PCR types

[@x4rdas]

2:56- COLONY PCR 7:47- HOTSTART PCR 12:57- NESTED PCR 19:14- MULTIPLEX PCR 26:36- INVERSE PCR 32:25- LONG ACCURATE PCR 35:25- REVERSE TRANSCRIPTASE PCR 40:58- REAL TIME PCR

[@ahmetdemirli1]

3:04 Colony PCR 19:18 Multiplex PCR 26:36 Inverse PCR 32:16 Long Accurate PCR 35:25 Reverse Transcriptase PCR 40:58 Real Time PCR

[@kristianadrian7335]

+ahmet demirliçakmak Thank you

[@somosreeghosh8662]

Thanku

[@XD-pe3ur]

I love you

[@shameem_07]

Thank you

[@LIGHTSBIN]

Our saviour as always🙏😇

[@shomusbiologyofficial]

Thank you so much for appreciating my efforts

[@md.shahidalam6358]

love to gain important concepts by your easy way of explanation..... thankx

[@HashiniChandrasiri]

Thank you very much your lectures helped me alot with my biochemistry exams..

[@komalijaz9960]

nice lectures.......your lectures help me a lot to understand different topics

[@shomusbiologyofficial]

Thank you. Glad u liked my lectures.

[@komalijaz9960]

+Shomu's Biology sir when a gene is cloned ...in ahost cell the gene first synthesize RNA or not to express itself ?

[@kamalkamal-ul6ly]

Shomu's Biology what is this program you are using ?

[@manojbongale1994]

Super sir,thank you very much please make more videos of Biochemistry,biotech, microbiology,Bioinformatics.

[@shomusbiologyofficial]

You're welcome. Glad to hear that you're getting benefit from my lectures

[@indranibanerjee4296]

I got a bit confused with hot start PCR coz I didn't understand the reason why we would use it ? kindly explain that topic a bit clearly and thank you for explaining the whole of PCR.

[@juhisharmaprasad7381]

amazingg video sumanji........thanx a lot in making such simple presentation

[@Alexx2696]

Thank you for this super helpful video. Can you make a video explaining TAIL PCR (Thermal asymmetric interlaced PCR)?

[@roungson09]

Thanks a lot. This is very useful for me.

[@satokiumi5708]

Thank you very much.. It is very understandable and informative

[@parbatipokharel1091]

is it so. I am not sure

[@TheMagodana]

thank you so much for the lecture, particularly in inverse PCR, however, since you dont know the sequences the you want to amplify, linearising the circularised DNA may result in a cut somewhere in the unkown sequence and prc wont work,

[@dorisvm9801]

Very helpful thank you for the video!

[@shomusbiologyofficial]

You're welcome

[@Nassomah]

you are awesome.. wait for more details on real time PCR.

[@tasrash1529]

Thank you so much, it's very useful! Great job!

[@asmaelaouina7047]

its great i've understood many details, can you please make a vido of ASO-PCR principe ? i have some questions on that technique...

[@juhichauhan6569]

asmae laouina you are from

[@mhamado.abdullah6925]

Well done, thank you❤

[@shomusbiologyofficial]

You're welcome. Glad to hear that you're getting benefit from my lectures

[@rajshekarmandla3905]

Nice and clear explanation.Thanq sir

[@thepowerofthehardwork2142]

Thank you so much sir

[@shomusbiologyofficial]

You're welcome

[@sushantsharma1926]

Doesn't the length of amplified product or the length of the amplified strand reduce in subsequent cycle? How do we overcome that?

[@karenhenry1014]

Thank you

[@shomusbiologyofficial]

You're welcome

[@ruthpallepogu9456]

Good evening sir Naku icmr exam udhi and PCR gurichi bagha cheparu

[@bhargavireddy5730]

Sir can u plz explain abt ERIC PCR

[@sjchun79]

Nested PCR 12:57

[@tubanafees5327]

How will primer anneal at low temperature in hotstar pcr when the template strand is double stranded?

[@djalitanaful]

thank you, it was a very helpful video.

[@jyotigoswami3764]

Thanks so very much sir!!!

[@shomusbiologyofficial]

You're welcome

[@acidceed]

how is the rna digested? i use iscript mix which means that all the enzymes that will be used will be in the same tube. is this a heat specific thing again? where the rnase works at a particular temp but doenst affect DNA? thank you for the videos. you have been very helpful in my studies :)

[@Oscar-vy5br]

Thanks, very informative.

[@kedarprasadpyakurel9528]

Pls throw light on branched DNA assay

[@sarikagupta7586]

How about RACE (Rapid amplification of cDNA ends) PCR?

[@kameswararaoneelapu2938]

thanks Sir. nice video

[@sjchun79]

Hot start PCR 07:49

[@vandanasingh5022]

sir is it mncl2 or mgcl2 ? because we are using mncl2 in RT PCR

[@harikeshyadav1011]

Sir nested PCR best hai ya rt pcr

[@سبحاناللهوبحمده-ب2د]

Great

[@shomusbiologyofficial]

You're welcome

[@kanchanyadav000]

bacteria have no nucleus, it is a prokaryotes organism, but you told in this lecture basically in the colony PCR nuclear extract comes out, is it true

[@shomusbiologyofficial]

I meant genetic material of bacteria comes out

[@ranajaysaha7274]

Thanks a lot

[@shimaahisham575]

Where is the Gap PCR ?

[@kamalkamal-ul6ly]

what is this programme you are using ?

[@bellabihah419]

There's no allele specific PCR

[@reddymahesh2244]

ARMS PCR???

[@monatalaat317]

PLEASE CAN U DOWNLOAD IT POWER POINT

[@MOHAMMEDALI-vq4xi]

Can i get the ppt?

[@shomusbiologyofficial]

I didn't have it

[@relajamal8871]

thanx L:3

[@toobaaleem673]

in situ PCR ....???

[@tomatogogo100]

Good job ..BUT.. you're pronouncing of english language is so bad ... I don't understand most of the words

[@alimoucamara7046]

thanks a lot