Introduction to Metabolomics

This is the first module in the 2016 Informatics and Statistics for Metabolomics workshop hosted by the Canadian Bioinformatics Workshops. This lecture is by David Wishart from University of Alberta (tmicwishartnode.ca/).
How it Begins by Kevin MacLeod is licensed under a Creative Commons Attribution license (creativecommons.org/licenses/...)
Source: incompetech.com/music/royalty-...
Artist: incompetech.com/
Table of Contents:
00:10 -
00:34 - Learning Objectives
01:57 - Schedule
02:15 - The Pyramid of Life
08:07 - What is Metabolomics?
08:47 - What is a Metabolite?
10:58 - What is a Metabolome?
12:27 - Different Metabolomes
14:48 - Human Metabolomes (2015)
17:27 - Theoretical Human Metabolomes
19:06 - Why is Metabolomics Important?
19:17 - Small Molecules Count…
23:53 - Metabolites Are the Canaries of the Genome
24:20 - Metabolomics is More Time Sensitive Than Other “Omics”
25:51 - Metabolism is “Understood”
26:16 - The Metabolome is Connected to all other “Omes”
27:04 - The Metabolome is Connected to All Other “Omes”
28:04 - Metabolomics Enables Systems Biology
28:37 - Metabolomics Applications
29:08 - Metabolomics Methods
29:45 - Metabolomics Workflow
35:03 - Comparing “Omics” Coverage
36:11 - Why Metabolomics is Difficult
37:08 - Metabolomics Technologies
38:10 - Chromatography
38:15 - Chromatography
39:18 - High Pressure (Performance) Liquid Chromatography - HPLC
39:58 - HPLC Modalities
40:56 - HPLC Columns
41:30 - Reverse Phase Column
42:32 - HPLC Separation Efficiency
43:08 - HPLC Schematic
43:49 - Gradient HPLC Schematic
44:27 - HPLC of a Biological Mixture
45:16 - Gas Chromatography
45:42 - Gas Chromatography
46:27 - TMS Derivatization
48:12 - Gas Chromatography
48:43 - GC-Columns
49:12 - Retention Time/Index
51:15 - Compound Identification and Quantification
52:03 - GC-MS Chromatogram of a Biological Mixture
52:23 - Mass Spectrometry
52:44 - Typical Mass Spectrometer
52:51 - MS Principles
53:18 - Mass Spectrometry
53:42 - Different Types of MS
54:12 - Masses in MS
55:14 - Isotopic Distributions
56:13 - Isotopic Distributions
56:52 - Mass Spec Principles
57:15 - Typical Mass Spectrum
57:42 - Typical Mass Spectrum
58:08 - Typical Mass Spectrum
58:35 - Typical Mass Spectrum
58:43 - Resolution & Resolving Power
59:00 - Resolution in MS
59:44 - Resolution in MS
01:00:21 - Resolution/Resolving Power
01:01:08 - Mass Spectrometer Schematic
01:01:53 - Different Ionization Methods
01:03:56 - Electron Impact Ionization Source
01:04:43 - Electron Impact Ionization
01:05:14 - EI Fragmentation of CH3OH
01:05:43 - Electron Impact MS of CH3OH
01:06:11 - Soft Ionization Methods
01:06:39 - Electrospray (Detail)
01:07:18 - Electrospray (Detail)
01:08:14 - Electrospray Ionization
01:08:38 - Electrospray Ionization
01:08:55 - Electrospray Ionization
01:09:27 - Mass Spectrometer Schematic
01:10:27 - Different Types of Mass Analyzers
01:11:54 - MS Mass Accuracy
01:12:47 - Mass Chromatograms
01:13:43 - Mass Chromatograms of Biological Mixtures
01:14:13 - NMR Spectroscopy
01:15:10 - Explaining NMR
01:15:55 - Principles of NMR
01:17:04 - Protons (and other nucleons) Have Spin
01:17:46 - Each Spinning Proton is Like a “Mini-Magnet”
01:17:58 - Principles of NMR
01:19:23 - Bigger Magnets are Better
01:19:59 - A Modern NMR Instrument
01:20:37 - NMR Magnet
01:21:28 - NMR Magnet Cross-Section
01:22:23 - An NMR Probe
01:22:59 - NMR Sample & Probe Coil
01:23:12 - 1H NMR Spectra Exhibit...
01:24:30 - Chemical Shifts
01:24:51 - Characteristic Chemical Shifts
01:25:10 - Assigning Simple NMR Spectra
01:25:58 - Assigning Simple NMR Spectra
01:26:15 - NMR Spectra Need “Fixin’”
01:26:53 - NMR Spectra Need “Fixin’”
01:27:18 - NMR Spectrum of a Biological Mixture
01:27:47 - Technology & Sensitivity
01:30:00 - Comparison
01:30:21 - Comparison
01:31:04 - What’s Possible
01:31:45 - 2 Routes to Metabolomics
01:32:11 - Profiling (Untargeted)
01:32:59 - Quantitative (Targeted)
01:35:59 - From Spectra to Lists
01:36:15 - From Lists to Pathways
01:36:31 - From Pathways & Lists to Models & Biomarkers
01:36:45 - Key Informatics Challenges in Metabolomics
01:36:59 -

[@yangpu6984]

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[@akrrrs1739]

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[@josmontero4627]

Thank you very much for the contribution!

[@bolajifoyeyemi375]

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[@mebratubitew3904]

I really appreciate your help for letting us know about metabolomics

[@susankim9305]

Thank you for posting :)

Amazing this lecture!!