Liquid Culture: Better, Faster, Stronger

[@CEROtian9279]

Great video! I am actually the export manager for Yuantide Peptone and your video helps us decide to release the consumer version so thank you for that! Peptone is commonly used in fermentation industry acting as a source for protein. Based on our experience from that practice, you can also try to add yeast extract (not yeast) into the mix and see if there's any benefit. Peptone from different material sources can also make a difference (think of it like eating different types of meat), and our client often sees greater benefits from mixing both plant-based and animal-based peptone together (so soy peptone and bone/fish/casein peptone).

[@andrewbarnett2761]

I was thinking about buying your product online. I was wondering do you know if yeast extract would be faster for mycelium? I see you have both products and was wondering.

[@CEROtian9279]

@@andrewbarnett2761 Actually, our yeast extract product is still in transit, but it should be ready in the next few weeks. Meanwhile, you can purchase yeast extract from other companies. Yeast extract is essentially yeast without its outer cell layer, which leads to a higher concentration of nitrogen or protein. We haven't tried it on mycelium, so the main question would be whether the fungi prefer a lower concentration of sugar and a higher concentration of protein from yeast or not. Most bio-pharma plants here have chosen a combination of yeast extract and peptone (because the combination provides a better profile of amino acids), so I hope this provides you with some direction :)

[@TheOffCycle]

Do all of the peptones smell like rot? I’ve used hiveg in my LC but it makes it cloudy and stinky.

[@CEROtian9279]

Hi, in terms of smell yes. Peptone is basically hydrolyzed protein, and those smaller molecule tends to smell rot as you said. However, it shouldn't be cloudy at the concentration this video is using. I assume hiveg is a product by HiMedia so I can't vouch for their products, but our peptone don't typically appear cloudy at such low concentration. @@TheOffCycle

[@davidculver5366]

I am a beginner mushroom farmer in Washington state. I have my first set of bags in the incubation room and I'm almost done getting the fruiting room ready. I just wanted to thank you Andrew for taking so much of your time to make all of your videos for all of us. I am learning so much from you I am truly blessed by your example. I'm only growing mushrooms to eat, and I am stoked to have this new project to keep me busy. This makes retirement a real joy. Thanks again. David

[@soccer2themax]

Your channel is highly underrated. I've been into mycology for months and youtube has only finally suggested your channel. So silly. You are an excellent presenter. Going through your agar stuff now. Thank you for putting these out there

[@dracolusus]

Unfortunately, when you haven't been around long enough, it's hard to tell who to listen to and who not to. Adding motorbike sounds to your video doesn't make it less bullshit. Guy shows no proof, and makes bullshit claims about using a Lazer pointer to look for contams is the dead give away, the guy is living in his own privates confirmation bias lala land. Only way to safety test LC is on agar.

[@livewell453]

@@dracolusus Why are you so agitated friend? He is just sharing practical experience. This video is useful to me.

[@bengreene7563]

Draco I agree about the laser, my guess is he misinterpreted paul stammets when he spoke about using laser particle counters to check sterility of newly made flowhoods

[@Mossycreekmushroomsofficial]

Thanks Paul!

[@Mossycreekmushroomsofficial]

@@dracolusus Lol, never said it shows contamination. I said I use it to help look at the mycelium, if the LC is cloudy, that is usually a dead give away that it's contaminated.

[@jeffboutwellify]

Bro, You have hit the pandora box on LC. I did some Isolate and since using Peptone it has only taken 2 days (unheard of for me/lol) for the Mycelium to grow.. Thank you, thank you, thank you, you have made my life so much more complete!! Mush Luv 🍄💙

[@themushroomlab2645]

I read about peptone in Radical Mycology in one of the recipes, and have also found it to be AMAZING. I find adding peptone to Lion's Mane liquid culture is especially a game changer as Lion's Mane in my experience has a harder time growing in LC than other species. Thanks for the video! I will have to try out that laser tip.

[@tyeejoseph3358]

Comment for the algorithm gods. Thank you for the Educational content. Love from Canada 🇨🇦:)

[@chrisbeman2690]

I am so happy with the results when using peptone. I can't thank you enough for the advice.

