Maxam and Gilbert Method of DNA Sequencing

[@DB-kv3wu]

Best lecture with best communication

[@afrasiabwazir8751]

sir your hard work and amazing lecture make me understood and interested ..thank you very much sir from Pakistan (Peshawar).

[@biovisionacademy3203]

Thanku very much dear. Thanks for your apprication. Respect and honour from India also.

[@Aniketpandey3495]

Very nice explanation sir.Your teaching skills are awesome🎉.

[@HamzaKhan-ct2oo]

Love from pakistan(peshawar) best explanations giving massage at pashto wah sir derr zbr10

[@biovisionacademy3203]

Very very Thanks Hamza. We Love pakistan also. Thanks for this motivation. N please subscribe to our channel

[@Wasim..]

der wran ye alaka

[@farooqahmed8537]

Sm sha hamza

[@BioHUb-be4bs]

Awesome teaching skill with clear cut concept ☺️ Thankyou so much sir 🙏🙏🙏

[@upadeshpattnaik7093]

VERY GOOD EXPLANATION SIR GOT CLARIFIED IN THE VERY FIRST TIME.KEEP MAKING MORE VIDEOS LIKE THIS

[@bio321]

Sir your teaching method is very fantastic and you made me clear at this topic. Thank you very much sir. From:Pakistan

[@biovisionacademy3203]

Thanks Gulzar. Please subscribe to our channel

[@CuteBabyManvi]

Effective explanation, thank You Sir

[@annaaditi126]

The content is good and explained in a clear concise manner. The only problem is the constant focusing and de focusing of camera is very disturbing and strains the eyes too much.

[@amirktk1626]

Love u and your LIC from the core of heart sir from pakistan(karak)

[@syedsalman3987]

Love from pakistan 💗 Indian teachers are always appreciable.

[@biovisionacademy3203]

Thanks sir. Please subscribe to our channel

[@briardamon9291]

instablaster.

[@farooqahmed8537]

Amazing explanation dear sir love from peshawar pakistan

[@akkatutorial8529]

Best lecture , Thanks a lot sir

[@jyotiyadav5265]

Clear explanation thank you sir 🙏

[@gargimukherjee6841]

It has been very helpful, sir, thank you!

[@biovisionacademy3203]

Thanks Gargi. Please subscribe to our channel

[@dr.rashmil1143]

Hello sir, very nice explanation. One clarification please, how do you got know about first nucleotide, what sequence does your first band contains. Thank you sir

[@mahimakawa1234]

Thank you so much... It was awesome explanation 😊

[@kajalbhati4065]

It's really helpful thnku sir

[@biovisionacademy3203]

Thanks kaajal

[@PariShil]

Omg that's really helpfull thanku so much sir it is really in detail and u taught in easier way😊😊

[@biovisionacademy3203]

Thanks. Please subscribe to our channel

[@muhammadusamakhan7636]

Very crystal clear but I'm confused at 16:37 Glycosidic bond or phosphodiester bond

[@fatimaashfaq3546]

Thank you so much sir.... Very useful

[@biovisionacademy3203]

Thanks Fatima. Please subscribe to our channel

[@hirachaudhry6293]

Woww very usefull genius teacher😍

[@biovisionacademy3203]

Thanks hira. Please subscribe to our channel

[@KaushikRachna.2024]

Believe me guys this video is best 💯💯💯💯💯💯💯💯💯💯💯💯💯

[@amitsharma-tj4nm]

Thanks a lot for nice explanation It is very helpful

[@zahirshah2172]

Well done sir 👍 thank you sir you understand really well

[@khangulabsa6360]

Explanation is excellent but sir you mentioned in second test tube add DMS that is incorrect.In second test tube add formic acid and piperidine only not DMS

[@bumboschlumbo391]

Thank you for the Video Sir. I finally got it.

[@sagarsoni6626]

Explain in easy way all point clear every steps

[@amitkumarswain640]

One question sir that why we use the endonuclease enzyme to cut the DNA to two part a small and one large

[@sofiastar9197]

excellent explaination

[@biovisionacademy3203]

Thanks Sofia. Please subscribe to our channel

[@himashreepatowary9053]

A very good explanation.... 😊

[@biovisionacademy3203]

Thanks dear himashree. Please subscribe to our channel

[@pramodsingh7348]

Great effort sir

[@farmanali9203]

Really appreciated and satasified vidue about gilbert method

[@biovisionacademy3203]

Thanks. Please subscribe to our channel

[@ahmedmiskeen8733]

After cutting with restrictions enzymes Both long and short fragments have one phosphate not the long strand have two r p32

[@nikitag7207]

Great sir 👌

[@rifnakv6292]

Good explanation

[@biovisionacademy3203]

Thanks rifna. Please subscribe to our channel

[@tharikidapp9605]

How Will we know first base is thymine...if we remove phosphate from thymine it is not radioactive..it will not show in autoradiography...

[@biovisionacademy3203]

Dear watch video carefully. You will find your answer. Thanks please subscribe to our channel

[@DB-kv3wu]

Dereje Bekele 1 second ago Best lecture with the best communication, but I couldn't understand making the band using a line on the video starting from the 27th minute.

