Microbiology - Bile Esculin & 6.5% NaCl

[@JA-eq5um]

Of course I could do that. But I would not want to deny you of a very opportunistic and positive teaching moment.

[@patricktracy9947]

Your question is pretty non-descript. What is the difference between the two? One is an agar and one is a broth. One is used for identifying gram-positive cocci and one is for isolating and determining the lactose-fermenting capabilities gram-negative rods.

[@spidermonkey4329]

I am not taking your class but your video was very helpful. Thank you very much.

[@patricktracy9947]

Hi SM...thanks. Good luck in your studies.

[@alilueve2766]

How do we produce 6.5% Nacl for enterococcus please?

[@veenagautam618]

please can u tell me how to make bile tolerant bacteria results on paper.....?? means how to generate results actually... which type of format we should use...?????

[@patricktracy9947]

Hi Veena, We don't do bile tolerance on paper.

[@AbdulAzeez-hy5jm]

Sir How to check bile salt tolerance of bacillus coagulans in food Which online platform in India provide the specific bile salt Hoping for quick response

[@patricktracy9947]

Abdul Azeez, I have never tested B. coagulans for bile salt tolerance, so I can't help you. Sorry

[@nruwaili5494]

Should we do zigzag on bile esculin tube ?

[@patricktracy9947]

It doesn't matter if it is a straight line or zig zag.

[@zibanhgi8472]

You are a life saver

[@patricktracy9947]

Well thank you.

[@BaconNDCheese]

what happens if half the slant is black? is that still a positive

[@patricktracy9947]

Yes

[@JA-eq5um]

what's the difference between 6.5% and MacConkey agar?

[@patricktracy9947]

Good question. Just as I would tell my students when I don't know the answer to a question that can be easily investigated: Look it up.

[@mysslesa]

MacConkey agar is a solid medium that is selective for Gram negative bacteria and differential for Lactose fermenting vs non-lactose fermenting, there is no salt. The 6.5% is NaCl and the goal is to see which organism can grow in the presence of salt and which one cannot, so you only look for turbidity. I am sure you have already found the answer but in case anyone else was wondering...

[@mariamalqhtani1858]

Seems very easy I don’t know why I made mistakes in that 🌚💔