[@mnmzuidema]

You are such a blessing to so many. Thank you.

[@thetruthserum2816]

I just realized that you can use air pressure to dispense directly from your LC jars. Connect an air hose to the air exhaust port filter, and "run it in reverse" to push out the liquid...

[@joshuafreeman6720]

I use brewers yeast (nutritional) and it made massive clouds of super fast cultivating culture.

[@chrishandsome5443]

Almost forgot to use the affiliate link..... I'm sure it's a small percent, but I know I would want it. Thanks for the video!

[@julietrotta8429]

Thank you for sharing all your secrets with us!! 🍄❤

[@foltzmusik1]

You’re awesome dude! Love you and your videos. Thank you so much for all you do. SO helpful!!!!!!!!!!

[@brennonmorning9682]

Thank you for sharing! I have Paul's books but haven't worked all the way through them yet. You're inspiring me to get to reading!

[@joshuakidney986]

Love this stuff. Great recommendation

[@CC-ge5fo]

For the Shroom supply premix, do you use the same recipe as your DIY mix? 500ml water, 20 grams premix, 1 gram peptone?

[@cryptohound]

Game changer for slurry ty

[@johngrady1755]

I bought peptone and have been using it for the last couple months. It's funny, you talked about myc forming a mat on the surface on the LC and I almost never had a problem with that just stirring the jars by hand. I started using peptone, and my jars became cloudy, started to look way different to the point I thought they had contams. Also started seeing myc grow vigorous mats on the surface - eventually I realized this was all because of the peptone. I have noticed way faster colonization times from the jars that have peptone and stir bars compared to without them. It's pretty insane.

[@JayJay-bd6ti]

What’s your recipe man?

[@johngrady1755]

@@JayJay-bd6ti I've been experimenting a lot.Traditionally I've used molasses, now I add a small amount of peptone to that. Visually, I add enough to darken the water but keep from making it cloudy. I feel like adding too much sugar can be bad but I don't know about peptone.

[@dracolusus]

You using clones or MS?

[@massagebylmtnicole]

Thanks again for the update and information 😀

[@savagegaming5476]

Is buffered peptone a bad choice? I know it helps with contam but would it effect mycelium growth?

[@SemiPro196]

Just spoke with Brendan with Foggy Dew Fungi in New Castle and we were speaking about how we’re excited to start a new project with 5 Gallon Buckets and cultivate some Oysters species, on a beautiful farm named Laughing Duck Farm. Really excited I have some amazing role models like you and Brendan. Can’t wait to provide food for this blessed home of ours. Good luck and wish you the most success! 🤗

[@traviscowan8323]

New castle in California? I know Brendan! He's awesome!

[@Dee0426]

I purchased some pink oysters from y’all! Can’t wait to grow em

[@dirtdiggerjerry]

I've been putting peptone in my agar. This stuff is awesome!!

[@andrewbarnett2761]

Do you prefer this over yeast extract?

[@stevenrayberry2109]

Hey your videos are awesome!!! I love watching um.. any way I had a question!! I'm new to this... Can I use callagen peptides for peptone??? Peptone is kinda hard to get!!!!

[@karianned3211]

Thanks for the laser tip! 🐬

[@c.odubhlaoich2948]

I got another tip for fast and full growth too. 0.1% agar added to LC mix.

[@Mossycreekmushroomsofficial]

I'll have to try that. Why does it speed up the growth do you think?

[@c.odubhlaoich2948]

Idk for sure, just know it works, I guess it's just easier for mycelium to catch on to agar than just water, but the other good side to it is it makes pooling less of an issue when using LC in a grain jar

[@chrisbeman2690]

Thanks for this tip. I really appreciate it.

[@CaperDano]

Thanks great work 🇨🇦

[@916cali5]

Going too be putting peptone into my very first LCs tomorrow! Very excited! Will give you an update!

[@xvr_demi_trees2937]

Update!

[@phonymex3340]

Update!

[@jeffreyroachii5474]

Gonna try the peptone, thanks brotha!!!!

