Part 16: Column Chromatography - Frontal, Displacement and Elution Techniques

[@arunshelkeavs2918]

Tomorrow is my exam...i was totally blank about the development techniques of chromatography..but u rock sr....Thank u sr...with comparison u explained it very well....

[@pkclasses100]

Please share the video links with your friends and help us to spread the knowledge.

[@aksaisac]

Great explaination...easy to understand... thankyou so much sir😊

[@dierdrekunene3165]

❤❤❤❤❤. Can't thank you enough sir. This was extremely helpful 😊

[@prasannakumar1614]

Great explanation sir.. i improve my knowledge from ur channel... Thank you very much puspendra sir 😍

[@pkclasses100]

Thank you for the appreciation. Keep Watching and Share the Video Link with your friends and help us to spread the knowledge. 👍

[@nagirikatakamsateesh6121]

Thank you for your explination and your explination is very welll

[@yathiroyal2002]

Excellent explanation sir thank you so much

[@pharmd221]

Fractions of the samples come out of the solvent Elution analysis Simple elusion Gradient elusion

[@subhajitpurkayastha2260]

Thanks sir it helped a lot to understand

[@himanshukumar2110]

Thank You sir for such a good explanation. Thank You Very much.

[@user-mohamedibrahim2]

I'm from sudan thank u alot sir for this vedio♥️

[@pkclasses100]

Welcome!!!!!!! Keep Watching and Share the Video Link with your friends and help us to spread the knowledge.

[@pharmd221]

Gradient elusion Composition of the solvent differ

[@pharmd221]

In displacement analysis used to separate compounds having greatest affinity for stationary phase .

[@user-vl8ns6ro7b]

It really helped me alot thanks dr ❤

[@pkclasses100]

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[@user-vl8ns6ro7b]

Dr. Puspendra Classes i will now am gonna share it with my friends 😊

[@pkclasses100]

Thank you

[@pharmd221]

Column chromatography Elution analysis All compounds in 100 percent pure form is obtained Mixture of sample is administered a b c The band

[@poomapooma9906]

Thank you for your clear explanation sir👏

[@prernabansal8995]

Thanks!!! Very well explained!!!

[@ZafarIqbal-pf7vs]

Sir great effort ...:-):-):-)help a lot I also share with my friends

[@pkclasses100]

Thank you for the appreciation.. 👍🏻 Please share 👍🏻

[@seshagiriaitha1184]

Osmmm sir........ very helpfull lecture.....,😃

[@pkclasses100]

Seshagiri Aitha Thank you

[@upasanaraturi7384]

Sir this video is very helpful for us🙏

[@abhipsasatpathy179]

It was really very helpful.Thanks Sir

[@pharmd221]

In frontal analysis compound Wih least affinity for stationary phase and higher affinity for mobile phase eluted out . Only one compound in pure form is eluted in frontal analysis.

[@shreemclasseskota2267]

Is used sample always solid to put in a band form ......

[@shivkumarswamy861]

Sir it's good..bt u didn't gave reason in ellution analysis that y that solvent concentrating after each sample???

[@ammukutty2202]

Great class❤️❤️❤️

[@arshisheik9980]

Well explained👏👏👏👏😊😊

[@pkclasses100]

Thanks!!!!!!! Keep Watching and Share the Video Link with your friends and help us to spread the knowledge.

[@DeepanjaliSrivastav]

Thanku sir 🙏🙏🙏❤❤

[@pharmd221]

Simple elusion Stepwise elusion Gradient elusion

[@madhutaylor8302]

Sir one doubt..if they ask elution methods..shud we include all the four methods..i.e isocratic, gradient, frontal, displacement

[@hidakhan07860]

Thanks 😊

[@aqsaaqsarana7500]

Good job

[@shaliazahoor9905]

Sir plz present about low pressure column chromatography in which columns,matrix material,and stationary phases

[@pkclasses100]

Already explained please see the description 1st link. Contains all videos.

[@pharmd221]

Quantitative analysis Solvents evaporated Pure form sample obtained Components having greater affinity for stationary phase come out

[@MJTechs]

sir elution techniques and separation techniques both are same right in column chromatography

[@pkclasses100]

All techniques are used for separation.

[@hidakhan07860]

Thanks sir😊

[@shreemclasseskota2267]

Eg. Of displacement agents..?

[@pharmd221]

Isocratic elusion means simple elusion

[@sejalmalik3284]

Why is A in every fraction in frontal analysis? Some one please explain

[@pkclasses100]

It's an example only. It may be B or C also

[@sejalmalik3284]

@@pkclasses100 I meant one compound will remain throughout the stationary phase or its just shown that A is running through every fraction and finally reaches end

[@kashafroy3990]

Sir can u please differentiate btw frontal analysis and isocratic elution method? I mean in displacement method we use displacers and in gradient elution we change composition of mobile phase so these are clearly different methods but what about frontal and isocratic how can a person tell a difference in these methods without seeing the results?

[@kavyaviswanath9386]

thank u so much sir

[@kaleemsakhani5807]

why we don't get pure result in frontal but get pure result in eluation but the process of both is same plz explain sir

[@pkclasses100]

That depends upon the development of solvent system. If analyst is not able to develop solvent system for elution technique then only analyst will do frontal analysis because of no better technique available else elution technique will be done.

[@mohammedzeeshan8832]

In frontal analysis no separate mobile phase is used insted wo keep on adding sample solution(that act as a mobile phase)thats why we do not get cmplt separation where as in elution process we use a mobile phase which do not contain any sample solution.

[@pkclasses100]

Right 👍

[@umairhabib4618]

sir how graph is different in elution technique if all compounds give 100% result.

[@aiswaryanath9844]

Mixture may have varied concentrations of components .

[@aiswaryanath9844]

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[@haridev3815]

Thankyou

[@vikeshtripathi6755]

Sir I need your help on retention mechanism in column chromatography

[@ritikachouhan3529]

Thank uhh sir

[@prasanthkandelli4537]

Explain Chromatography types sir please explain

[@Lalitha.sj2019]

Pls do more videos for us

[@pkclasses100]

I'm trying to upload maximum number of videos.

[@Lalitha.sj2019]

Nice tnq

[@lemmemgammi630]

Thank you sir

[@ajayghosh1338]

thank u sir

[@pkclasses100]

Welcome. Keep Watching and Share the Video Link with your friends and help us to spread the knowledge. 👍

[@nagaratnamalaakula5513]

Tq sir

[@nikitapayamalle8048]

Nice sir

[@pkclasses100]

Thank You. Keep Watching and Share the Video Link with your friends and help us to spread the knowledge.

[@shaliazahoor9905]

Sir matrix materials k baray ma smjain

[@pkclasses100]

Your question is not clear to me. Please elaborate.

[@pharmd221]

In displacement analysis 100percent pure compound not obtained

[@berryelmasry1442]

مفيش حد يشرح كلام ده بالعربي 😢

[@achalakp705]

Thank you so much sir

[@palaparthisandhya1653]

thank you sir