mRNA Processing

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Capping, polyadenylation and intron removal are the steps of RNA processing
Since Transcription and translation occur concurrently in bacterial cells; when the 3′ end of an mRNA is undergoing transcription, ribosomes bind near the 5′ end to the Shine-Dalgarno sequence and begin translation.
Since transcription and translation are coupled, before protein synthesis, bacterial mRNA has little opportunity to be changed.
However, in eukaryotic cells, transcription and translation occur in the nucleus and cytoplasm respectively
In the nucleus, when transcription is completed, mRNA moves to cytoplasm for getting translated in to protein
after transcription, eukaryotic mRNA is altered extensively.
Changes are made to the RNA molecule’s 5′ end, the 3′ end, and protein coding portion.

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