No Pour Petri Dish

[@PFLMCTT]

You are awesome! I admire and support the sharing knowledge approach. If everyone were like you the whole world would be in a much better place! Thank you!

[@Mossycreekmushroomsofficial]

Thank you.

[@jett619]

I’m such a sloppy pourer. This is going to change my whole plate game. ✨✨✨

[@Mossycreekmushroomsofficial]

@jett619 wait till my peristaltic pump video comes out!

[@swimstud5151]

I came here after watching one of Yoshi's videos. Searched up the plates then back to KZbin and after two iffy jar vids here I am. EXACTLY what i was looking for. Great video!! Thank you!

[@InstrumentalClassicalMusic]

Where do you find yoshi's videos?

[@swimstud5151]

@@InstrumentalClassicalMusic youtube deleted his channel recently :(

[@jasoncameron590]

You can also drill a hole in the lid and add an injector port to them. Great for starting a LC.

[@charlesdunlap7821]

I like how them containers are sealed you know what’s in side is contam free I’ve been doing the about the same thing using a short deli container I get from local grocery store or Menards make sure there’s #5 on bottom container poke hole in lid cover with 20mm filter patch or micropore tape and go at it

[@arabiclearner711]

So we should let it build a vacuum when cooking? What about leaving the cap loose slightly then tightening after it sets so no suction when opening again? I was expecting you to say leave the caps loose like liquid culture and was surprised to see the vacuum so that’s why I ask.

[@Mossycreekmushroomsofficial]

I don't leave my LC lids loose, and no I don't let these stay loose either. As they cool they can suck in contaminates.

[@karmaTrinlema]

How do you prevent them from blowing in the autoclave??

[@Queefy]

I’ve had good success with 4oz mason jars doing this.

[@MissBlackMetal]

I immediately went to look for those petri dishes as soon as I saw how large they were in your hand, and that they're PP 😂 I scoured the website you linked and found a couple other items that mycologists might find useful, but rather than petri dishes these would be useful as culture slants: item# 2924B01CLR and item# 314940 (the latter of which I imagine will be the better option, but I'm buying both to see). They're both clear PP tubes with hinged snap-on lids. I'm hoping that with Parafilm, the snap-on hinged lid design won't pose any issues. These are all cheap as dirt -- *MUCH* cheaper than proper lab supplies... so I'm happy to try these out 😁 Thanks for the video, as always! 🤘🏻

[@SnufflePig]

Great vid man, thanks for passing on the knowledge!

[@user-cc1ws1ud8k]

When you go to pressure cook them, do you put them in a jar or a bag? Or do you just put them into the PC as is?

[@JoshuaMarshPPG]

Holy crap, where was this when I had Rocket City mushrooms. By the way I'll still watch your videos Andrew, I miss growing mushrooms. Keep up the awesome work Andrew and MCM.

[@Mossycreekmushroomsofficial]

I'm glad to see you still floating around. See what I did there?

[@JoshuaMarshPPG]

@@Mossycreekmushroomsofficial I'll be up that way soon to fly. I'll stop by. Will be spending the weekend up there

[@Mossycreekmushroomsofficial]

@@JoshuaMarshPPG Please do stop in! I've seen some guys out here sailing over the shop lately.

[@JoshuaMarshPPG]

@@Mossycreekmushroomsofficial you got a school close to y'all. Pinnacle Paramotor.

[@NoMaDv2]

Is the toothpick necessary if not for long term storage? I thought it was for nutrients after the agar is used up, thanks.

[@Max-on1hk]

Indeed, the toothpick is for long term storage usually in slants as mycelium will colonize the wood and outlasts agar from what I've read. I guess this could be a good longterm agar dish solution??? But why

[@MissBlackMetal]

Soooo what's the toothpick in each dish for??? He said "you can use a toothpick or sawdust", but the only thing I know sawdust is used for is as a fruiting substrate, not in agar petri dishes... Can anyone please elaborate? I'd appreciate it.

[@macmckulis7440]

That is just incredible.

[@paranoidmmm]

Amazing, was going to buy glass ones, Going to try those instead.

[@Mossycreekmushroomsofficial]

Easier to work with than glass.

