Restriction Enzymes and Recombinant DNA

[@tshepangkhahlu4076]

This is the best biotechnoogy lecture series I have ever watched.

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I love you, man. Like you’re saving me because your ten minutes videos are equal to a 2hr class. Than you.

[@AKLECTURES]

Happy to hear it! :)

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You really are a good teacher. Thanks for doing this!

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I wish I discovered your lectures earlier! Very good stuff!

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And I see this 4 years later

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​@@tejasvisakhamuri1380 And I see this 4 years later

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Andrey, really appreciate your videos; specifically writing down the sequences and showing with different colors really makes it easy to understand. Thank you very much!

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DONATED !!! Today I passed the final exam successfully in the subject of “ Horizontal Gene Transfer “ in medical school because of your video lectures !!! I can’t find the words to thank you honestly!!! Without you , I would have been lost or failed!!!

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+nick smith thanks for giving it a shot! :)

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*skeptical

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BEST explanation with details EVER, you really understand what your are explaining. Thank you, we needed you.

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The same I also didn't understand my lecturer i am from Kenya 😂😊

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Still amazed!

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I just love your way of teaching and especially the content!

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I'm seriously mind blown now..like all these were really confusing but after hearing this lecture everything connects and makes sense..Thank you so much

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just 2 minutes into ur lecture I had to pause the video and Comment on how informative this video was !!! This is amazing! I loved it ! Thank you so much, & keep doing what you do !

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very help fully

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Good lecture

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how is the coronavirus

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Lecture is 10/10. Legendary teaching.

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Greetings from Brazil! Your lessons are really very good! It seems to be easier after that, and you have a perfect "blackboard". Thanks a lot!

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João Carnero Guedes Thank you! glad to see they're helping you! :)

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So easy to follow and understand. Thanks for taking the time.

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Ur excellent i wish u were my biology teacher

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Omar Abouraya Thanks Omar! :)

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same here 😔

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why can't we use PCR for amplification?

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Wooow....it is explained so clearly and the explanatory diagrams are best .Great teaching sir..Thanks!

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I hate genetics but you are the best lecturer i have ever heard. Loved and understood each of your videos 😀 Thank you!

[@thuthuyle5876]

What should I do if the new plasmid does not contain the DNA fragments of interest ? T-T

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Everytime I search for my topic you come up...What DON'T you know P.s you're a great teacher!

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I adore the way he lectures

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bushrahussein thanks! :)

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OMG you are the best. brilliant way of explaining hard subjects.

[@makulmahato8663]

The ligase can rejoin the original DNA strands as well as it can form recombinants, which can be identified and separated by some techniques. To avoid self ligation of the DNA molecules, an enzyme called Alkaline phosphatase can be added to the DNA fragments before ligation. This enzyme removes the 5' phosphates from the DNA segments. Now the DNA fragments can only attach through h- bonding between the sticky ends of the DNA molecules. Inside the bacterial cell The DNA repair systems join the single stranded nicks. The recombinants can be purified later.

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You are amazing! Thank you so much @AK LECTURES Keep up the good work!

[@AR-nj8co]

Thanks for this video! It was really informative but also simple to understand, you included a lot of information that put all of the little parts into perspective.

[@deeep124]

Which enzyme cleaves hydrogen bonds when the restrictions enzyme cleaves phosphodiester bond , to separate the DNA segment ?

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I love your lectures. Glad I found these. I do recommend using a different colored t-shirt though. White on white is jarring to my optical nerves.

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You're just infinitely great. Thank you a lot.

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Love your lectures...thank you.

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This make sooooo much more sense!! Thanks man 👍🏼👍🏼

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Hello AK...I'm back and as usual your videos give me a positive energy to go through my midterms 😄 I can't say how much do I love you ❤️

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Saviour, thank you!

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You ARE my professor!

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THIS IS SO HELPFUL

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You are awesome really, thank you for your lectures

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Very interesting, The lecture is well digested, thank you.

[@MartianSA]

Great videos! Your teaching style is exceptional! They definitely clear up many of my questions in my readings. However, I did have one question pertaining to this. When you say that DNA ligase is added to combine the two sequences of DNA and form the recombinant DNA, how do you make sure that DNA ligase doesn't just form the two original circular forms of DNA?

[@isiherrera5408]

Shayan Alam i have the same question

[@natureetsimplicite3358]

I think the DNA Ligase will join the DNA through reforming the bond between the 5′-PO4 coming from the insert and the 3′-OH of the plasmd

[@frenlyneybur7544]

I am probably too late to reply but... A phosphatase comes to take dephosphorylate the plasmid (so it doesn't reanneal) When the gene if interest comes along, it binds to the plasmid by its phosphates.

[@tinotendakunaka6807]

thank you so much...you are such a good teacher.

[@Dira091998]

thank you so much for this video! i have a genetic recombination midterm exam tomorrow. This video really helped me :) wish me luck!

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What amazing explanation dude Thanks alot.

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Thank you soo much for the explanation🌻🌻

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Big thank you from France! :D

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I fall in love with everything in ur video :) god bless u💕

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Tabarak Ali is it clear to you

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Great teaching. Thanks very much.

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Amazing video, great explanation. I don't understand why anyone would dislike this lol. Thanks!

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Pretty detailed and very informative. Thanks a mil

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I can’t thank you enough man!!

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Very clear lectures thnx ...AK LECTURE

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This was so helpful, and very well explain, thank you so much you saved me from getting a bad grade on my exam

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awesme teacher.... well understood

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Amazing teacher !!

[@satbiology9890]

thanks a lot very well understanding..but I have a question, we say that bacteria use these restriction enzymes to digest the viral dna when infected by them and before doing that uses methyl transferase enzyme to protect it's dna from these restriction enzymes...my question is, are these enzymes always present in bacterial cell or they will be produced up on infection??? is there any mechanism for that ?? best regards

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Mustafa Fahmi انا كنت هسقط اقسم بالله لولا الفيديوهات بتاعته !!! رهيب نجحتي فسنه اولي و سنه تانيه طب فمواد البيوكمستري و المولوكيلور بيولوجي و الهوريزونتال جين ترانسفير !!! عبقري !!! يستحق فلوس الدراسه مش الجامعه و الله

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I freakin love you. Don't stop makin videos. Cause i dont wanna stop lovin u

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thanks! i wont :) also your video with the two cats is hilarious.

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Great job.

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Awesome job. keep it up!

[@khandakershadia6462]

After adding the enzyme ligase why don't the sticky ends of the same circular DNA reunite?

[@michaelfedorovsky7944]

Had the same question

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Thanks for the videos AK

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+Viktor Holicek you're welcome Viktor, thanks for watching

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thank you very much doctor you are the best

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Dude this is really good. Thx man.

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T Math thanks! :-)

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great. Thank you. Really appreciate it, it is very helpful.

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Can you add a picture of the white board linked in the comments? Thanks by the way, I would have failed biochem without your video series.

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THANKS FROM NZ

[@JaLLaM86]

Very nice video, thank you :) I think there is a mistake though. The 5' and 3' sticky ends do not match in the upper left drawing and lower left drawing.