in this here video. Amazing work, and as a Spider-Man fan, I'm eager to know more about the possibilities of IRL webshooters

[@Noflippingwaydude]

Don’t worry once I have money I’ll make them

[@the_undead]

This part you probably already knew, but using IRL web shooters with absolutely be one of the most dangerous things you've ever done or did anyone could realistically ever do, but spider silk binded with carbon nano tubes. Could absolutely if made correctly hold the weight of multiple humans, I don't think you're doing Oh that the web shooter could do in a little canister. That's the size of your pinky but in especially designed flat pack tank that goes over your stomach, now we're talking. Because of how easy it would be to vary the properties of the silk when you produce it, depending on which particular strand of genes you use, everything you see Spider-Man doing with webs would be at least theoretically possible to do with at least a version of what is demonstrated in this video

[@ShDw-cc1bs]

So what is the current status of this project? Have you scaled up the process and/or have enough of a sample to play around with for a video demonstration? Would be incredibly interested in obtaining a sample of the yeast for a related personal project (involving kombucha SCOBY bacteria) if at all possible.

[@JoelCreates]

I'll be following the development closely since, you know, I'm something of a scientist myself...

[@kenzima6934]

Lmao when is the Hot Glue Web shooter swinging video coming up?

[@ivoryas1696]

Joel Creates I wanna say same, _but..._ I don't know calculus yet (among many _many_ other things that would likely leave me a bumbling fool in most any *_real_* lab) 😞.

[@JoelCreates]

@@ivoryas1696 I was referencing the Spider-Man meme, since I recently built a hot glue web shooter. Technically I'm a musician...

[@ivoryas1696]

@Joel Creates Neato! I'll check it out. -Also, I'm somewhat of a musician myself!-

[@norlavine]

Me too - my latest project is observing the time it takes to cook a '3 minute egg'.

[@davieuacho]

Me, not understanding anything but still enjoying it none the less: "Ah, shit, they can't print repetitive sequences, what a bummer dude"

[@evangrimm1702]

😭

[@hardanalljr.3138]

Yup I'm a total Chad ,don't understand shit but it's cool

[@liviawong6928]

@@hardanalljr.3138 Are you build as a Chad as well? Cause, cool.

[@twistedjosh14]

I honestly didn't understand what you were talking about. But I was intrigued the entire time. I didn't even know it was possible to PRINT DNA. This is some crazy stuff

[@darkestwilightt]

23:42 "Thing number one.... be gentle with the yeast...." I just love the way he said that

[@lukasd.4389]

"I've become quite competent at designing dna from scratch" Why does this sound so menacing. But dude, youre awesome

[@0Clewi0]

because of prions

[@hunterbakko9306]

I mean he's kinda playing God so yes it is menacing

[@adventurer3288]

You WHAT

[@superchinmayplays]

i can now become spiderman

[@wobblewokgaming655]

*He's becoming a Spider-Man villain*

[@jacob2802]

Dude, I cant believe you're making this technology open source. I've been reading about the possibilities of harnessing spider silk since I was a dork in middle school, and remember thinking the guys making the spider goats were cool as hell. I really hope you manage to extract and polymerize silk from this.

[@aserta]

Because he's one of the good guys, not subverting science for the highest bidder. He's one of those scientists that need crowdfunding the most, to keep him free of the likes of bigCorpo who'd put a life patent latch on it and shut it for good.

[@davidbergmann8948]

Haha yeah it's awesome, right? 🍄

[@rihardsrozans6920]

Right? I remember watching Ripley's believe it or not as a child and one of the guys featured there had made a really durable, strong suit out of bunch of materials including spider silk he milked out of actual spiders and since then I wondered about the possibilities of creating some super hero costume out of spider silk. It's truly wonderful that people are coming together to make education easily accesible and likely creating future scientists.

[@garethbaus5471]

Same here.

[@suhanrahaman4342]

Even though spider sill is a real good material it also get most of its strength through how the spider twists the silk together

[@Jeff-ti4pd]

Man im so happy for you! I watched the first video a couple years ago and I was very anticipating the final and finished product! Congratulations on figuring it out and getting it to work!

