Dideoxy DNA Sequencing - Sanger method

[@BaaYo]

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[@nirupamroy3941]

"It doesnt matter your knowedge if you cant teach it to a layman" -Albert Einstein. Thank you, this video helped me while i was thinking about going for a Hi-Di Sanger sequencing for my sample.

[@BaaYo]

Thank you Nirupam. :)

[@vaisakhrajeev3018]

Your Chromatography tutorial helped me a lot in the semester exam,Thank you 🙂

[@epicstreamer]

You re in college but I m in school 🏫😂

[@jojoandnono7081]

Wow mam .. first time I got all the doubts about this topic cleared. And you will be surprised to know that I have completed my masters in biotechnology in 2009. What an irony and what a fortunate day today.

[@suhanasingh6714]

What are you doing like job ??

[@akshaybharti7361]

Watched your video for the first time, came back just to give feedback, the explanation was so good.

[@AbdulKadir-yy8sd]

ab k sabse beehatreen channel .... meko sare biotechnology technique and biochemistry ke question ka ans yhi se milta h .. thnx mam

[@Raka96876]

konsa exam h bhai ji

[@thedarklamp02]

The way you explained this topic with covering basics term explainations ... It's really helpful and worthy to watch.

[@BaaYo]

Thank you

[@swarnkaur775]

This is called perfect teaching

[@arshiarshi3756]

Ma'am u r the best teacher in techniques. 👍

[@mahimaShandilya1993]

Sanger sequencing at molecular level is now completely clear to me ... thanks a lot mam

[@bilquisara8582]

Wish you a very Happy Teachers’ Day, maam! You have always been the one I have looked up to!❤️❤️❤️❤️

[@anasff4358]

I m from pakistan and i really love your teaching method..... you gives us a bestest lecture

[@danialtyagi9749]

U r one of the best teacher ma'am , i just gone through ur videos during my last exam before nd did very well.. thank u sooo much

[@aloneprincess8080]

Really so nice

[@cricket_lover1717]

Which class you sturdy

[@ammarsayal]

bhai tu wese bi hmesha he jb paper sr pr a jayein tb he prhta hai

[@Ritisha132]

Mam ur explanation is incredibly awesome ❤ it starts from the basic to higher level which makes it more comprehensive

[@mazharmajeed5011]

Ma'am you are a great teacher, your videos are so helpful and easy to understand. Thanks to explain in easiest way

[@shamaiqbal3028]

You are one of my best teacher in the world...Thank you mam...from Pakistan

[@maharroase6847]

Teacher your teaching method is very good . I like your lecture and praper ourself for mdcat test. I easily understand your lecture. Keep it up.

[@shahbaloch4814]

Best method of teaching best teacher of youtube thanks a lot mam best understanding method

[@seemarjitkaur]

Ma'am you are a great teacher, your videos are so helpful and easy to understand. Thanks a lot ma'am

[@pranavpandey7347]

thankyou mam , you exaplain everything from very basic ( low to high) that helps alot in understanding better. 🙏🙏

[@sakshiupadhyay6043]

Waoooo your teaching way is florious thank you very much mam..❤❤❤❤❤

[@monikasingh-mr1rt]

Your explanation is so simple and easily understandable....Thanks alot ma'am

[@preetiyadav-cg7qp]

Your short and well explained viedo helped me a lott in my sem examination...... its really help me a lott nd also its save my time too becoz of ur short nd knowledgeable viedo.... thankzz a lottt..... 😊😊

[@Tariq1983]

Ur style of teaching is amazing

[@rajeshtayade907]

Thank you very much mam....you studies important consepts with very simple language.....thanks a lot

[@sombirzoology4115]

its amazing mam!! bhut acha and simple way me explain kya apne.....thanks you!!

[@pankajkumar5496]

The best KZbin chanal ❤️❤️❤️....I am big fan of you ma'am ji 😊🙏.... From RJ18

[@shanzaymalik864]

Mam I`m waiting for your next videos of DNA sequencing for last months....kindly update us why are you away from youtube channel???

