Melanocytic Dermpath Basics: Immunohistochemistry

A complete organized library of all my videos, digital slides, pics, & sample pathology reports is available here: kikoxp.com/posts/5084 (dermpath) & kikoxp.com/posts/5083 (bone/soft tissue sarcoma pathology).
Part of my (in progress) video series about the pathology of melanocytic skin lesions. This video discusses my approach to using immunostains (IHC) on melanocytic lesions with multiple examples to compare and contrast.
Topics Discussed: (time stamps will be added here eventually)
(***if you want to post time stamps in the comments to help me out, I'll copy-paste it here and give you name credit!***)
Which IHC stains I like for different scenarios:
Lentigo:
Lentiginous nevus:
Dysplastic (Clark's) nevus:
Melanoma (lentigo maligna type):
Melanoma in situ vs melanocytic hyperplasia in sun damaged skin:
Melanoma in the dermis (metastatic pattern):
Other videos that will help you better understand this topic:
Normal Skin Histology video: kikoxp.com/posts/3660
Nevus 101 video kikoxp.com/posts/3740
Melanoma 101 video kikoxp.com/posts/3764
Additional suggested reading:
Check out my #dermpath Survival Guide book: bit.ly/2Te2haB ‬
- Nathan T. Harvey, FRCPA; Benjamin A. Wood, FRCPA. A Practical Approach to the Diagnosis of Melanocytic Lesions. Epub ahead of print. Free PDF here: www.archivesofpathology.org/doi/pdf/10.5858/arpa.2017-0547-RA?code=coap-site
Disclaimers: As I said in the video, distinguishing nevus from melanoma is serious business. My video does not replace the need for getting an expert consultation should you encounter a difficult melanocytic lesion in your practice. Also, melanocytic dermpath has areas of controversy with differing strongly held points of view. by various experts in the field. This video represents MY current views as of August 30, 2018. My views have changed since I started practice, and I suspect (and hope) that they will continue to evolve over time. You (or your mentors) may do things differently than I do. That's ok by me. I'm just sharing the way I currently think of melanocytic lesions with the hope that viewers may find it useful. My goal is to educate, not to create dogma.
This video is geared towards medical students, pathology or dermatology residents, or practicing pathologists or dermatologists. Of course, this video is for educational purposes only and is not formal medical advice or consultation.
Presented by Jerad M. Gardner, MD. Please subscribe to my channel to be notified of new pathology teaching videos.
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Facebook: / jmgardnermd

[@JMGardnerMD]

A complete organized library of all my videos, digital slides, pics, & sample pathology reports is available here: kikoxp.com/posts/5084 (dermpath) & kikoxp.com/posts/5083 (bone/soft tissue sarcoma pathology).

[@tamilarasirenganathan8243]

Thank you so much. I have learned so much from watching all these lectures. Great help. Really appreciate. Now I am more confident when I am looking at melanocytic lesion.

[@zhengxiaomin3638]

Great! It is exactly what I am looking for. Thank you, Dr. Gardner

[@janiranavarro]

Thanks Dr Gardner for this video!! Super helpful and educational!!

[@sevtaptolaman4812]

Thanks very much!These videos make me change my perspective

[@gabrielaacuna4036]

Great!!! Can't wait to watch it! Love your videos! Thank you so much :)

[@johnfazio2892]

Great review of melanoma IHC. Very helpful.

[@evelyne7071]

You’re a really great teacher !

[@Nicollas16]

Which IHC stains I like for different scenarios: 2:06 S100: 9:30 Melan-A (MART-1): 11:45 HMB-45: 13:17 SOX-10: 16:11 Lentigo simplex: 18:20 Lentiginous nevus: 27:17 Dysplastic (Clark's) nevus: 30:39 Melanoma (lentigo maligna type): 50:14 Melanoma in situ vs melanocytic hyperplasia in sun damaged skin: 58:11 Melanoma in the dermis (metastatic pattern): 1:11:04

[@JMGardnerMD]

Thank you!!!!

[@paulo4856]

Thanks, Jerad.

[@luisfernandocardenascardon8100]

Great video!!!

[@harimartinez1039]

Thank you ☺

[@jennah19]

Thank you!!!

[@AhmedGamalPost]

Thank you

[@MauriceMQLD]

I honestly can't thank you enough for what I've learned from your videos. You have demystified many concepts in dermpath for me. Greatly appreciated.

[@JMGardnerMD]

Thank you! Such a nice compliment.

[@arkarhtoo7797]

Thank you. It’s a very nice lecture. I learn a lot from your videos. I have a question. In case of margin assessment for melanoma in situ, can PRAME be a better way of assessing than other markers?

[@JMGardnerMD]

I haven’t tried that but I think it would be difficult to use in that way. I see PRAME stain background melanocytes sometimes especially in sun damaged skin so that would Make it hard to use for margins I think.

[@goharrehman7710]

the green stain is from grssing

[@pavelboico9283]

Today our pathologists recommended PRAME to differentiate melanoma. Is it useful marker?

[@JMGardnerMD]

Yes I like it a lot. It’s not perfect but can be very helpful in select cases. I had no experience with it at the time I made this video which is why I didn’t include it.

[@franciscosimental4930]

Dr jerad: why does "melanoma in situ" not appear in the histological classification of melanoma? It seems to me that it is a term that is not widely accepted because it does not meet defined criteria, does it?

[@JMGardnerMD]

It’s not a histologic subtype. It’s more like a stage. It just means melanoma that is confined to the epidermis and hasn’t invaded.

[@franciscosimental4930]

@@JMGardnerMD thank you

[@JMGardnerMD]

Also check out my videos on melanocytic nevus basics (kzbin.info/www/bejne/oHy9daSAmt9soNk ) and melanoma basics ( kzbin.info/www/bejne/bn-Teo2QpatrqtU ). And be sure to read the disclaimer in the video description above.

[@Woffle8340]

7 Foot rats, rats along his rats. when he calls your rats, it all fades to rats!

[@pathologygeek7360]

You can get a great Dermatopathology review course of 15 lectures from the Oakstone institute or payloads store: m.kzbin.info/www/bejne/gJ6xnJiohJh3ibM store.payloadz.com/details/2573416-movies-and-videos-educational-dermatopathology-oakstone.html