[@regulargreg]

I had a professor in college. This is the kind of project he was barking about in his often encouraged tangents during biopolymer class. Your project is like a customized and detailed exploration that goes well beyond what he could elaborate on in class. Thank you for peaking my interest!

[@AppliedScience]

Wow, what an incredible project! Your dedication and perseverance is an inspiration!

[@thethoughtemporium]

Thank you so much! Once I've got some fibers made, I'd be happy to send them over for you to play around with if you'd like

[@KnowBigDeal]

Here's an idea: Electron Microscopy videos of all the mineralised silks!

[@simonRTJ]

@@thethoughtemporium Maybe Ben could X-ray diffract image them, or run them through a mass-spec, or the Electron mic, or ALL THREE! amazing work, there should be a "KZbin science medal" or something.

[@MrEtronic]

i think we are on the cusp of a revolution ... who needs elsevier , springer or iEEE these dudes together with a couple other sci-tubers are doing more cutting edge research than many well funded snob colleges.

[@Srfingfreak]

@@thethoughtemporium Growing fibers is cool - But I would reccoment that you try mixing some into a Kombucha brew and make a SCOBY - you'll get nanocellulose tied to fibroin... it might be revolutionarily strong. Use weak/white tea if possible, and you can actually just use mineral supplements in sugar water for a "cleaner" SCOBY. Also, that way, the yeast/bacteria make the sheet for you, and you don't have to worry about the fiber weaving process.

[@Nighthawkinlight]

Instant thumbs up. Been waiting for this one.

[@-NGC-6302-]

Me too. Great videos from the both of you.

[@jonapple2325]

Are you okay

[@JKTCGMV13]

This comment prompted me to give the video an instant thumbs up

[@PakleniVuk]

I will combine Yeast and Starlite and rule the World!

[@stiepanholkien605]

Now he just needs little wrist siphons.

[@JeskaDax]

Been following your progress on this for so long. It's awesome to hear you're over that major hurdle! I look forward to hearing how the rest of your experiments go!

[@patrickhayes3099]

Glad to see this! 40 years ago I walked through a black widow web and began wondering about how to harvest the silk to make fabric.

[@tatianatub]

"the plasmid has been shared on my github repo for that want to mess around with it" imagine saying that sentence to someone in genetic engineering 25 years ago

[@otheraccount5252]

well, we didn't have git back then

[@ArnoldsKtm]

@@otheraccount5252 NO SHIT SHERLOCK

[@recklesflam1ngo968]

@@otheraccount5252 wow really

[@Josuh]

@@otheraccount5252 Really???? I thought github was like hundreds of years old omg thank you

[@theawsomnessdude]

@@Josuh the whacky part is that this sentence might someday be true.

[@Shotgun_Gospel]

Guy who invented penicillin: "Patent? That's absurd. You wouldn't patent the sun, would you?" Biotech firms: "Spider silk? Oh that was us, we invented that. Ours".

[@sontapaa11jokulainen94]

Capitalism go brrrrr

[@Hoshimaru57]

If someone could patent the sun they would

[@nucleartoaster01]

Let's not encourage them

[@nzoomed]

@@Hoshimaru57 its called nuclear fusion!

[@nzoomed]

Can someone explain how anyone could hold a patent to a gene found in spiders to begin with?

[@ohboy1113]

This and the making opal video make this the best channel I’ve seen, hands down. Easiest sub of my life.

[@lancewalker2595]

This is by far the most incredible, most inspiring thing I've ever seen. People like you are the future, I truly believe that. Unbelievable.

[@jadegreenleaf781]

Arachnologists for decades: "If only we could produce spider silk" Thought Emporium: "hold my spider beer."

[@TheWretchedOwl]

Now I want a “hold my spider beer” shirt.

[@omniscientbarebones]

Hold my Southern Black-Widow in Ethanol*

[@ultrachad289]

Ｏ－Ｏ　ＷＨＡＴ　ＴＨＥ　ＦＵＣＫ

[@alba2162]

I wonder what spider beer taste like

[@trajectoryunown]

Spider Cider - Made with real spiders

[@MagnusDangerMagnus]

30:15 I love that I live in the future. The idea that I share the world with a KZbin-vectored, open-source, melt-printable, github-published bio-kevlar makes me happier as an adult than ice-cream did as a child. Thank you.

