Kurt Thorn (UCSF): Confocal Microscopy

GET UPDATED VERSION OF THIS TALK AT: www.ibiology.org/talks/confoc...
Confocal microscopy is a powerful technique for acquiring three-dimensional images of biological samples. Here I discuss the basic principles of confocal microscopy, with specific discussions of the operation of laser scanning and spinning disk confocal microscopes and of their application to biology.

[@nirmaljayadev]

That's just extra ordinary talent. To make such a complicated thing so simple

[@mrmatz408]

Extremely thorough and well-prepared discussion of a topic in which I had no previous understanding. Totally great!

[@andrewareva4605]

If only this video was in higher resolution...

[@scicommlab]

+Andrew Areva We re-recorded this video a couple of years ago with higher resolution. Check it (and a lot of other microscopy videos) here: kzbin.info/www/bejne/Z4KYh2dqZ7tqrqs

[@brandond8488]

@@scicommlab Great use of super-resolution imaging 👀

[@ErichAmMeer]

try confocal

[@sylviahadai5291]

Thank you so much for this excellent presentation!!! ❤

[@mrunalsakharkar5463]

Excellent video. Very easy to follow. Thank you!

[@rob66181]

Really nice video, thanks for uploading this. I suggest this to anyone who is going to use a confocal as its going to make your life a lot easier and will make it all a lot clearer. Thanks

[@xadroit]

Thanks for an excellent, easy to follow introduction to confocal microscopy! That would have taken me hours to read:) Keep up the great work.

[@priye19]

Thank you. Very precise and informative.

[@hectorjosecardona]

Excellent presentation, very didactic, thanks Kurt

[@sylviahadai5291]

Thank you so much!!

[@MohammadAlKobaisi]

Very helpful, thanks!

[@Nanomachines5on]

Good explanation of the diagrams. Thank you!

[@amarmirza08]

Thank you for making this video.

[@nishanthsurianarayanan296]

Thank you for the amazing explanation

[@maxencehannard1988]

Thanks for this wonderful video

[@KeyManiacLad]

Excellent presentation!

[@mindlessgreen]

Fantastic. Very well explained.

[@scientia_potentia_est]

awesome vid, thanks to iBiology and Nikon! Wish the resolution was higher though. Crazy to think that after a few years (from the date of this video in 2010) laser scanning confocal has gotten so efficient with the new generation of insanely fast galvano-resonance hybrid scanners that spinning disk is getting phased out since you can get pretty much very close frame rates at comparable resolutions (something like 30 fps, 512x512) without the small compromise in optical sectioning that you get in spinning disk (which is not as bad as some people make it out to be though). Although 2-photon's still the boss, if you've got the $$$$$$ hehe

[@caminhodeperfeicao273]

Thank you is very much for the video!

[@djinnahwell]

Thanks, Thanks ! So great video.

[@garrystewart3836]

Great help. Thanks!

[@painlessvoodoochild]

Thanks for great explanation

[@Maryam85ful]

Very nice video! thanks for uploading :)

[@dolet8]

explained very clear.

[@paolofagherazzi1684]

Thank You very much

[@aman0311tisf]

Excellent work Kurt!!! I've addad a link on my blog -

[@peterhartman55]

Great video.

[@VSerman13GR]

Thank you!

[@9haylee]

Excellent!

[@leixiao169]

fantastic!!

[@charme2377]

excellent video..i have a question please,i want to do a fluorescent exerience with confical and 1 EMCCD camera ..what is the difference between using 2 camera or 2 ...thank you

[@sorrzietgiest]

very clear... helped me a lot... thanks..:)

[@MN-mj9ok]

Thank you for the very helpful video. I have one question.1. Why do we limit to 100µm imaging depth by confocal microscopy? Does imaging depth get better by decreasing pinhole size?

[@ahmadnabawi5060]

a life saver !

[@antoniodimaggio4779]

this is such a good one! Thank you so much

[@djalitanaful]

I am stuck, can't get it. trying hard to understand how to see molecular interactions using fluorescence and confocal microscope. is there any possibility to do a video before this and explain basics of how light interacts with specimen?

[@gregrobertson9732]

Nice one!

[@vman049]

Awesome!

[@zhangcanding3263]

Great! Thanks

[@xplorer9]

awesome!

[@uvesca]

yes... Awesome!

[@sasamaster]

Excelente! Muchas gracias, esto me ayudó mucho. :-)

[@xtremetom180]

very interesting thanks

[@BushCampingTools]

Confocal and multi-photon way to go! Spent years in research using, and later selling LOL!

[@tactileslut]

This sounds inspired by early innovations in the history of television.

[@psamrai7]

is there a difference between the dichromatic mirror and dichrioic mirror?

[@megatog2011]

Very nice presentation there. However, if it was in HD (720p or better), I would have seen and maybe understood more ;)

[@FatemahRezayee]

Thanks a lot

[@anonviewerciv]

13:45 Apparatus. 18:28 Spinning disk. 22:30 Multi-photon.

[@doktonic1370]

hell yes

[@sorrzietgiest]

wait.. working of spiral spinning disc is not very clear..

[@FryChicken]

Confocal Microscopy starts at 3:50

[@edwindeleon1130]

need elctron microscope for hiv diagnoses

[@sorrzietgiest]

spinning spiral mirror working is not very clear

[@q0L0b]

LIKE.

[@humbertosmith6985]

Opino. Que. Diyegnnoñojia. Es. Maravillosa. Pero. Soy. Una. Simple. S🎃😊😅😂😂

[@kevinggyy]

like

[@mmartin5816]

If only microscopists and histology people added scale bars. Don't show me an image without context!

[@lotharmayring6063]

unsharp unsharp unsharp unsharp unsharp ..................................

[@venividiviolini6459]

"you can see that there is stuff that is clear and sharp and in focus.." yeah.. nah not really :'D

[@preynoldssco]

Why is this potato quality?

[@darlantro]

uploaded 5+ years ago. KZbin had harsher compression requirements back then.

[@darlantro]

also, probably an old camera

[@sylviahadai5291]

Thank you so much for this excellent presentation!!! ❤

[@sylviahadai5291]

Thank you so much!!

[@lizs7827]

Very helpful, thanks!

[@hitgucvkj]

Awesome!

[@redpinkgreenandblue]

very helpful, thank you :)

[@xplorer9]

Awesome!