[@GauravGhatak]

Please ma'am 🙏

[@rohith1772]

i think the reading the strand on gel was 5"--- > 3" , Because new strand will have the polarity of 5" -----> 3". Thank your for explanation.

[@meeradevi2934]

Thank u so much ma'am, your explanation is very well 🥰🥰 you achieve everything 😇

[@latikaanand5236]

Best explanation ever heard Thank you Ma'am

[@shaguftashazadi5349]

TEACHER G UR WAY OF TEACHING IS SO GOOD TNX FOR HELP

[@VipinKumar-br9kg]

Awesome teaching with excellent vexplanation 🙏🙏

[@abhishekrana5933]

bhot demag lgaya h scientist ne keep it up and you to beatifully delivered

[@zaidismm]

Watched ist time your videos ma'am Tysm uh explained very well! 🥰

[@divyakatiyar7101]

Amazing explanation!!!! All doubts cleared

[@pharmasolutionpakistan7668]

Mam you are working really hard for human being

[@tusharsingh9045]

Thank you ma'am you're awesome with explanations!

[@NituYadav4926]

Thnku mam...bhot hi ache se smj aya ye topic.😊

[@kutajdubey]

One of best explanation thank-you so much Mam I m following u from bayoo

[@ayeshasaleem5224]

Mujy bss ap ki hi smj ati hy love you from lahore

[@BaaYo]

Thank you

[@dikshhanemaa2826]

It's amazing ma'am bht h easy way me explain kiya apne...

[@omprakashsingh5228]

Way of explanation is tooooo good 👍👍👍

[@BaaYo]

Thanks a lot 😊

[@shobhitbishnoi007]

mam, ye to hum sequence tab bhi pta chal skti h, jab hum pehle primer add krte h, Template stand to vector ke through clone krte h

[@salmaakthersuma]

Mam Where are you, long time no video upload kiya apne. Are you okey?

[@betteru7676]

Really you have good teaching skills.......😁

[@samyakkanjilal1128]

You cleared my concept.❤️

[@mewo-fo5sz]

Because you are the best educator ❤️

[@bharatkumarprajapat8626]

wonderful viedo🥳🥳🥳 .I AM FROM IIT. BUT IT'S MUCH BETTER THAN PROFESSORS.

[@alaminafendy8679]

I am absolutely impressed.

[@BaaYo]

Thank you

[@takini4918]

This was very helpful. I am extremely grateful for your channel ma'am 🙏

[@ritasingh2027]

You are tha best teacher ❤️❤️🙏❤️🙏😊😊 mam

[@seggsumm.8955]

underrated channel

[@Ironman1234-D]

At 6.28 it is dNMP in DNA because in DNA while adding dNTP two phosphate come out to provide energy with the help of pyrophosphate ,this energy helps in adding dNMP in DNA

[@nehasangwan2000]

Your chromatography videos helped me lot in exams ma'm Thanku you so much.....❣️

[@umekalsoom9240]

AFTER DNA or RNA RUN on Agrose GEL ,what is the key point when some one show me pics of bands of DNA or RNA and said to me that which one is DNA band and which one is RNA????

[@omnivorousdiet4766]

A Very Happy Teachers Day Ma'am 🌸🌹🌹🌹

[@renukamaurya1966]

Mam......lacture is very helpful 👍🏻 Thank you mam🙏🙏

[@divyanshupadhyay3182]

Hello , there's a mistake primers are long oligonucleotide sequences

[@govindashahi4945]

You are the perfect teacher maam

[@siddiqui4u24]

If we want sequencing Genome of a human that is too big then how we ligate the genome with vector?

[@sumeshsudhakaran4823]

Mam.....In gel, they are short nucleotide chains right? Then how can it be interpret as a single nucleotide?

[@vijenderbudania2945]

Mam abb aap video kyu nhi upload krte?

[@kutajdubey]

Mam you explained it in a best way thank-you so much

[@Syedzadasuhail]

mam plz as there the only one each daughter strand Is formed each tube coz there will not form another daughter strand in each tube as the becoz DDNTP binds once hence no other strand forms after that then how you come with more than one strand in a respective tube

[@shabanaikram5278]

very clear and easy explanation.