[@makarismaileadnan]

I really don't get what you're saying, but I'm happy that someone's happy in this world

[@gavart4509]

Makar Ismaile Adnan He’s happy that the creator of this video is so transparent with his process and so willing to share his results. Plus, the fact that this content is distributed for free.

[@pacificxplorer]

This is really “the future” it’s the transitional period between the past and the future. Right now everything cutting edge is being discovered and all these cool products will most likely be widely available by the time we’re all dead or really old.

[@samuelr.6046]

Is it just me or is it crazy that they can Patten certain animal genes, that's absolutely some dystopian stuff right there.

[@harrisoncord3680]

make a web shooter and only if the silk was strong enough, you could be spiderman.

[@xomvoid_akaluchiru_987]

This is incredible!! I've never been able to keep up on all the scientific news and now I can catch up on some bits I've been interested in for years now :)

[@crackedemerald4930]

*shakes yeast vigorously* "they're a bit of a diva ngl"

[@nicholassoultanian5455]

Someone give this guy a like a giant research fund. he deserves it.

[@diamond4xe734]

IKR dis crap is insane

[@minermortal1997]

Where’s Oscorp when you need’em

[@EmmaHopman]

Patreon

[@fuckdeadfaces]

@@theunknowngamer5477 You know those movies are fake right lol

[@fleurgymcheurgy9267]

@@fuckdeadfaces for now...

[@AceKaci]

I am beyond impressed by this (well, all of your videos really). Can’t wait to see where this project goes next.

[@FSantora]

I can't believe you were able to explain this in a way I almost completely understand! I learned SO MUCH from this!

[@FSantora]

I would be interested in where LOTUS silk falls on the graph at 10:10

[@ElderSign32]

it's insane that this is even possible. i feel like a caveman listening to this

[@vappyenjoyer24]

advanced homeman

[@wataruishihara1570]

I’m just sitting here wondering how Peter Parker did all of this

[@BRONZETOAST]

I'm wondering why I've been searching for a Spiderman comment. Thank you

[@lordofhnajuty]

Same as tony, with a box of scraps but in a slightly nicer apartment.

[@brdiejustvibin143]

did it in high school too ..,like how

[@Loice_0]

The excuse is that Peter feels the necessity of making spider silk so that’s why he can make it? It’s really weird if I’m honest

[@gavinhicks7621]

Well Toby McGuire his webs were natural Andrew Garfields were stolen from oscorp and Tom Holland I don’t know but I’m pretty sure in the comics (I’ve never read them so this might be wrong) the spider bite gave him the formula composition and he was able to buy the products to make it

[@tacotaquero1680]

I loved this video, i really hope you keep on this project, it really seems to have a great future

[@trkoo]

What happened to this project? Can we get an update?

[@reedbrooks3887]

Spiders: You will never be able to farm our silk... Humans: Ok, we’ll just steal your genes and put them in other organisms.

[@Salt_discriminater]

Hippity hoppity your genes are my property

[@nightelfmohawk9821]

Surely nothing can go wrong when mankind tampers with nature... GMO is scary stuff

[@fred_e]

@@nightelfmohawk9821 Well, I mean, spiders are scary too, I guess.

[@fred_e]

@@nightelfmohawk9821 And chihuahuas

[@lolgamez9171]

@@nightelfmohawk9821 k but, how is farming spider silk dangerous

[@kastiak06]

"New merch coming soon! Who wants some homegrown T-shirt?!"

[@billeethesciencegeek]

"I love it so much, I can't seem to take it off!"

[@GuilhermeCargnelutti]

The yellow ones are edible, but don't try the blue ones

[@Scott_C]

Only $9,999.00. 😄🤑

[@pablozurita2996]

@@GuilhermeCargnelutti I'm gonna eat the blue one

[@tryplot]

the colors wouldn't even need to be dyed. they could actually be from the silk it's self

[@samdwichenjoyer]

Rewatching this video a second time and chuckled thinking about how people used to think everything was made of air, water, fire, and earth, and here you are doing this sorcery

[@Leatherargento]

There's I love your channel. I'm in my late 40s, and I'm still surprised, and often, by your content.

