NGS Course, Session 2: RNA-Seq. Analysis

[@VanduTamraparni]

Sir one of the best video so far i have seen very detailed explanation of rna seq work flow and how to use galaxy

[@SK-fn5fc]

This is beautifully explained. Please make more videos on related topics!

[@EmilyToscano]

Very useful and interesting tutorial, Thanks a lot!

[@hanumanaik2396]

great explaination Thank you

[@juanpablovalenzuela8924]

Thanks!! I'm trying to understand Galaxy, your videos (3) are really helpful! (Y)

[@musicbf]

What do one do if you do not have a transcriptome file, a gtf.gz-file?

[@sutrishakundu2147]

I have a transcriptome file which I want to do this analysis but I don't have a reference genome...so I have to de novo assembly...can you please guide how to do that?

[@gargibindal271]

Hello, I am trying to do analysis on RNA seq data obtained on E. coli. But when I run String Tie or htseq count, I am getting errors such as An error occurred with this dataset:format tabular database ? or An error occurred with this dataset: format gtf database ? I am using gtf file downloaded form NCBi, I am unable to resolve this. please suggest what can be done in order to complete the analysis