[@jeffmcgraw1577]

Thanks for sharing your knowledge on these instructional videos! Super helpful. A couple quick questions... I wanted to try out the Petri Dish replacements you recommended but the page linked to Berlin Packaging isn't working for me (it turns white and fails to render properly even when I navigate through their menu UI). You mentioned having other sources for these dishes. Could you share an alternate link? Also how do these jars allow gas exchange?

[@brainyquizchallange]

I believe PastyWhite started this years ago on the Shroomery. It's easier to just mix, pour, clave.

[@Mossycreekmushroomsofficial]

You can certainly do it that way. If you want to make up only one recipe I think that's not a terrible way to go.

[@brainyquizchallange]

@@Mossycreekmushroomsofficial Many paths to the same destination. Also, check out us plastics for a alternate source of containers.

[@Mossycreekmushroomsofficial]

@@brainyquizchallange Thanks!

[@killerkennyas]

Yoshi is good peeps..thats awesome

[@coloradodirtbike5930]

He knows his stuff for actives that's for sure

[@charlesdennis6231]

Thanks great stuff!

[@RockSolidSilverStacker]

Just found your channel. Great video. Imma give this tech a try

[@KitchenMycology]

Great Video! I've been using those same cups for a while and Love them! Now, I mix a batch of Agar and pour all the cups after. Any reason you mix them in each individual cup? Thanks!

[@KitchenMycology]

Thanks! Yeah, I use a Still Air Box, so I don't like to pour them in the SAB. I like that after the PC is done, I can just tighten the lids and I'm done.

[@SparkyHelper]

YAY! New Vid!

[@TheCragg777]

I put the food coloring in the water. It seems to be more consistent across multiple pours.

[@MrOiam]

I like the dishes, but going to try with a micropose filter on top to eliminate vacuum issues

[@Jaba6798]

Andrew w/another master tip.

[@arabiclearner711]

It seems so tedious to make those small measurements and inject water into each dish separately. Would it not be easier to just make up a batch, mix once and then pour a little in each dish then pc them?

[@Mossycreekmushroomsofficial]

You can certainly do that if you so desire, but I don't find that easier especially when I'm making up dishes of different recipes in the same batch.

[@xj11w2c7]

Mictopose makes PP 5 autoclavable 90 mm x 15 mm Petri dishes that you can buy in cases of 500 or smaller amounts

[@rhb30001]

They are not screw on though … parafilm is needed after pouring

[@TR-sj9fp]

Love your channel!

[@detritic]

I swear everyone one of us must own at least one of those silver scales with the blue lcd

[@Mossycreekmushroomsofficial]

You know it!

[@funkonabarbie]

Have you experimented with putting a syringe filter on the lids for air exchange? Or is it not necessary?

[@nastydave6334]

I tried this. Didn't go well for me. I used the ones from Amazon before he changed the link. My problem was how deformed they got in the pc. The top and bottom are so concave that the agar is touching the lid in many cases. Also a lot of them leaked out and they were sucking in air as they cooled. I'll still try to use the ones that look OK but I'm not a fan of how it came out for me. Things that I could have done to mitigate this: -Put a layer of quart jars on the bottom of PC. I noticed the bottom layer of the no pour dishes were the most deformed. -not close the lids as tight on the jars before pc. Should they be tight? - I released the pressure after it was down to 5 psi. Probably should have just let it cool in the pc. This could even have been my biggest mistake. Are the toothpicks really needed? When the bottom of the jar warps the toothpick comes out of the agar. I will try again with the above tweaks, the new jars, and update. I'm really attracted to being able to re use these, also being able to sterilize them and putting an injection port seems really smart.

[@ZeginMakesMusic]

I love pouring agar into petri dishes. It is part of my entire ritual. In my opinion, to make any of this easier is a disservice to the mycelium. The process is meant to teach patience, something that most Americans lack.

[@rabidrabbitsmokingneonlights]

So I've scrolled through vid like 10x and can't seem to find the recipe amounts for these cups but I think I remember them 1/4 tsp agar 1/4 tsp lme and 20ml water Am I remembering correctly?

[@xsavior369]

I'm assuming the toothpick is for the wood lovers? Is there something I would supplement for dung lovers?