[@TechIngredients]

As fellow You Tubers we continue to try to improve our content aware of the friendly "competition". You keep raising the bar...Damn Excellent video, keep it up!

[@thethoughtemporium]

Thanks!!

[@eatonkuntz]

hey Tech Ingredients I love your channel too

[@VengeanceCore]

@@communist754 lol

[@spambotbro839]

@@thethoughtemporium You should collab with NileRed

[@bmxscape]

@@communist754 why would he collab with someone who no one knows xD he doesnt even know if you know anything about the topic

[@ViktorVano]

I am a software developer, but I am totally amazed how advanced and well understood biochemistry is.

[@instantnoodles8640]

I am a teenager, but I am totally amazed how advanced and well understood biochemistry is.

[@themblue8236]

I am an idiot, but I am totally amazed how advanced and well understood biochemistry is.

[@philcollinslover56705]

@@themblue8236 lmaoo

[@mastershooter64]

I am a biomolecule, but I am totally amazed how advanced and well understood I am

[@johndawson6057]

@@mastershooter64 you win this thread

[@kalvincochran9505]

I love that I subscribed to this channel probably about 2 month ago idk, and then I look up in my KZbin search bar synthetic spider silk bc I had seen it in a paper but I was so thrilled to see you had a video on it! Love your content keep it up! Also sick earrings bro hahaha!

[@kairon156]

This is so cool. While I'm no scientist I really enjoy hearing about material science and other such things we can make through detailed processes.

[@TheMysticGauntlet]

Peter Parker : No one can figure out my super secret spider web recipe. The Thought Emporium : Hold my beer.

[@uxleumas]

isn't all beer made out of yeast though?

[@bovanshi6564]

Hold my spider beer*

[@pete8420]

Hold my test tubes

[@TheMysticGauntlet]

@@bovanshi6564 Spiderbeer Spiderbeer... Does everything a spider can... Make a web? Anytime... Look out... Here comes the Spiderbeer...

[@SquaredSmith]

Hold my beer but don't drink it. It's full of spider silk and tastes terrible.

[@kentvandervelden]

Pretty cool, and there's more power in presenting on KZbin instead at academic conferences.

[@Barskor1]

But my tenure!

[@philanthropicnightmare1206]

A lot more. Get ready for an influx of scientists making KZbin channels.

[@icdeamons112]

'With great power, comes great responsibility.

[@catwithagun902]

@@blackjoker2345 REALLY great profit margins

[@konradandrag845]

I’m 15 and I don’t understand most of this but this has inspired me to learn more about biology and also I’ve designed my own web shooters if I could find a way to get the silk liquid.

[@gxrimess]

Dude. This is awesome. You earned a new sub dude.

[@Hlebuw3k]

"I was able to purchase a whole vile of nightmare fuel for a grand total of 15 dollars" I did not need to know the price of an arachnophob's sanity.

[@minermortal1997]

Secret Santa gone hardcore

[@knctvrknctvr]

@@minermortal1997 now THATS what I call an office party!

[@legendarysideburns2213]

Next project: yeast that makes spiders

[@sleezymechanic]

Next next project: spiders that make beer

[@notajalapeno4442]

@@sleezymechanic next next next project:yeast that makes dimonds

[@cg_pizza]

@@notajalapeno4442 next next next next project: yeast that make any material

[@mooneymakes359]

next project: watch youtube and feel like I have done something

[@mooneymakes359]

next project: clean kitchen

[@twosongs7396]

I was just speaking about mixing spider silk with graphene on Twitter this morning. You’re bang on! Kudos! I thought this new form of silk could be used to encapsulate and drag an incoming asteroid. You may have saved the world or least, you just might, if you continue.

[@Minkomans]

Massive respect to people that do this sorta stuff, I look at this like wtf is going on. I don't have the brains to get to this type of level of knowledge. keep it up dude!!

