Meselson and Stahl experiment

Meselson and Stahl experiment - This lecture explains about the famous meselson and stahl experiment that explains the mode of DNA replication. Meselson and stahl did the experiment to prove the semiconservative model of DNA replication. The experiment is performed by growing e. Coli bacteria in heavy nitrogen medium and then transfered to the light nitrogen medium to check the incorporation of new light nitrogen to know the model of semiconservative DNA replication.
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There's a mistake- after the first round of replication, conservative was ruled out, because conservative replication would not produce an intermediate strand... it would be light or intermediate, never heavy. The second round of testing was needed to distinguish between semiC and disp. models. Then it was discovered that only two gradients ever appeared; the medium and light. Dispersion would have multiple variants present based on its more random nature.

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Sorry. I must say it's the heavy form

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[@ushamogra5324]

u said 15N is a radioactive form of nitrogen but its clearly mentioned in ncert thats it is not a radioactive isotope

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N15 is not radioactive.

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It's heavy isotope.

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I understood the experiment from this Indian guy more than the explanation of Meselson himself. Good job buddy

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[@mr_panda9283]

Sir... I loved your explanation and the way you cleared each and every doubt of mine but in books , Google and chat gpt it is mentioned the the ¹⁵N used by meselson and Stahl was not radio active but a isotope of Nitrogen which is generally used in laboratory ... May be I am wrong but Thank you so much sir for this great explanation no one can explain like you to such complex topics Thank you sir🙏🏻

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It's heavy Nitrogen not radiolabaled

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