Рет қаралды 269
In this video tutorial, we will demonstrate how IsoQuant can be used to identify and quantify isoforms from long-read data in OmicsBox.
LRGASP challenges: Systematic assessment of long-read RNA-seq methods for transcript identification and quantification | bioRxiv www.biorxiv.org/content/10.11...
IsoQuant repository: ablab/IsoQuant: Transcript discovery and quantification with long RNA reads (Nanopores and PacBio) github.com/ablab/IsoQuant
0:00 - Intro
0:20 - Why perform isoform identification
1:14 - Why use IsoQuant
1:38 - Data set
1:57 - OmicsBox demonstration
2:13 - Run IsoQuant reference-guided
4:00 - IsoQuant reference-guided results
4:58 - Run IsoQuant reference-free
5:43 - IsoQuant reference-free results
6:08 - Run IsoQuant for reference quantification
7:19 - IsoQuant reference quantification results
7:35 - Conclusions
Download OmicsBox for Free: www.biobam.com/trial/