[@TacetCat]

My mind is blown. I knew bioengineering was a field that existed, but had never *really* thought about it. When I watch a video on chemistry or physics, I usually get most of it. I understand enough about chemistry to usually follow along with whatever video I'm watching, even if it goes to the slightly more technical side of things. *This* video was 33 minutes of me going "wat." Okay, making yeast produce things. I've heard of that. Wait, you can just write DNA like you're a programmer? Wait, there are companies that will print DNA projects for you? This is common enough that a business can be made for it? Wait, you're taking a bunch of different genes and randomizing them and somehow you get the properties you want? Oh, silk sort of doubles back on itself for added strength; I get th- Wait, you can just add properties to it like it's a new spell in a video game? Wait... Suffice it to say, I think the remainder of my day is going to be spent learning as much as I can about this. Fascinating is too weak a word.

[@neut1121]

that's exactly what was going on in my mind watching this video

[@HELL0RWIN]

Yep. I feel totally the same. I want to know more.

[@TheMightyZwom]

Same here... just... wow...

[@nikkoa.3639]

You've found your resolve my friend.

[@Someone-ig7we]

IKR it's like a game xD. You can just add whatever you want??? Lmao sign me up!

[@transformerslov]

Its so weird. He makes me feel like I can understand everything he is saying but at the same time I'm like 👁👄👁 okay 👍

[@mechadonia]

4 mins in “what is he even talking about” 20 mins in “of course the pPCI9k needs to be linearized with a restriction enzyme! You can’t have those DNA rings, duh!!!”

[@auve0G]

it sounds like foreign language

[@nateracing]

I interpreted this as an informal video-essay type of report on his findings, truly fascinating, yet extremely confusing to people without bio-majors, like me ;)

[@ApolloLax]

This dude has taught me so much! I would love to get into biotechnology and this guy inspires me!

[@lucyhalut4028]

Looking forward to the follow up :) Great Work!

[@Gennys]

Someone needs to explain to me how any natural, unmodified living organism can have patents put on their genes in the first place...

[@btw1095]

Brian thats what we get in a a country run by corporations

[@ahtheh]

Huh I've been learning that sht to get marks from the 9th Grade and now I can use that knowledge to brag to a random guy on the internet because I know it and he doesn't [I now realize that's all that information is every going to be useful for]

[@senjugold6977]

Lmao imagine existing and the big companies are like: "your genes are mine!"

[@_ThistL_]

Kinda reminds me of the tree that legally owns both itself and the land around it.

[@onidaaitsubasa4177]

I know right, it seems that there would be some legal trouble with whoever made the patent and the original genetic designer of the animals, or would the actual spider be the patent holder of the silk since it originally created it, either way it doesn't exactly seem legal.

[@crookeddesk]

"I've become quite competent at designing DNA from scratch" "and just have a company PRINT the DNA" Jesus christ watching your videos always makes me awe at the times we live in...

[@kingbroseph9773]

wait till he can print his own DNA without a company

[@TheGoldenCulture]

bro this was a year ago we need an update. i need more. also this was the first video i watched of you and subbed just cause of it.

[@forsomereason3713]

I have no knowledge in biology but you're such a great teacher I'm amazed I'm understanding (almost) everything and keep binging your channel with an unquenchable curiosity for knowledge now! This is the best mental pr0n I've ever been addicted to so far.

[@Palitato]

"I couldn't isolate the DNA, so I just made it myself."

[@davidmella1174]

...

[@aferrone92]

I literally lost it when he said that

[@ProctasisLimerna]

I will make my own DNA with hookers and blackjack

[@Lucas-iSL]

@@ProctasisLimerna and without the N.A.T.O!

[@minermortal1997]

@@ProctasisLimerna this sounds like a Cave Johnson quote.

[@KiJ0_0]

Spider-Man’s web fluid is now becoming a reality

[@zmalque9438]

FAVIAX Delatorre hol up

[@alejandrobernal4987]

Indeed it is, my friend

[@Josuh]

@FAVIAX Delatorre 😤😤😤ಠ‿ಠ

[@nikkiofthevalley]

@FAVIAX Delatorre That's f**ked up.

[@KiJ0_0]

FAVIAX Delatorre get out.