[@yanintmadona288]

wow wow the most Brilliant one.from ethiopia

[@Biotechie_Concepts]

You are a life saver for me 🥰🥰

[@iagoaraujo7473]

what is the purpose of cutting the DNA with the restriction enzyme? i don't understand it

[@biovisionacademy3203]

Short DNA 🧬 gives b better results with high accuracy

[@bunnycitizen4753]

amazing lecture... it made me to subscribe ur channel

[@taruna7941]

Thx a lot sir... very well explained

[@biovisionacademy3203]

Thanks Taruna. Please subscribe to our channel

[@realislam4491]

Thought best compared to whole utube teacher

[@biovisionacademy3203]

Thanku very much ehsaas. Good to know that my video help you to understand about DNA sequencing. Please subscribe to our channel

[@arpitkhanna3039]

Sir its a very informative video

[@sanjuvegad9290]

Nice explanation 👌

[@muhammadasifali1221]

Love u sir from Pakistan

[@asthasingh5709]

Thnku so much...plz mak more videos

[@biovisionacademy3203]

Thanks. Please subscribe to our channel

[@aqibamin6512]

Sir i think there is no need of cutting DNA with restriction enzymes before denaturation of DNA bcoz in denaturation the DNA will itself divided into two pairs in which both will have radioactive 32P .

[@iqraakram5187]

Sir i have a question Sir why not terminating T that have a bond with phosphate break in C +T test tube????

[@venkatesanmunusamy7319]

Thanks. Great explanation.

[@hayakhan5353]

Soooooo helpful👍👍👍

[@dakshitamann310]

Well explained sir

[@shuvajitdas5021]

great sir, i have only one doubt that are there exists any alternative of hydrazine or other breaking molecule? we use it because it is best for pupose right?

[@biovisionacademy3203]

Yes u r rt. Shuvajit

[@biovisionacademy3203]

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[@shuvajitdas5021]

Thank you sir, can you give an example of other alternative breaking molecule that do the same splicing like hydrazine

[@saminakhan1308]

Toooo much informative thank u so much

[@biovisionacademy3203]

Thanks. Plz do subscribe to our channel

[@ankitayadav1123]

Thank you for this explanation 😀

[@biovisionacademy3203]

Thanks Anamika. Please subscribe to our channel

[@arshadghaffar6458]

You said that the chemicals cleaves before each base. So when the first T is cleaved at 5 prime end only phosphate is left so how we will read this from the gel?

[@KaushikRachna.2024]

Same doubt

[@nishasharma6202]

Thanku sir

[@biovisionacademy3203]

Thanks Nisha please subscribe to our channel

[@rimaakter2116]

I like thik video...thanks

[@nilaysarbas211]

thank u teacher

[@navadeepdorai2918]

Thank you

[@saurabhkant2738]

Thank you sir🙏🙏🙏👏👏🙌

[@biovisionacademy3203]

Thanks Saurabh. Please subscribe to our channel

[@ladjalhanane3860]

thank you sir

[@archanaadhikari8513]

Thank you so much sir .... this video help me a lot :)

[@Shabbir2749]

Thanks sir 🙏

[@rachana5036]

Tq sir

[@sushmayadav3087]

Understood all the points except the electrophoresis part🤔

[@biovisionacademy3203]

I will suggest u watch the video again. U will understand all the points. Please subscribe to our channel

[@SegunBadewaMayprilla]

Thank You!

[@biovisionacademy3203]

Please subscribe to my channel

[@mdabuusuf7034]

May Allah bless 🙏 you

[@TriggerJigar]

Thanks alot sir

[@anikethpulitzer6333]

Sir , where did you get this info from ? The name of the book please . Thank you

[@biovisionacademy3203]

First' of all i would like to thank you for watching my channel. You can get information about maxam and Gilbert method by referring the books like lodish , Wilson and Walker, Smith and Smith, Upadhyay Upadhyay and Nath lehninger lodish etc

[@anikethpulitzer6333]

@@biovisionacademy3203 Thank you sir

[@biovisionacademy3203]

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[@anikethpulitzer6333]

And sir , what is the role of Formic acid and NaCl in the second step of sequencing?

[@moosiin6247]

the background noises with cars :D

[@biovisionacademy3203]

Hahahahhaahh. Moosin please subscribe to our channel

[@prafulkalwade6701]

How the redioactive phosphate group came at 5 prime end of larger strand after restrictions enzymes cleaves dna

[@prafulkalwade6701]

Reply me fast

[@muhammadsarfraz5159]

ThankSssss sir g

[@Bionector1017]

sir ap konsey college me paddatey ho me phd krlunga apkey under

[@serajnajar2776]

Ok students this is our dna Students

[@biovisionacademy3203]

Hahahahahaha. Ok seraj I got it. Thanks. Please subscribe to our channel

[@mohithinsu4017]

Good

[@biovisionacademy3203]

Thanks. Please subscribe to our channel

[@arshadghaffar6458]

What's the function of radioactive phosphorus in this process?

[@daminikhawaskar6033]

After gel electrophoresis this gel is placed over radioactive film where these radioactive labelled products will emit light

[@arshadghaffar6458]

@@daminikhawaskar6033 thanks dear for your answer. Radioactive phosphorus is added to the 5' end so it will be present in every DNA strand either smaller or larger. So I'm not clear that what it's function?

[@souvikhajra4213]

Make more video sir

[@ajaydhillonajaydhillon8590]

Caz understanding

[@ajaydhillonajaydhillon8590]

Good

[@bioatbench350R]

Sir if phosphate bond is cleaved from 5' end thn how autoradiography will detect the band bcz it will be lacking radio active isotopes

[@kj4571]

nice explanation