[@MuhammadAli-ts6vg]

How we come to know the sequence of template strand before gel electrophoresis, like this os done to determine the sequence but from where we got the sequence GCATGCGA,,if we don't have this how can we match the sequence of every test tube with this..... please someone clear this to me

[@khushbubedi1426]

Too good And yes your's voice is like kangna ranaut😊

[@rolloflife2760]

Asslam o alikum mam MashAllah hamin ap sa bht sara teston information mil rhe or jaldi smjh a jati han. Mam aik request ha ka ap slide banana ka tarika be btayan.

[@draker.2523]

Ma'am you are amazing. Please come back

[@kanviaery2542]

How we will come to know which tube has G / C+T ? 🤔

[@Xak4753]

if we add all the dNTP's then how will get the exact length we only add one specific ddNTP and rest 3 dNTP's ... And in gel the sequence is 5'-3' not 3'-5'.

[@vedantroy92]

Thank you ma'am for this video. Can you please upload videos on genomics and bioinformatics

[@hardhittingfolk]

Why cant we start the treatment or management of particular disorder if childs reports are out.

[@saifaali2049]

This was very helpful. Thankyou makers

[@Dr.SRD19]

EXCELLENT explanation

[@abhijitpal7745]

Thank you ma'am... it's very helpful ♥️

[@adityaatripathy2463]

Ma'am u teach us in awesome way

[@sumandewangan8466]

Thank you mam this video is very helpful 👍🥰

[@SimaDe-us8nw]

How can you find the strands under the tubes ? From you can identify the strands under the ddNTP strands?

[@BaaYo]

No we don't find strand under the tubes.. we run this sample on agarose gel.. and according to strand length it got separated..

[@nehalji9158]

Thank you so much mam.. very nice explanation

[@fareedabatool7426]

Mam u explained very well ,it help me a lot ,thanku mam

[@cricketlover28258]

Very smooth teaching method i liked that

[@JYOtiRaNJanMANgaRaj]

Thank you so much 😊🙏

[@SandeepKumar-ns7fc]

Nice lecture mam very helpful

[@aseemkumar958]

Namaste MAM. i was watching ur vedios on microscope.i m interested in microscopic world.i m interested in microscope but confused about that which microscope should i purchase for home use which can fulfil all my necessity.(phase contrast,electrone,flurescence,.....)i want to see every micro object where living non living.plz can u suggest me which model can be right for me as there r several models.& which BP digital apparatus will be right for me.not high budget.

[@Dse629]

Thank you ma'am for all your video's

[@eyesnehasingh]

Thankyou ma'am you helped me with a doubt

[@jaydeepdumadiya2464]

Great ma'am ... God bless you...🙏🙏🙏🙏🙏🙏🙏🙏🙏

[@doraemon6853]

Ma'am plz provide me your telegram channel link... The link viz. Present in your description is not opening

[@N.a.q.1609]

Maam ye kaise pta chalega k which is our gene of interst

[@machoudhary1642]

Mam your description box i didn't found plz i want telegram channel link

[@adnanyounus6192]

Hi, m`am hope you are doing well. we are waiting for upcoming videos of sequencing.

[@sakshigirhe8492]

Well explained the primer part ...I don't found it in any other vedio

[@banjitapriyadarshini7295]

How to get the notes? I'm not getting the notes. Please tell me.

[@saurabhshukla7855]

Mam can't we sequence the complete human dna without fragmentation from any of the sequencing method

[@Al-Imtiaz]

Please also make video on PCR types with full explanation thank you ❤

[@BebargBaba]

The larger fragments should b ag bottom in gel agarose bands, n the shortest fragments at the top, but here the fragments depicted by u r reciprocal. But however u solved my confusion finally thank h so much

[@jyoti2446]

Ma'am ,main ek PhD scholar huin...aur mera work nematodes parasites ki dna sequencing ka hai....aap koi lab suggest kar sakti hai dna sequencing ke liye....aur kitna cost aata hai ek sequencing me.... please ma'am 🙏 reply jaroor kar dijiyega. thank you.

[@Trendycartoons-qy1lq]

Thanks a lot mam for clearing my all dought 🤩🤩