[@AdenijiMathew]

This is such an amazing content with great discovery and it is explained in detail that a complete novice can easily comprehend the content. great

[@mitchellcuthbertson1212]

This is the most incredible thing I've ever seen

[@booker...]

Haha that spiders pretty hot amiright guys

[@suicideiscla55ic]

Can't wait for the video on "spidey-sense".

[@0th_Law]

@@suicideiscla55ic The real question: how would you go about achieving it?

[@bleachisgoodforu9341]

Get bit by every spider

[@LOOTS243]

Spiderboiii

[@ViktorTheMusician]

@@0th_Law Tarantula DNA? They use their hair to detect changes in the air. Spidey Sense was meant more to represent an invisible web of sorts, though I've also seen it as representing the 8 eyes spiders have though they actually have really bad eyesight. But by coincidence Tarantulas already have a spidey sense going for them.

[@Ryan6.022]

Spidey yeast Spidey yeast Produces proteins a spider can Makes a fiber any size, matches kevlar any time Look out here comes the spider yeast Is it strong? Listen bud,he's got enhanced gene strands. Can it be made into a thread? Take a look over head. Hey there goes the spider yeast!

[@Froyorionpop]

Nice

[@BrennonIsMilak]

I don't quite understand but I am fascinated. It's especially interesting how complex making silks is for us and spiders just.. do it.

[@rhyskendall8379]

Gl mate! I will be along for the ride and be here when you accomplish it!

[@pvc988]

"First, its silk gene wasn't protected by any patents…" Wait, WUT?

[@Kharmitas]

The first entity to isolate a particular gene gets to file a patent on it under the current biotech regs. Leads to some very undesirable possibilities. It's one reason to be suspicious of those 'we'll analyze your DNA to determine your ancestry/risks' things, because it's potentially a mechanism for someone to gain a monopoly on all commercial use of one of your genes for the next few decades.

[@wacesferpit]

capitalism

[@Kharmitas]

@@wacesferpit That's the more succinct explanation yeah.

[@eichenbrain6170]

@@Kharmitas ... and then (after the 20 years) your own personal gene is open to the public to use as they see fit.

[@Kharmitas]

@@eichenbrain6170 Twenty years after a corporation had the opportunity to raise the price of whatever treatment it may have turned out to be useful for to the point that thousands of potential beneficiaries went past the point of no return without ever having the capability to pay for the treatment.

[@Z31HoodMoments]

“Was able to purchase a whole vile of nightmare fuel”

[@nikkiofthevalley]

Lol, he's not wrong.

[@huntercool2232]

“I grew real spider silk using yeast!” *Peter Parker wants to know your location*

[@KanalBiyogen]

My heart stopped at the biomineralization part when I saw the diatoms. I miss phytoliths 😢 Also such great syntethic biology project I'm in great awe

[@ShamelessDuck]

I can't imagine I live in the time when THIS is possible. You are in my "God-Tier KZbinrs" list

[@tormodhag6824]

ShamelessDuck like you can print DNA?! that is kinda crazy, just humans writing their very own DNA designed specifically to hijack in this example yeast. I searched DNA printing on google and you can apparently make custom creatures with it, which is insane

[@ShamelessDuck]

@@tormodhag6824 I dream of future when we will use GMOs legally in our everyday life without it being marketed as "non-GMO" and without this whole stigma around it.

[@puppypi9668]

Ikr And I love how the God-Tier KZbinrs all know each other XD Applied-"I built a scanning electron microscope from scratch in my shop"-Science and Tech-"I made a nanostructure imprinted chocolate bar diffraction grating"-Ingredients both commented here alongside us! XD

[@user-N20]

All the people who want to be Spiderman: *_confused but encouraged_* "Write that down! Write that down!"

[@jameskelly9277]

Lmaooo why would you call me out like this!!

[@nightastheold9094]

Lol I was in 7th grade and the 2002 Spider-Man came out. My friend and I thought we could make web shooters from aluminum cans and webbing from kitchen spices and cleaners.

