How a drop of pond water looks under the microscope 🔬 241

[@luigiolivobozzano5944]

Great video!!! At 4:17 the ciliate is a paramecium busaria, yes these are endosymbiotic algae inside it!

[@candynewton4945]

Glad I found your podcast, your commentary is educational enough to hold my interest yet not too scientific to lose me. Thank you for sharing your knowledge. It will be fun to follow from time to time. 🇺🇸🌹 from upstate New York 👍

[@Daniel-xu9ut]

Some of the most beautiful jewels can not be seen with the naked eye

[@DolanDolk]

I do enjoy this content

[@AussyCC]

Great work again @microbehunter. Gee u have a wonderful contrast bringing out the color nicely, is that a special contrast lens?.

[@Microbehunter]

Much easier! I did a color adjustment in the video editing program. But I also have a DIC microscope, which also helps.

[@AussyCC]

@@Microbehunter so cool thx 4 the reply mate. U do great work for us all. Could i unscrew say the 40x objective and screw in a rad DIC 40x one? Would be a nice upgrade.

[@Microbehunter]

This (unfortunately) does not work. DIC requires not only special objectives, but also prisms, filters etc. etc. and only high-end microscopes can be upgraded to do this. And it is expensive. However, there is a very attractive alternative, called Oblique Illumination, which also produces the 3D effect by creating shadows, similar to DIC. You might also want to experiment with Rheinberg and Darkfield. Video here: kzbin.info/www/bejne/hpzYZYduqJJjosk

[@AussyCC]

@@Microbehunter Thx for the nice answer mate. Yes i saw this vid and use the 3d filter sometimes. I likes your color mainly mate, great detail and quite impressive against others. I too will try n enhance my 14MP camera in the setting if i have them. I use my compound microscope for my medical cannabis growth/ dev, IPM, soil & runoff microbes. Your welcome to interview me for rad uses of the microscopes mate, i'm a post uni research med can student. Keep up your great work amigo!

[@rychei5393]

Do you think you can write the magnification and maybe and micro scale and filter type for each of your images in the corner?

[@adri9esp366]

Your vids are awesome! 😊😊😊

[@polytrichum1119]

Great video!Can you please make a video about dust mites?I tried to find them in my dust,but I failed.There are a lot of videos about dust mites,but no one mentions how to collect and find them.

[@deltalima6703]

Very nice! Thanks. :)

[@TuloMicroorganism-x1r]

Great video!!!

[@ronnykhalil]

i really cherish your videos. thank you!

[@____.__._.._]

Nice video. Is it made with phase contrast? And, does those crystals in diatoms (5:00) have a name?

[@Microbehunter]

DIC and a few clips in Phase

[@elyrose3480]

@@Microbehunter thanks for this insteresting video and comments, which model of microscope did you use for the DIC images ? i love how DIC images turn out, do you know which model is the cheapest or has the best ratio price/ quality ? thank for your help!

[@darryllim2969]

@@elyrose3480 If I’m not wrong he uses an Olympus BX53 with DIC

[@elyrose3480]

@@darryllim2969 thank you for your reply. I whish it was less expensive :(

[@nightstormoutdoors6080]

These are beautiful images and fascinating specimens. I wonder if you are using simple coloured filters between your transmitted light source and the specimens.

[@Microbehunter]

Color adjustment in the video editor made the background blue.

[@ihikefar]

Question- should you store a microscope with the nose piece positioned clicked on and objective or in-between objectives?

[@letszoomit365]

Thanks a lot! I hope no one is skip the start, its interesting too 👍😀 This spring I will try to make some video like this on too. I love to watch and want to try my microscope too 🙂

[@ashutoshtiwari8225]

Can you please put coconut water under the microscope from the tender coconut to possibly look for triploid endosperm nuclei?

[@Cevher7382]

Can you make a video about pickle juice?

[@Microbehunter]

Yes I can do that, but maybe it will not be very interesting... But I can try.

[@Cevher7382]

@@Microbehunter thanks

[@keithvoller4424]

I am very new to microscopy, so might be a silly question. How do you get colour on your slides, is it natural? I don't get that at all.

[@jwm6314]

I would be interested in a sample series from varying depths of established soil. A surface, 3", 6", and 12" sample and see how they vary. There is a lot of argument in the gardening world about tilling or not tilling and the theories about disturbing fungal and bacterial strata in topsoil. I personally don't give it much stock, I think whatever upset you cause is re-stratified in a month or so but that's just my hunch.

[@alisonburgess345]

There's ALWAYS pond water lol! very interesting indeed.

[@Haven_city_civilian]

This is a water planet my freind.

