Learning about the Transmission Electron Microscope

[@SardiPax]

Really fascinating. I'd love to see more such in-depth videos on equipment most of us will never use. On science TV programs we barely get a glimpse of equipment like this let alone hear the details of how it is used. Keep it up :)

[@oisiaa]

Absolutely! I would love to have an entire youtube channel about nothing other than industrial equipment operation.

[@dan110024]

oisiaa Totally! Filmed and produced by only Destin, though ;)

[@miguelsalvado8725]

Timeworthy, must say. Very clear explanations from Dr. Simmons and right on-time questions to maintain a dialogue instead of just a simple boring presentation. Congratulations and keep delivering more like this!

[@MrRobinhalligan]

Many thanks to Dr Simmons that was fascinating, looking forward to more

[@OrdnanceTV]

Absolutely love these more in-depth videos with professionals, allowing them to describe the intricacies of their work.

[@flydiscovery]

That was FASCINATING. I'm a (biomedical) engineer who has ended up doing microscopy full time so this is all right up my alley. I deal in much larger real estate, cells & tissues, and have never had the opportunity to get into electron microscopy.

[@LzBy1]

Thank you Dr. Simmons for taking the time to share this. This was awesome. Destin keep being awesome.

[@bsinger182]

Curiosity and amazement are fun and infectious. Destin, both of your channels are among my favorites on youtube.

[@gregmossed]

You've got a real knack for asking a question shortly after I think it, I really appreciate that. Big knowledge bombs like your videos are super satisfying.

[@jschwebs]

Love the microscope videos! We use microscopy to look at paint sample layers in historic buildings for preservation. Fun stuff!

[@StephandaSilva]

I like where he says "It's big. They're about 300nm."

[@DaffyDaffyDaffy33322]

pretty sure he said "pox virus"

[@bourkept]

I always see the video title and think that it will be too complicated or boring but then I click and watch the video and it blows my mind.

[@TechLaboratories]

I love that both SEMs and TEMs are built around the principles of the Cathode Ray Tube - Similar electrical processes for focusing and firing electrons into a vacuum are used, but their purposes and end results are vastly different!

[@Niosus]

That's the cool thing about science. If something works, it will keep working! No need to reinvent the wheel :)

[@SgtClueLs]

Awesome video. Thanks Dr. Simmons.

[@josh9493]

just want you to know this video is helping me revise for a big exam and it was so helpful!!

[@cruiseknallkul6073]

You can make anything exiting and interesting. Incredible channels, both smarter and funner! Great job man

[@poppysomniferum5844]

Thank you so much Dr. Simmons for explaining everything so thoroughly and also thanks funnereveryday for posting and interviewing. Very educational.

[@stan.rarick8556]

Excellent post. More detail than you usually do

[@zerokmatrix]

Amazing, I try to and love to learn new information every day and this is by far the coolest amount of information I've learnt in a while. Great questioning too.

[@soptamer]

11:11 the guy's face after that "whatever" lol

[@PoopShitz]

11:17 should be a meme haha

[@PodbevsekMiha]

This video was amazing. Everything was explained very nicely and if it wasen't, you asked the right questions. Thank you!

[@Manodragon]

Love it, love it, love it. As a medical student in my first year, seeing how the electron microscope works is amazing! Please post more footage if you have more and thanks for this one. :)

[@charlescabana7849]

Brings back a lot of memories.

[@deepsuthar9699]

Thank you for sharing this Destin, it was incredible to learn and watch :)

[@maddybelo]

Great video Destin, thanks for sharing it with us! Love getting a 'behind the scenes' type look at this sort of stuff. In my undergrad biomed degree we did quite a bit of histology with light microscopes, so it's cool to see how he uses a TEM for other types of samples.

[@danheidel]

Ah, I used to use a TEM all the time back in grad school. A pain to use but nothing beats it for getting a ton of data from your sample.

[@Anirban456Mandal]

Thanks for such cool and amazing stuff Destin

[@franciscolagunas6633]

Destin, we have an abberation corrected scanning transmission electron microscope at the lab I work at. We can get atomic resolved images of materials, yeah, we can "see" atoms! Also, we are fitted with a high angle annular dark field detector which acquires images with atomic weight contrast. We get some really fascinating images. If you're interested in coming by I can talk to the Lab Manager to see if we can give you a tour.

[@Rickmakes]

Why wasn't this video on SmarterEveryDay?