[@alionicle]

Aight, that's me right now

[@demoninfested._.nugget5675]

I want to make a web shooter

[@kete6640]

Ive been tryna figuring out how to be spider man for the past 3 yrs lol, and I DIDN’T RUN A SINGLE TEST BECAUSE 12 YR OLDS CAN’T GET A HOLD OF NYLON 6, 10 SILICONE AND ADHESIVE GEL. I ALSO WANTED TO ADD HIS SPIDER SILK LIQUID THING BUT I’M TO “UN-INTELLIGENT” TO MAKE IT. AND DON’T ASK WHY I’M YELLING BECAUSE IDK

[@zachbanks152]

THIS GUY GIVES ME A NEW RESPECT FOR THE INERWORKINGS OF BIOLOGICAL PROCESSES

[@BeaChapman]

I still can't believe people can write DNA in Google docs. I hope to do that someday.

[@StudioSkiesAndWater]

Remember what he said tho

[@Deltazulu2000]

"A whole vial of nightmare fuel" I've never heard that be so damn accurate

[@BahhBahhBrownSheep]

I’m pretty sure that’s a statement you’ve never heard before, or in the two years since. So that’s not particularly impressive

[@passingrando6457]

​@@BahhBahhBrownSheepCounterpoint: the Pony Jar Project exists.

[@ziggiey]

All i can imagine is him looking at the genetic code and going "mmmm, silky"

[@fionafiona1146]

so smooth

[@vasudevraghav2109]

There's something silky going on......

[@jakeholland6194]

This is truly amazing

[@eztoast1179]

Me after watching this video: *"You know, I'm something of a scientist myself."*

[@baldiesss]

Type of guy to clone himself and go on vacation and we won't notice

[@Snailcrossing]

KRIEGER!

[@natorious314]

I can't express how much I love that you document all this content for us to watch. Science in all fields can be so interesting, but it's so insular and stuck in the world of papers and journals that the average citizen will never hear about all the cool shit going on. You and many other science youtubers are really doing a great service.

[@3asianassassin]

It's often that insularity that convinces people to take up and believe pseudoscience and eventually erode trust in science and data

[@tbs0000]

You call fax I mean we are all forced so deep into the thought that science is all boring stuff on paper but no it's fun aswell like artificial meat or aerogel and theories like our universe isn't finite and it's a loop from one point to another or that aerogel can be used in rockets as insulators.

[@assfuckerthejointpounder5834]

I remember how in the action lab they mentioned that the structural strength of steel regarding tensile strength is in the gigapascals. Even specialized steel it's not like it's 10,000 times which spider silk can handle or whatever. Who knows how many creatures have a shell made out of nacre oh, it's tensile strength depending on the species is 170 gigapascals, even if one gigapascal is 2 megapascals this is still a whole lot more than steel. The trouble is this bad boy takes 170 gigapascals to bend, or is that bend it to a point that it will no longer Bend back. Either way that is a whole lot. I did the conversion for both of those, 170 gigapascals is more than 24 million pounds per square inch, and 70 is more than ten million. I did the conversion for both, I checked the conversion multiple times for the tensile strength. I'm pretty sure that I did the 70 kg pascals correctly given how much of it was copying and pasting, and using an online calculator. This is far beyond Steel. Now this is so strong because of the way that it is aligned, according to Google it's like more than 300 or something times stronger than the material it's made out of. So imagine graphene was in the structure that this material is in. And I'm not talking about the structure of which its atoms are aligned. This bad boy is made in a structure of pretty much interlocking tiles, so imagine this was what was going on with a flat thing of the functionally two-dimensional graphene oh, that bad boy would be incredible however graphene is very flexible so this might defeat the purpose. However something tells me that if the graphene were to be bio mineralized to be interwoven with the nacre be beneficial.

[@leightonspillane8275]

as a sophomore in high school, i honestly dont know why or how i understand any of this but i want to major in biochemistry and this is something that i really want to work on. since the first time i saw spider man ive always wondered if this was possible. thank you for proving to me that it is and inspiring me to work on something similar myself

[@HanshiroParker]

Peter watching this specific video, with Tony: “wRiTe ThAt DoWn!!!”

[@altofmoleperson]

Why am I watching this, my experience with biology is “the mitochondria is the powerhouse of the cell” and “sometimes, plants eat the sun.”

