Microscopy: Two Photon Microscopy (Kurt Thorn)

[@hughxiao7078]

10:10 was the moment the advantage of two photon microscopy clicked for me. 16:37 is another great point.

[@OsamaOSadolla]

mvp

[@1239752]

Much better than my university's newbie lecturer(surprisingly hired at US top 30 university) Wish he watch this and learn how to teach.

[@alvaroperona6134]

Thank you so much for this video!! I was 100% lost on how the multiphoton system worked.Very clear explanation.

[@abdullahiibrahimuba1137]

Very complicated but well explained technique. Good job!

[@TheWupperdo]

Excellent explanations--didn't realize how much I wanted to use multi-photon microscopy until seeing this video!

[@fernandamonteiro5181]

EXCELLET explanation!! no doubts left behind, very well organized and fulfilled with information! great job!

[@sr2109]

Using the talk for preparing for exam. Really helpful! Thank you

[@sashatolstoy531]

Excellent and easy-to-follow!

[@ClaraL27]

SO clear and helpful- thank you!

[@johnbates2709]

Great presentation, very clear, thank you

[@beflatminor]

Very well explained and helpful. Thank you!

[@WALLEHua]

excellent lecture! very clear and easy-to-follow

[@colinbarnes705]

Still a great vid 10 years later

[@TheHerofruitlunch]

Thanks for this, the basic principles are clear for me now!

[@kadamnagesh5155]

Very good information.. Easy to understand.. Thanks

[@muthuveljothi4619]

Extraordinarily explained 👍🏾

[@Kimya_el_tabkh]

Great lecture. Thanks

[@1point689]

Great lecture!

[@deebadubbie]

Thanks for a great talk!

[@gVaiwa]

Easily comprehensible, thanks a lot :)

[@samanthaamat4976]

Very thorough, thank you!

[@danielarmbruster94]

Awesome, thank you!

[@gillenzfluff8380]

Amazing stuff thank you.

[@charme2377]

excellent video..i have a question please,i want to do a fluorescent exerience with confical and 1 EMCCD camera ..what is the difference between using 2 camera or 2 ...thank you

[@frankclausen2506]

wow - It's so nice to se another way for using second harmonic, that just PTK. dubling.

[@mariaguadalupedelrocioherr9722]

Thanks :D ... Good explanation.

[@milophilic5927]

Super helpful and logical! thanks !

[@crewberggren7669]

10/10 In every category

[@prananditabiswas4599]

Thanks for the excellent explanation. I have a doubt : two photons are two different quanta, even if they arrive simultaneously, would they not excite two different dye molecules with the same energy and not the same molecule with twice the energy ?

[@kunlin579]

From my understanding, that's the trick of 2 photon, at the focal plane, the density of the photons is so high that 2 photons will hit the same molecule. Outside of the focal plane, the likelihood of 2 photons hitting the same molecule goes down, therefore photo-bleaching is minimized outside of focal plane

[@poodook]

There is no real upper level (excited-state) corresponding to a single photon’s energy and therefore there will be no absorption.

[@artsmith1347]

Agreed: 1) Excellent explanation. 2) I had(?) the same doubt. It doesn't square with my understanding of how excited states happen ... the reaction of a molecule to being hit with half the energy needed to excite it would be: "hold my beer." It appears my understanding is incomplete because they are making it work.

[@oludhe7]

Very good presentation

[@aidated]

Brilliant! Thank you!

[@artsmith1347]

So this is ionizing radiation at wavelengths far longer than those for x-rays?

[@HayagreevSivakumar]

thank you

[@poodook]

22:40-22:45 this is not truly the reason, in my opinion. A more appropriate explanation would be that the two-photon cross-section is extremely low and thus high intensities are a necessity for inducing two-photon absorption. Two photons will always be present, but the absorption will not be stimulated unless there is a high density of photons, owing to the low cross-section. In fact, this cross-section will become orders of magnitude weaker for even higher multi-photon events.

[@hellenishikawa7841]

HI it will be possible to have the ppt presentation for didactic propose? It will be nice! Thanks Dr. Hellen Ishikawa-Ankerhold LMU Munchen

[@iBiologyTechniques]

Hellen Ishikawa Dear Dr. Ishikawa-Ankerhold, unfortunately we are not able to give out the powerpoint presentations. If there are particular slides you are interested in let us know and we'll see what we can do!

[@Rhioah]

Thank you!

[@Abyssale90]

THANK YOU!!!

[@dr.vannostrand411]

any idea where i can get that graph at 6:25? the link to it doesn't work

[@iBiologyTechniques]

Hi Five-Sigma! You are right - the link doesn't seem to work anymore. I contacted the speaker and he suggested these two similar (but not the same) graphs: www.nature.com/icb/journal/v88/n4/fig_tab/icb2009116f1.html research.stowers-institute.org/microscopy/external/Technology/NLO/index.htm Sorry that we cannot find the exact one used in the talk!

[@dr.vannostrand411]

Thanks very much! Those graphs are great!

[@edoardodimprima7519]

@@iBiologyTechniques the links seem broken now. Thanks a lot for the great lecture!

[@zainhassep7908]

Excellent

[@cokecamilo]

Are you from the future!?

[@ashutoshmishra1554]

Who dislikes such videos

[@disisnups]

good explanation, he speaks like sheldon cooper :)

[@skoolscribe3918]

Next time just present the source to your data and include user interactivity

[@skoolscribe3918]

So the goal is to measure photon from extraction rather than defining photon by compression however a photon compression engine will suffice most excellently for any security system weather it be for a spacecraft or for a human body.

[@souvikces]

Wonderful presentation, except the occasional tongue-clucking "sucking" sound (for instance, see 25:06, 26:19, 26:38, 26:50). This sound can be annoying for many audience. Thank you and I hope you take this suggestion positively.

[@rockgaming1440]

This is violating Einstein's statement for photoelectric efect

[@Manish00333]

why is he so annoyingly excited by this

[@BrandonSLockey]

lmaoooo

[@artsmith1347]

Why are you annoyed? It is a well-delivered and interesting presentation.