[@TL-uj7yn]

Is bacteriological Peptone and Soy Peptone the same thing in regards to liquid cultures and substrate nutrient enhancement or is one better than the other for mushroom cultivation?

[@mycologycorner]

I love peptone been using it fr 14 years

[@Jugg215]

i remember when peptone was 14 bucks. its almost 30 now but still worth it

[@iwasbornspecial]

You are the man for this Video !!

[@joshuaimhof4529]

appreciate the recipe!!

[@andrewhsieh3327]

Thanks for sharing Master Andrew !

[@seerifcan]

Hi Andrew! Thank you for the great tip! Would you use peptone in your spawn? I recently started adding a tablespoon of Peptone after my grains are soaked and drained (I guess I can just soak with peptone just like gypsum). I would like to hear your thoughts! Thanks!

[@mycologycorner]

I use to add it to my bulk sub. I got go results off that.

[@iivridesanevii]

@@mycologycorner did you mean good results or no results?

[@mycologycorner]

Yea good results

[@Andrew-co6ye]

@@mycologycorner how much do you add

[@mycologycorner]

@@Andrew-co6ye much 2 tablespoons. But after switching to whole oats I don’t have to supplement my grain spawn. I processed 185 5 pound grain spawn bags last week. Whole oats and hard red wheat.

[@MicahSedillo]

Trying peptone with a lc . Followed your advice and used 1 g . Will follow up on how thick my mycelium gets 😅

[@taranyima9687]

Thanks for the your kindly information. Its useful for new learners 🙏🙏🙏

[@edwinshmedwin]

will soya peptone work?

[@christopherpowell967]

Hi Andrew! Love your videos. I was reading to stay around 4% sugars, but see that with your premix it's a little higher. Have you run into any troubles with a sweeter mix?

[@Mossycreekmushroomsofficial]

I have to reset my cultures more often when running this rich, but that's it really.

[@johnrutherford2326]

You also have to be careful when autoclaving to high or too long, as you can caramelize the sugar. Then it's no good.

[@russellmanweller6694]

I added pepton. It works great. Thanks

[@JoshuaMarshPPG]

This stuff is freaking amazing

[@nicholaspowers4782]

Have you tried adding peptone to bulk substrate? If so any pros or cons?

[@andrewbarnett2761]

I'm about to put a teaspoon in a bag here soon also

[@shannonwilliams7743]

Thank you so much.

[@nachmanedes9460]

Good tip!!

[@drfeelgood1992]

What about using peptone with agar plates?

[@meowmix1569]

No Vegemite or a replacement?

[@exultusgibb2690]

Thanks guy, do you find that this helps with infection, or you do you allay that with sterilization

[@arpitpatni9506]

Thanks for the video. I always wonder how you stir those 200 jars of LC each day? Must take hours even with 10 stirrers!

[@johnwayne420]

Thanks for the tip

[@OakandSpore]

Top quality info!

[@kaezon]

Great info! Thanks 😉

[@organicoceans4459]

Thank you for sharing 🙏🍄🤙

[@zombieprinting2670]

$14 you say for peptone!? I wish! Up here in canada, minimum $40 Plus shipping cost. We get hosed hard here :( Good stuff as always though.

[@smithsmith8119]

how long can you keep liquid culture for?

[@RumoredAtmos]

over a year

[@doya2]

@@RumoredAtmos is it over a year at room temperature? Would it be okay for that long in a room without air-conditioning?

[@RumoredAtmos]

@@doya2 I should have said in a fridge. in room temp man it'll eat all the useable energy in the jar, I don't know how long it lasts in room temp, pretty long but not a year. It is easy to transfer to a new LC just inject 10ml to a new jar of LC.

[@mkstands]

Could you use peptone in a substrate C/V mix for a food to feed mycelium growth? And would it make your mushrooms grow bigger?

[@kimbrigreen950]

Yeah! Would that work?

[@dougiedeathx]

I'm curious of this as well

[@drfeelgood1992]

Anyone try this? Haha

[@didgeridooblue]

I'm thinking since the peptone (a source of nitrogen) works so well maybe a shot of Bragg's liquid aminos (which may be purchased in the grocery store) will be a good substitute.

