Lac Operon & Gene Regulation Made Easy - Best Explanation

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The lac operon (lactose operon) is an operon required for the transport and metabolism of lactose in Escherichia coli and many other enteric bacteria. Although glucose is the preferred carbon source for most bacteria, the lac operon allows for the effective digestion of lactose when glucose is not available through the activity of beta-galactosidase.
Gene regulation of the lac operon was the first genetic regulatory mechanism to be understood clearly, so it has become a foremost example of prokaryotic gene regulation. It is often discussed in introductory molecular and cellular biology classes for this reason. This lactose metabolism system was used by François Jacob and Jacques Monod to determine how a biological cell knows which enzyme to synthesize. Their work on the lac operon won them the Nobel Prize in Physiology in 1965.
E. coli encounters many different sugars in its environment. These sugars, such as lactose and glucose, require different enzymes for their metabolism. Three of the enzymes for lactose metabolism are grouped in the lac operon: lacZ, lacY, and lacA (Figure 12.2). LacZ encodes an enzyme called β-galactosidase, which digests lactose into its two constituent sugars: glucose and galactose. lacY is a permease that helps to transfer lactose into the cell. Finally, lacA is a trans-acetylase; the relevance of which in lactose metabolism is not entirely clear. Transcription of the lac operon normally occurs only when lactose is available for it to digest. Presumably, this avoids wasting energy in the synthesis of enzymes for which no substrate is present. A single mRNA transcript includes all three enzyme-coding sequences and is called polycistronic. A cistron is equivalent to a gene.
A second aspect of lac operon regulation is conferred by a trans-factor called cAMP binding protein (CAP, Figure 12.5). CAP is another example of an allosterically regulated trans-factor. Only when the CAP protein is bound to cAMP can another part of the protein bind to a specific cis-element within the lac promoter called the CAP binding sequence (CBS). CBS is located very close to the promoter (P). When CAP is bound to at CBS, RNA polymerase is better able to bind to the promoter and initiate transcription. Thus, the presence of cAMP ultimately leads to a further increase in lac operon transcription.

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Here are some notes too. kzbin.info/www/bejne/j4TZoJyaaNydmMWT_THE_LAC_OPERON

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Watching this, I realized the concept isn't even difficult. The textbooks and useless teachers make it hard. Thanks for making this understandable and logical! So happy I found your video.

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Frrr the textbooks almost made me memorize the stuff without really knowing what is what... now I feel like I've aced the topic😌

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Here are some notes too. kzbin.info/www/bejne/j4TZoJyaaNydmMWT_THE_LAC_OPERON

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The best explanation of this topic in KZbin. Thumbs up for the immense effort. The two mistakes I found was that 1. You once said DNA polymerase in place of RNA polymerase. 2.The I gene stands for inhibitor, and not, as you said, "inducer". The substrate of the enzyme beta-galactosidase, i.e.lactose or allolactose is the inducer.

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Extraordinarily Excellent Presentation and explanation on the topic of Lac Operon. The best ever detailed description of the concept with amazing animations. Well done. Expecting more videos on AP Biology topics.

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Really very nice way of teaching just like story telling. Appreciated 👍

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Very good explanation even if im not an english speaker, this video is so simply to understand lactose operon mecanism. Thank you from reunion island

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thank you! this was very clear and helpful. No part was confusing. Loved the relation of the letter B to promoter and polymerase .

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This is truly the BEST EXPLAINATION of Lac Operon . Good quality of audio and video and simple and easy.

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Good explanation sir.if possible can you post videos on gene regulation in both prokaryotes and eukaryotes

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12:30 The function of beta galactosidetransacetylase is that it activates the enzyme beta galactosidase.

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