[@destinsandlin7122]

Because it's a simple question/answer format. It's for the people who really want to learn and experiences stuff with me... like you guys here at FunnerEveryDay

[@RMoribayashi]

Hey Destin. Great video. When you said "coordinate measuring machine" it brought back a memory of something I saw on NASA TV. Maybe you can give me a lead on where to find out more about it. 25 years ago NASA TV had a lot of unused time on it's satellite transponder. They often used it for "in house" programming to all the centers around the country. One was a series of guest lectures on metrology. The speaker was one of the best teachers I'd ever seen. I've been fascinated with the science of measurement ever since. I'd love to know who he was.

[@dondacurator]

tne out of ten even though the electron guns were not explained and the lenses (magnetic fields), I love how it was just layed back and chilled yet so informative video mazeltop BRILLIANT I say

[@russtex]

Awesome video! Very interesting!

[@EdanHabel]

This is a great video, I'm getting to use one of these soon and this has been the best video I've found so far letting me now what I'm in for :)

[@qoaa]

This was a triumph. I'm making a note here: HUGE SUCCESS. It's hard to overstate my satisfaction. Aperture Science We do what we must because we can. For the good of all of us. Except the ones who are dead. But there's no sense crying over every mistake. You just keep on trying till you run out of cake. And the Science gets done. And you make a neat gun. For the people who are still alive.

[@jeremyj.5687]

A lab like this has been my home for half a year in the past. I really got that "hardcore-science" feel Destin mentioned, it was an awesome time. Kind of a shame I moved on from that.

[@firemarshal1bill]

I subscribed to your second channel earlier today, now I'm glad I did.

[@TylerTAF]

As a biology student I find it really cool to see the gray between biology and physics. The term indexing came up a few times, once when talking about the ruby in the SEM. Is this refractive index? I'm familiar with the concept with light, how does that work with electrons?

[@danheidel]

Indexing just means making sure the sample is locked into the same position every time you put it in the scope. There's a precisely machined receptacle in the sample manipulator that fits the ruby and therefore each time the sample holder is inserted, it repeatably goes back to exactly where it was before.

[@TylerTAF]

Interesting, thanks! So I'm assuming that the near perfect and predictable crystal structure is the basis for this kind of orientation?

[@danheidel]

Nah, just being really hard. If it were metal, the clamped point woul wear over time and the connection would lose accuracy.

[@AwayParkour]

VERY informative! Thank you very much for sharing this!

[@Leopr1]

I have experience with SEMs but no so much with TEMs so it was very interesting video.

[@mkirefu]

thanks Destin great video

[@stephanieward8822]

Destin you should learn some more about histology. I work in a histology lab and it's very interesting. Most people have no idea what happens to their organs when they are removed.

[@nishanks93]

This is so familiar. I've seen one at my university (ANU, you've been down here!). You should also do confocal microscopes. They pretty amazing as well, especially for generating 3D images.

[@dbauernf]

That was amazing. :-x I had no idea such things even existed!

[@Masaladas]

Hey Destin, this is kindof unrelated to the video but theres this really cool thing you can do in TD (Total Darkness). It involves Life Savers Wintergreen flavor. There is a light produced when you chew on them in TD. Ive done it on some caving trips and stuff and it's really cool. You should try it out and see for yourself!

[@TeemarkConvair]

VERY interesting, thanks

[@priyanshu7821]

Awesome vedio sir

[@MrJoshimitsu1]

"look at me and my ... science"

[@Anirban456Mandal]

1:47 -- so cool

[@ArcticCustomProps]

This is very interesting stuff. Way over my head, but interesting.

[@israelhydroponics8659]

great video. thanks

[@JamesH_Gp]

Hey Destin(I think that's how to spell it :p)! I was the team lead for Genesis of Robotics! Just Wanted to say awesome sauce on this vid and i hope to see more from you soon!

[@Absolution55]

Awesome video Destin=D

[@MusicByNumbersUK]

Love it! very interesting :) thanks

[@zipper18b]

I am a disabled veteran with a severe tbi so learning is hard for me now but you make it easier the way you go about it is there any chance you can do a series on hho or hydrogen generators I was trying to build a 36 or 48 volt set up that would run around 15,000 hz off a pwm let me know what you think I believe we can run cars off water Thanks again

[@guri9255]

i've got a question, how does chemists(i think), design drugs to fight specific deseases? i mean, they see the germ's structure, design a drug to react with it... how do they do it? how can they design something so small, yet make so many copies of it?