[@bangdingow1414]

Hmm....is that a rhetorical question? Seems kinda obvious wouldn't you think? Almost any type of manure product.

[@Mossycreekmushroomsofficial]

A rabbit pellet? Black Kow from Lowes? I'm not entirely sure.

[@jesseakc]

No parafilm I'm sold!!!

[@Mossycreekmushroomsofficial]

Down with Parafilm!

[@austinfisheroutdoors]

@@Mossycreekmushroomsofficial So are you not doing any kind of gas exchange with these at all? Nothing is discussed about it.

[@john-smith.]

Pinks are always in....or out ;)

[@drewgraham2294]

Where did you get the screwtop containers/dishes from?

[@austinfisheroutdoors]

Here is a question: Petri dishes for mycology typically have vents for gas exchange. These twist top containers do not (as far as I can tell). Isn't that a substantial con?

[@sporemuse]

Thanks for this video! i love no pour techniques and yours is pretty cool. i currently use larger reusable screw on lid dishes, but i want to migrate to those smaller ones. Have you found any of those smaller ones that has more transparent plastic?

[@JuanTiller]

Could this same technique be used for regular petri dishes that have been sealed with pore tape?

[@Vinntiss]

Would theses dishes also work as a slant, since it seems air tight? Thanks for showing us this, going to get myself few right now lol.

[@Mossycreekmushroomsofficial]

I'm thinking you could use them like that, but I would want long term testing done first before I switched from slants. I also prefer storing the slant shape over the dish.

[@prod.lilloco]

Does the fact you can autoclave them mean they will be fine pressure cooking as well?

[@Mossycreekmushroomsofficial]

Yes.

[@cosput]

Why add the toothpick?

[@cosput]

@@IlIlllIIllII Cool, I guess I can swap in a hardwood pellet then 🍄

[@Mossycreekmushroomsofficial]

@@cosput Yep you can. I thought I said that in the video, maybe I forgot to add that part.

[@peters7211]

Could you use a microppose filter on the lid to stop the vacuum?

[@Mossycreekmushroomsofficial]

You could, but filters fail and agar dries out.

[@JinTalzeus]

How do you keep it ventilated after inoculation? Do you keep the cap loosely covered? Do you still use parafilm with this method?

[@Mossycreekmushroomsofficial]

No parafilm, no need to vent. Lid is not kept loose.

[@JinTalzeus]

@@Mossycreekmushroomsofficial can you expand on this? Why it doesn't need to vent. I thought mushrooms need to breath in order to grow.

[@Mossycreekmushroomsofficial]

@@JinTalzeus There is some air in the container once you've worked with it in front of the flow hood, but mushrooms do not need as much air exchange as we often think unless we want rapid growth of a lot of biomass. Interestingly enough as well, many fungi can pull oxygen from water molecules and use that for their metabolic needs. It's slow, but how they often grow deep in a log without access to fresh air from outside the log.

[@seanm191]

Are petri dishes suddenly not clavable for some reason? The ONLY difference I'm seeing is no tape.

[@Mossycreekmushroomsofficial]

plastic, disposable dishes are indeed not autoclavable. The big difference is in no pour and no tape.

[@Pothos969]

I followed your directions. How did you avoid condensation?

[@jordynpooler2406]

What time on high temp would you recommend in the instant pot?

[@FeatherzMcGraw]

What's the contam rate like? When opening, the vacuum could pull contaminates in? I guess not so much of a problem if you got a flow hood.

[@scoddiboi]

I assume you could leave the lid cracked slightly and let them cool in the pressure cooker overnight, when you release the seal on the pressure cooker make sure to then tighten all the lids. The inside of the PC is sterile until you pop the lid, so letting them cool in there should be fine contam wise even with the lid cracked

[@harleythompson7954]

Why is it called no pour when you pour it?

[@qpir26]

Does it need to be gel food coloring?

[@HelloHi-qm3ii]

Ok you made a long video, why not mix all ingredients with water and pour half of the containers and autoclave them ?

[@rubenbarela]

I just heard of him today and requested him

[@ruthe6017]

Can you not use jelly?

[@ronsmith9992]

What is the purpose of the toothpick?

[@rockyrivermushrooms529]

how many petri dishes are you making per week?