[@samuel58237]

Did you end up doing this, seems like worth trying.

[@Recifer]

???

[@SparkyHelper]

Thanks!

[@a1moshe]

Thanks for the tips man- does the species of mushroom matter for peptone? are is it usually good for all mycelium

[@Mossycreekmushroomsofficial]

I'm speaking for commonly grown white rot fungus here.

[@on.belief]

Interesting. Do you add peptone to anything else like your grain spawn?

[@tylerallen9856]

I've been doing just a bit of Honey. Would you recommend just adding a gram of peptone to 20grams honey~500ml h2o?

[@sMileHighCity]

TTT Damn you, no I need to order peptone. Been wanting to use it in my agar as I hear its awesome. @mossy do you use it in your agar too? Peace

[@John-gg4pm]

What is the difference between mycological peptone and bacteriological peptone? Why do you use bacteriological?

[@kevinbrown6148]

Did you ever find a good answer, I just got bacteriological peptone in mail now I'm not sure if I should use it.

[@weirdstuffisreal]

So funny I just ordered peptone today before you posted this. Heard in a video about organic cordyceps growing that they would have used peptones but could not because of the organic status. I figured since I am not growing organic I could benefit from it. Plan on using in my multipurpose LC, and in nutrient mix/brown rice for cordyceps.

[@VARocketry]

There are soy based peptones, too. In fact the NIH study found plant based peptone to be superior growth.

[@5cottman]

Do you have to use bacteriological peptone? Or can you use hi veg peptone?

[@tmophoto]

Is there a difference between soy peptone and bacteriological peptone? You also NEED some laser glasses if you are playing around with them like that.

[@JamezGrimm]

I know this video is old, but I really appreciate the tips! I just started collecting strains and making LCs. But I have a question, how do you dispose of your contaminated LC’s?? Do you toss the whole jar? Save the jar?

[@guymontag2948]

Without opening, run the contaminated jar through the pressure cooker to kill the contaminant, then you can open the jar and dispose of the contents and wash the jar normally. The whole culture is definitely lost though, if that's what you meant, barring re-isolating the sample using agar techniques.

[@mike_lives]

Thank you

[@francomushroomtutorialsmus306]

WHat is the best formulation for MALT DEXTROSE??

[@mallissal1895]

Do you use peptone in with the Shroom Supply premix you mentioned in another video?

[@beautycomfortwithmare6602]

I ordered the peptone and when I got it here I found out it’s the “hi-Veg” peptone vegetable protein version. Will it work the same or the animal one is better?

[@hludovikmadoc1351]

Hey, love your video and I'm excited to try this out. I'm new to growing mushrooms but I have some peptone and some spores on the way. Now I know this video is for a liquid culture which is different to just spores but how can I use my peptone to my advantage with just spores?

[@themushroombloke]

Add it to your agar recipe

[@vitopettito1689]

I've gotta say, you should be careful with which lasers you use to check LC!!! A lot of internet green lasers are insanely overpowered and often outright lie on the sticker. A $10 green laser could be easily be 80mW+ which are *powerful enough to vaporize your retina faster than you can blink* should an errant or reflected beam strike your eye in the wrong way. Just make sure you use with extreme caution. Green lasers are actually made by shining IR laser diodes through a crystal that modifies the beam's wavelength. These are not efficient either, so even laser safety goggles rated for just green light could let IR right through, which is invisible but just as dangerous as visible light. That being said if you are careful and only shine it directly into a body like in the video it gets dispersed rapidly. This reduces intensity of the light but is not guaranteed to be eye safe either.

[@sethwalter8751]

Awesome video man keep it up....I hope I get the opportunity of meeting you some day

[@Andrewlamb1]

Are you able to use this recipe for agar?

[@marionunez5262]

Is there a video on how to make agar with peptone????

[@corvo9249]

From what I hear peptone is not allowed in organic mushroom cultivation, can anyone elaborate? I can't find much information on the topic.

