Key Differences in the Use of Methanol and Acetonitrile in Liquid Chromatography

Methanol and Acetonitrile are organic acids commonly used as the mobile phase in reverse-phase chromatography. The properties of these two organic acids differ, and this video outlines key differences to keep in mind when using methanol or Acetonitrile for analysis.

[@thilpanneerpanneer2327]

Crystal clear teaching.No one teach like this sir.Salute ur teaching style.Pl put video for HPLC trouble shooting

[@bestdancevideos2253]

Superb Superb Superb Video Sir 💓💓💓

[@seyedmohammadseyedkhademi2454]

Very very professional and understandable Please go on about troubleshooting of HPLC Thanks for video

[@bestdancevideos2253]

How To Seperate Structural and Positional Isomers Sir Please Upload Videos Sir

[@kirana.g4654]

is it necessary to use same column for same drug product analysis?

[@seb00zi]

For the faster elution of Benzoic acid with ACN, because of the extra interaction with the solvent shouldn't it spend more time at the mobile phase and come out a bit later instead? I didn't really understand why that pi-pi interaction makes the benzoic acid come out faster. Thank you so much for the video btw!

[@jaishreemali9443]

Because ACN is a mobile phase and not stationary phase

[@kirana.g4654]

methanol and acetonitrile are organic solvents.

[@pharmagrowthhub3083]

Yes, both of them are organic solvents with acidic property, one is APROTIC (ACN) and other PROTIC (MEOH)

[@kirana.g4654]

@@pharmagrowthhub3083 sir, please check the description of the video. It says methanol and ACN are acids

[@sthakur8780]

Sir, is it possible to contact you......? Plz sir....Provide mail Or something else so that ..We can share our doubts ....