[@Mossycreekmushroomsofficial]

That changes week from week. Could be anywhere from none to hundreds.

[@anonharingenamn]

Wish I could find something like this in Europe. I'm sure after a lot of googling I can. Anyone in Europe using something like this?

[@silverleapers]

Sterilization canisters and prepour in glass is what we do.

[@JinTalzeus]

What is this sterilization canister you're using?

[@silverleapers]

@@JinTalzeus google "sterilization canister" and you should get an image.

[@Mossycreekmushroomsofficial]

I've done that a fair amount as well, not a bad way to go. Used to sterilize a lot of batches in media bottles and then melt and pour after the fact.

[@LoreCraftForge]

Where’d you get your metal racks from?

[@bangdingow1414]

Tarrison is probably the best but holy $$$. The ones you buy at home depot or lowes are a lot cheaper and are pretty decent.

[@LoreCraftForge]

@@bangdingow1414 thank you!

[@asamusicdude]

Been using these for years but they don't have them on Amazon anymore. In Etsy they're 30 bucks man

[@Mossycreekmushroomsofficial]

30 bucks for how many?

[@asamusicdude]

@@Mossycreekmushroomsofficial for 10 of them

[@asamusicdude]

@@Mossycreekmushroomsofficial do you think it's worth it? I do like them better than the Gladware mini rounds

[@kristinesutton846]

What is urisum?

[@Mossycreekmushroomsofficial]

Hericium, it's the genus that Lions mane and Comb Tooth are in.

[@chuckheppner4384]

♻ Righteous! 👍🏻

[@john-smith.]

Andrew if your eating rabbit......just know they will be waiting for you when you go down that rabbit hole.

[@Mossycreekmushroomsofficial]

I'm planning on it!

[@puffitaround993]

i toock it a step further added a syringe filter plus a self healing injection port

[@adambiggums5473]

Lol yoshi isn't in the legit end soooooo yeah good luck also Mr alien is the originator

[@Mossycreekmushroomsofficial]

If you mean that he isn't in the "legal end", I completely understand that and have no issue with that. I can only give credit as things come to me. I don't know Mr. Alien but I'll look him up. Credit only can be announced in the form of lineage.

[@Mossycreekmushroomsofficial]

And by have no issue with that, I mean in essence, "All truth belongs to the saints."

[@whatthefunction9140]

I want to get into tissue culture

[@Rustmonger]

As long as you are clean and sterile it is easy peasy. Tissue onto agar or into liquid culture is just amazing. It works so fast.

[@Mossycreekmushroomsofficial]

Keith is not wrong, it takes practice but it's not bad.

[@bareknuckle7106]

man that music loop is bad

[@john-smith.]

Yeah it kinda sucks....i got thru it, but you know how some people are.

[@Mossycreekmushroomsofficial]

@@john-smith. I sure do know how they can be.

[@Mossycreekmushroomsofficial]

Give me something I can work with. Too loud, Too repetitive, Not your style, or whatever. There isn't much I can do with, "that's bad."

[@johnstirzaker6832]

​@@Mossycreekmushroomsofficial it sounds like factory noise.

[@synthpulse1]

Industrial!

[@terryrogers8503]

K cups or ketchup cups are cheaper than this and just as effective. At least you can get the lid off. Can you make videos with teks you came up with, instead of using other peoples. Very sad after all these years you make very little improvements on your own to share.

[@oscarsmith9992]

k cups aren't reusable like these are and are NOT just as effective. And who cares if he came up with the tek. if it works it works. Your ridiculous

[@Mossycreekmushroomsofficial]

Hey look folks, it's Terry! Back with something negative to say! How unlike him, either way, thanks for driving engagement for the algorithm my dude!

[@JoeMushrumski]

When you clave those, are you tightening down the lid with the vacuum or 1/4 turn back?

[@Mossycreekmushroomsofficial]

I've just been tightening them.

[@JoeMushrumski]

@@Mossycreekmushroomsofficial The vacuum after cooking is a b*tch to open. Lol, I now have 4 that won't budge.

[@benink5690]

Whats the topthpick for?

[@jett619]

That’s my question as well. 🤔

[@MagicinalExplorer]

I love those dishes they work well shout out to @mendomyco peace