[@imcoolerthanallofyou7751]

I’m imagining a plant just biting a giant chunk out of the sun

[@altofmoleperson]

@@joshuanetanel9174 Of course, the kale kills.

[@Grumpy_old_Boot]

Soylent green is people too.

[@patricknkwo652]

lol, same, I'm just curious to see what emerges in the next 25 years from him & this field

[@hahayoucantstopmehawokenha4059]

i would love to see a plant eating the sun

[@PauloOlveira]

Just leaving this out here: A silk/graphene composite generating yeast that survives high salinity would be prime candidate for regenerating tidal turbines

[@ninjanyan1579]

I understood all of those words separately and am proud of myself for that.

[@ThisLockIsJammed]

If this is true, on top of making it a commercial clothing or building material, this guy could be looking at a Nobel.

[@wedmunds]

I think that could do a lot more than just repairing turbines

[@thecallankids4718]

While it could do that, would it not also coat the ocean floor in carbon doped silk?

[@Gign--a]

@@thecallankids4718that I’d assume degrades faster than thousands of years

[@jfischer507]

Somewhat new to the channel, I've been watching a lot of your videos lately. First time I've seen your face. Always interesting when someone reveals themselves like this. You look good, my man! Just waiting on AvE to do the same. In all seriousness though, keep up the good work. I love the commitment to clear and precise narration, kinda like NileRed and Applied Science. It's what makes your content great.

[@jmikronis7376]

Microsilk Bolt Threads has done it. They can make a bulletproof vest out of spider silk in their process. It’s a lightweight fabric.

[@bumpket]

The dislikes are just the other spiders being jealous about this amazing silk.

[@greengreen110]

And the big corps geting mad because the plasmid is open source

[@aflyingpumpkin1073]

This was the fastest I have ever clicked on a notification.

[@pocholomanalac9561]

Same

[@nikolaytekuchev136]

First- Oh fuck

[@foolwise4703]

Dito

[@Orion791]

Right lol

[@samdwichenjoyer]

God dude this is so cool. Literally coding shit into reality. Absolutely mindblowing work you're doing here, despite how arguably silly the end goal is

[@Idk-wm1bc]

I like your videos so much because you take stuff that was boring in biology class and make it super interesting

[@thethoughtemporium]

That's how I passed my bio class in highschool. My teacher was painfully boring and I knew I'd fail the class if I couldn't find the interesting bits. Then realized most teachers are just bad, and it's actually an amazing subject

[@nostradamus9132]

​@@thethoughtemporium what happened to this project? Are you still continuing it. Looks amazing.

[@Froyorionpop]

​@@nostradamus9132 I wonder this as well

[@ramabary]

Make the web cure faster is hard enough. Let alone: 1. It can carry the man size weight 2. Produce a length of string 3. Infinite strings.

[@sonofanutcracker4207]

4. Become spiderman

[@darknobz3560]

@@sonofanutcracker4207 5. Great responsibility

[@urtooslow4415]

@@darknobz3560 great stress and late rent debt

[@benbruder]

@@urtooslow4415 7. *MENACE!!!!*

[@praddhyumnmani7925]

@@benbruder 8. Get forgotten by everyone

[@nezgroul666]

As a molecular biologist, the explanations in this video are better than most of my uni courses. Difficult principles made simple. Explaining something so that someone uninitiated can understand is a gift. Kudos for the job well done, your countless hours spent on this project were not in vain, sir!

[@lourock_]

The tensile strength of that is off the charts

[@aloysiuskurnia7643]

Vortexing nad pipetting up and down is one _hell_ of a difference of force.

[@kingarthurthe5th]

Giving a whole new meaning to “silky smooth beer”

[@acompletelynormalhuman6392]

Congratulations I know you've been working on this project for a while and it's really exciting to see you succeed

[@ambientphotoph]

this is mind-blowing.

[@slimeinabox]

It has been said that when you understand the subject well, you can explain it simply. I felt like I understood everything you said. Well done! (btw i wasnt even watching, i was playing ultrakill while listening to this)

[@Zellan_Kishin]

me too bro