[@fuzzynipple47]

WOW... I feel so dumb, my job is so boring after watching this.. That is so cool!

[@ericvilas]

8:46 ok, that's an aperture, and he's showing us what it does... I guess you could say he's doing... ...Aperture Science. ....(probably singlehandedly the worst pun I ever made)

[@ArcticCustomProps]

This was a triumph. I'm making a note here: Huge success.

[@ericvilas]

It's hard to overstate my satisfaction.

[@jeremyj.5687]

You monster.

[@nate2d2]

I just realized that this channel is called Funner Every Day and not Funnier Every Day.

[@Qenton]

Unless you are from California you won't know what I mean, but that was a very "Huell Howser" type of interview. (That's a fantastic complement by the way) Huell Howser would do interviews of people on PBS, pretty much anyone, and was able to keep it interesting and also for a large audience.

[@gearaxis]

thanks for the video!

[@BenStreeter]

What kind of camera and audio do you use? And on what settings?

[@dananas9131]

Thank you

[@Melthornal]

My mom is a cytohistologist, your comment about histology made me giggle a little. Cytohistologist is a person who practices cytology and histology. Histologists turn samples into viewable slides, and cytologists view the slides. A cytohistologist is basically a self sufficient lab person, they collect the material, turn it into a slide, then view the slide and locate abnormalities.

[@IstasPumaNevada]

That's really cool.

[@youcefdjedi5563]

hi If you have a steel, how can you prepare your sample for TEM examiniations?

[@dbauernf]

Just a quick question Destin - Which lens were you using on your camera for this video? Thanks!

[@galleguilloscastro]

Awesome!

[@LifeOfPabs]

Wow this is amazing... SCIENCE

[@klab3929]

I keep ending up watching all the nerdy stuff, hahah!

[@RadicalAns2]

World's most expensive lunch meat slicer.

[@raxo6513]

8:00 "Nothing in the world looks like it." - "What does it look like?" - "It's brickshaped." sooooo... does it look like a brick? ^^_

[@ChadHHC86]

Not many people know of the name/job Metrology, even though it probably touches every part of their lives.

[@stevejobs5]

sweet!

[@ProFoxMike]

In terms of mind boggling crazy science sh!t that's going on I still think that the FIB beats a microtome by a mile. Imagine you slice, cut and glue a extremly thin sample by bombarding it with heavy Ions. Insanity ! ^^

[@sensorpixel]

Yeah, though I doubt that you can prepare tem lamellas easily out of biological samples with a fib ;)

[@alpine9214]

10:14 "Did you get rid of him?" Jeez, relax, he's not a mass murderer.

[@3doog]

Would be awesome if he actually shows when it's working

[@danheidel]

I was hoping they would have footage of it in operation also. However TEMs are a pain to use. You've got to let the column pump down after sample insertion. (some air gets in through the airlock and has to be removed) Then you have to warm up the filament, ramp up the accelerating voltage, and do a bunch of alignment and aberration corrections. Depending on the scope, getting a sample in for high resolution viewing can take 15 minutes to several hours.

[@NPowell1559]

science.

[@misium]

no actual presentation? not even a resulting image?

[@willlewis77]

Nice and early

[@BigLeprichaun]

300 nanometres? Big? Haha xD

[@SpreadsFire]

GSU!

[@1003k]

Aw yis

[@nivlacker101]

Wish he fired that bad boy up

[@parkyvids5965]

You think that's fancy ... find yourself an Atomic Force Microscope, or better (very rare) a Field Ion Microscope

[@OsricWilliams]

Are you on Subbable? I'd sub!

[@frickbot]

your videos are way to short!

[@Jrodimus]

I swear, Mine Craft is getting way to complex

[@blackberrykathryn100]

So in summary Light microscopes Living specimens x200 mag200nm resolutionlittle distortion no vacuum 400-750 nm wavelengthTransmission Detail within cell 2D0.2nm resolutionx2million magNon living Vacuumnon living specimens some distortion Scanning microscope surface 3D vacuumsome distortion non living x200000 resolution20nm mag0.004nm wavelength

[@GlennSteffy]

STOP SNEEZING !! or everyone gets hurt ......

[@bourkept]

I always see the video title and think that it will be too complicated or boring but then I click and watch the video and it blows my mind.