Mapping restriction sites on Plasmid DNA_ tutorial 2

How to map restriction sites on plasmid DNA.

[@alallyshaalkhadi6735]

a very great job

[@freshlycleanedhands]

This was very helpful!

[@cherobinn]

this was very helpful thank you so much!!

[@savindul.w.7428]

Thanks a bunch 🌟🌟

[@arjyamazumdar4508]

you didnt show the place where the 1550 and 1450 fragment shoud have been added to the plasmid....you made two plasmid on with only Enzyme 1 and 2. can you please add it to the plasmid

[@sarahedoh2974]

Thank you so much... This video helped me solve a problem I've been battling with... Wonderful Job🎉

[@4EvaBio]

So glad I could help

[@saadafares3894]

Wow thanks 👍🏻👍🏻

[@4EvaBio]

You’re welcome 😊

[@geethika4852]

What if E2 also gives two fragments. How can we do the mapping then?

[@chimezieokafor5117]

you did not add enzyme 3 to the final map, is there any reason for that?

[@4EvaBio]

Not sure what you mean. Enzyme 3 is definitely there :)

[@katherinerosa7]

You didn’t show how to find the specific location of the restriction enzymes

[@4EvaBio]

Awww.. looks like you missed it. Ok, remember a specific restriction enzyme only cuts at a specific DNA sequence. So mix your DNA with that enzyme, and you will find the cut- the location. If you have more than one enzyme, you can build "a map" of restriction sites- cuts. Here's an analogy: take a circular string, cut it into different length pieces, then try re-assembling the same circle... like a puzzle. The location where the pieces meet- is the location where the specific enzyme cuts.