[@ahmadrezajavid233]

I used to use pepton in a agar andit cases oiled bacteria

[@dano253]

Could the peptone be used in agar?

[@eazus]

Murashige and Skoog baby

[@imapanderp9713]

your premix is 1 pound lme 4 pounds corn dex, that is 2267 grams of premix. You add 20 grams of premix, and 1 gram of peptone per 500 mil of water. So if you divide your premix (2267) by 20 wich equals 113. So if you add 113 grams of peptone to your 2267 grams of premix would that make it easier than adding 1 gram of peptone every time?

[@Jaba6798]

Have you tested this by chance? I just prepared some LC using his recipe (20 grams @ 1 part malt extract, 4 parts dextrose). There's a ton of sediment. You can't see through the water after stirring. I was thinking about toning it down; either (a) 15g of premix instead of 20g or (b) 1 part LME & 2 parts dextrose. Wondering if you experimented at all since posting this.

[@imapanderp9713]

@@Jaba6798 yes I have, it worked fine but definitely too cloudy for my liking, I bought shroom supply premix it's great, and I use the peptone just for agar...also mushrooms will eat the sediment, but again it will turn really cloudy apon colonization

[@baohuynh3037]

Can u give me information about lme please

[@imapanderp9713]

@@baohuynh3037 what info are you looking for?

[@baohuynh3037]

@@imapanderp9713 what is LME And how to make it

[@aofferle1918]

can you sterilize the peptone with lc? i assume not

[@mukhumor]

Is that mushroom agar powder with soy peptone?

[@rickybradley6177]

Could you add peptone to choir and get a better yield?

[@petrsebesta7017]

Hi, its ok to sterilize pepton?

[@simplyiresistabl]

when do you add the peptone?

[@UnsolvedAz23]

Does this work for agar too?

[@imapanderp9713]

Is it normal for it to get foamy like beer when stirred, if not I need to know what I'm doing wrong

[@Eric-xu1mu]

What kind of needles are you using to get all that chunky mycellium out of there?

[@Anthony69420]

Hmmmmm i wonder if peptone would work in substrate soaks…..?

[@Mossycreekmushroomsofficial]

Some have tried it. I've never seen the after reports.

[@robbyhanlon]

used LME and peptone in some LC and let it set for a few days after PC. when I went to inoculate I noticed a lot of stuff floating around. Is this normal? Doesn't look cloudy, but definitely a lot of stuff in there. I figured the nutrients would remain as sediment.

[@MoCapWoman]

What size syringes do you use to innoculate those monster mycelium?

[@timbryant9346]

can I use the peptone from the health food store like gnc?

[@Algumnomequalquer]

I can substitute peptone for what ? In my country is so expensive

[@sxrry.8206]

I watch this video like twice a year dude (I could write the recipe down but I like the full explanation, Weird I know)

[@singedcarry]

Good video

[@dirtystuff1452]

Anyone know if we can use mung beans to make a spawner? Its easily available for me

[@turgutbelen6374]

Go for it

[@baohuynh3037]

What is lc premix? can you give me information about it so i can buy it please

[@theorbologist5228]

BROTHER YOU ARE AMAZING ,I HAVE LEARNED SO MUCH FROM YOU ,IT IS SO REFRESHING TO HAVE A FRIEND THAT IS HELPING US SUCCEED ,INSTEAD OF TRYING TO DECEIVE US WITH BAD INFO BY LEAVING IMPORTANT INFO OUT.I BOUGHT THE PAUL STAMETS BOOK THAT YOU USE AND PLAN ON ORDERING YOUR LC MIX.TY FOR YOUR FRIENDSHIP. HAVE YOU THOUGHT OF OFFERING ,SPEND A DAY WITH ANDREW ON HIS FARM TO LEARN HOW TO GROW FOR A FEE? YOU COULD EVEN DO GROUPS. JUST A THOUGHT MY FRIEND .

[@codelicious6590]

LC should be agitated every day really?? I have only been doing every two to three days but then again, my needle gets clogged quite a bit when drawing up as well! OOps. Thanks!