[@nightstormoutdoors6080]

One more question, Oliver. You said that your camera also captures a very large field of view, therefore there is very little empty magnification. What do you mean by empty magnification? Surely, the field of view can only be as large as the field of view through your microscope objective and eyepiece lenses. I should add that I know about sensor sizes and how they work. I know much more than I need to about still photography, but not so much about videography.

[@Microbehunter]

When the camera only takes an image from the center of the field of view (and not the whole), then this image is magnified "artificially". It will appear blurry. It is magnification without additional information (beyond the resolution of the microscope) and this is then called "empty magnification". So this is magnification that does not add "value". You can also get empty magnification by using very strong eyepieces. It will appear larger but more blurry. In case of mobile phone cameras, they catch the full field of view, but the image will appear in a circle and you lose pixels this way. You have to crop out a rectangle inside the circle and therefore you again lose some image information.

[@nightstormoutdoors6080]

@@Microbehunter: I have seen this in practice. Thank you for the reply. I do like to keep the circle or at least enough of it to see the diameter of the field of view as seen through the microscope lenses. I almost always use my 10x eyepieces. Putting that circle up on a laptop screen allows me to roughly calculate the size of the specimen by measuring it in mm in relation to the diameter of the field of view, then converting those sizes to microns. If I crop to a rectangle, like you said, I lose more pixels and also any chance of getting measurements of microbes or blood cells accurate. Measuring sizes of specimen is one of my priorities and indeed pleasures. If I keep the circle, it doesn't matter how many times it is zoomed or magnified, the scale of everything within the circle is constant. On video, I usually zoom in to around 2.5 times on my phone camera, this leaves the edges of the circle visible enough to take a measurement across the diameter on the computer screen. So far, I have been very happy with my still images but always looking for ways to make improvements, as there is always room for improvement. I do have a video software program and it can adjust contrast and sharpness, but it is not anywhere near as complex as your software is. My phone camera actually has 13mps not 5 as I stated in my previous comment.

[@MadHatter11371]

What microscope did you use for this ?

[@biologyenthusiastic4963]

Fan of you sir

[@nightstormoutdoors6080]

I am a bit confused about your photography techniques. I remember in an earlier video of yours, you said that 5 mps can be even more than enough for microscopy, yet you use a Canon DSLR with far more mps than 5 yet your videos are absolutely crystal clear and pin sharp. How can this be? How can I produce images like that with a 5mp phone camera?

[@Microbehunter]

Full HD video (1080p) has about 2 megapixel. I use 4K, which has about 8 Megapixels. This is more than the microscope can deliver and the 4K resolution is much higher than what a normal monitor is able to display anyway (and youtube videos are shown small). So it is not a resolution issue alone. the reason why the images appear so clear is because of several reasons: First I have a DIC microscope, which makes the specimen appear to cast a shadow, and increases contrast. So the edges are more visible and you get the subjective impression that the image is clear. Second, I do a lot of editing of the original footage. I use the free video editor daVinci Resolve (can be downloaded), which allows me to adjust pretty much anything. The software is powerful but complex, however. The camera also captures a very large field of view, so there is very little "empty" magnification. So there are several factors that play a role. You will be surprised what simple contrast enhancement is able to do to improve the image quality. While no image processing technique is able to do miracles, I would first do a contrast enhancement (levels adjustment).

[@alexandredespres9294]

Hello. Good video btw. How did you get that bleuish background?

[@Microbehunter]

I changed the color in the video editor (DaVinci Resolve). Original color is gray.

[@JonFrumTheFirst]

Have you ever noticed that when people talk about life on planets orbiting other stars in the galaxy, they never mean this?

[@mipacem]

whoa

[@Crumbed304]

I have an question again Can you find Paramecia in bananas peels? I havent found any. I found allot of bacteria by hunting for Waterbears And some ar cilliates begause they kinda big for an typical bacteria. Great video 😃

[@Microbehunter]

Paramecia are found in ponds and streams. You can use a small banana peel to feed them, but they do not live on bananas.

[@Crumbed304]

@@Microbehunter Ok

[@davewinch7677]

Someone asked me, "What does tap water look like under the microscope?" Well, if you are drinking it, you better not find anything. 😲 Pond scum is the best when it comes to exploring with a microscope.

[@Ide2gue]

3:11 I thought it was bacteria. Turns out it's not

[@anna303colorado]

We collected water and saw nothing under our microscope. What kind should we get to see organisms?

[@Microbehunter]

put some solid stuff from the bottom of the pond etc. on the slide, not only water.

[@anna303colorado]

@@Microbehunter thank you for your quick response we just found your channel and are looking forward to learning more

[@SuperBoppy]

That water is not disgusting if you're a microbe. 😃

[@breatharian2009]

Please inspect mineral water.

[@johndubose6589]

I lose your audio in this KZbin clip.

[@oliver1834]

hello

[@lotharmayring6063]

it is not Calzium it is Calziumcarbonat......basic chemisty