Immunohistochemistry (IHC) workflow [WEBINAR]

  Рет қаралды 624

Rockland Immunochemicals, Inc.

Rockland Immunochemicals, Inc.

Күн бұрын

Learn the steps of IHC workflow with Rockland Product Manager, Renee Salvo, Ph.D. This overview will take you from tissue preparation to visualization with clear and concise explanations of both enzymatic and immunofluorescent assays.
Contents:
00:00 - Intro / What is IHC
00:32 - Advantages and Disadvantages
01:35 - IHC workflow for tissues
02:07 - PE Tissue Preparation
05:48 - Frozen Tissue Preparation
07:30 - Antigen Retrieval
09:36 - Quench and Blocking
12:28 - Review
12:57 - Enzymatic Antibody Incubation & Washes
16:46 - Stain & Counter Stain
19:26 - Recap of Advantages and Disadvantages

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