qPCR details | quantitative real time PCR | RT PCR | Biotechniques

This video talks about qPCR in details | quantitative real time PCR | RT PCR | Biotechniques.
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[@ghodbaneamira8228]

best explanation ever, thank you.

[@animatedbiologywitharpan]

Please share with friends

[@quickbiology5159]

Superb Arpan i have joined PhD lab and your videos are helping me when i was teaching, they are still helping me in research ,you are a person to whom i would definitely would like to meet you one day..

[@animatedbiologywitharpan]

Thanks a lot. I am really happy to help

[@cutieaadi9599]

Please don't forget to mention in acknowledgement!!!

[@ominibioentrance6458]

Good explanations........what if the ct value is same for the control and treated sample. Say ct vlue 26 for both. Does that mean in gene expression or the transcript level is smae in both control and treated sampke

[@joshuastavos4376]

Can you explain the disparate CT values for the 18s RNA control for the Control 1-3 groups and the Mut1-3 groups? Is it because there is more 18s in the Mut1-3 groups ( possibly due the different intracellular environment in the Mut 1-3 groups??). I would have thought that the 18s RNA level would ve comparable in both groups at the outset.

[@fatmabetul5985]

Sir, You are amazing at explaining the lab techniques' protocols and principles. Thank you for your help and the effort you pay for us! My best regards from Turkey!

[@animatedbiologywitharpan]

Could you please help me by sharing my contents with your friends group/ college group. I put huge efforts in making these videos but unfortunately not a lot of people are watching this.

[@fatmabetul5985]

@@animatedbiologywitharpan it is my pleasure. I will do my best to help you!

[@ShambhaviTrivedi-ub2yi]

can you please shoot a video on how to plot graphs/ curves of different datasets using graphpad prism.. Really need to learn this

[@animatedbiologywitharpan]

there is a course on this in my website. Launch date 5th April. It's only 250rs. email us at [e-mail] such that we can send you link.

[@ritabrataroy9797]

Your videos are just great...can I ask you a sort of personal question if you don't mind? How much time are you expecting to get your doctorate degree,..as I am entering in the research world,I want to know because most of the institutes take more than 6 years generally, previously I thought that 5 years is enough.

[@animatedbiologywitharpan]

I got MSc + PhD in 6 years

[@mithilasandeepa8999]

😅😅😅😅😊😊😅😅

[@sarahjustme]

At my institute a PhD takes 4 years and often extends to 4.5 years, but not longer.

[@Rvish_mini.vlogs7]

Most adequate videos of experiments

[@animatedbiologywitharpan]

Could you please help me by sharing my contents with your friends group/ college group. I put huge efforts in making these videos but unfortunately not a lot of people are watching this.

[@Rvish_mini.vlogs7]

@@animatedbiologywitharpan yes of course

[@Rvish_mini.vlogs7]

@@animatedbiologywitharpan i am also from medical research

[@enriquecorona3184]

Explantions are good to understand, however would suggest that speaking slower the understanding of your saying would be better.. thanks for posting the video

[@animatedbiologywitharpan]

Thanks for your suggestion.Could you please help me by sharing my contents with your friends group/ college group. I put huge efforts in making these videos but unfortunately not a lot of people are watching this.

[@Universe12355]

Can you please do a video interpreting further CT value and melting curves and how to interpret them? By saying for example this CT value with this Tm indicates primer dimer …. Or For example, what would a Ct value of 39 and a Tm of 64 mean when found in a negative control ?

[@animatedbiologywitharpan]

I will try

[@Universe12355]

@@animatedbiologywitharpan thank you so much!

[@rb11ism]

Great video...but Sybr green technique is now obsolete

[@animatedbiologywitharpan]

No no it's still used. The taqman probe based chemistry is bit expensive

[@niraxlevi9930]

Absolutely amazing ❤wow i watched many videos and i didn't understand it you saved my *ss 😂 also i have a question, the DNA fragments that are undetected aren't measured? Let's say for the sake of simplicity, they are 50 undetected ,their number isn't added ?

[@animatedbiologywitharpan]

very unlikely the amplified DNA molecules would not be detected because the sybr green dye binds to dsDNA pretty strongly

[@alhassanmohammedawal3851]

can you explain to me mathematically, how the CT values decrease with increase concentration.

[@animatedbiologywitharpan]

The Cycle Threshold (Ct) value in quantitative polymerase chain reaction (qPCR) is inversely related to the initial amount of target DNA in the sample. A lower Ct value indicates a higher concentration of the target DNA in the starting material. In qPCR, the amplification of the target DNA is monitored in real-time, and the Ct value is the cycle number at which the fluorescence signal crosses a predefined threshold. The fewer cycles needed to reach this threshold, the higher the initial concentration of the target DNA. So, a high DNA concentration in your sample would generally result in a lower Ct value, and vice versa. Researchers often use Ct values to estimate and compare the relative abundance of target DNA across different samples in qPCR experiments.

[@alhassanmohammedawal3851]

thank you Do you have experience with Quant studio5? @@animatedbiologywitharpan

[@user-ty3lo2if9q]

Do you know how to calculate the percentage of GMO in food using qPCR please?

[@animatedbiologywitharpan]

I Don't think it's possible

[@TheAmrein]

why is the 18s the one that varies so much? isnt it supposed to remain constant?

[@animatedbiologywitharpan]

This was a hypothetical data

[@joshuastavos4376]

@@animatedbiologywitharpan you kind of answered my question which I just added, but I ask almost the same question for confirmation.

[@yasminemaanani8215]

Merci !

[@DevonPhoenix]

Not diagnostic

[@animatedbiologywitharpan]

Why do you think so ?

[@DevonPhoenix]

@@animatedbiologywitharpan the person that invented it carey mullis stated that. But maybe im wrong i dont know.

[@drsarathkrishnanmp8449]

Diagnostic role is also there

[@sandeepb9397]

Full Benglish !

[@animatedbiologywitharpan]

Unlike others i flaunt my Benglish accent. If people love the french mixed english then whats the problem with Bengali.

[@aherodoingcriminalactivity]

​@@animatedbiologywitharpan Your Benglish very easy to understand and comprehensible for me. Thank you brother 😊

[@soudas08]

@@animatedbiologywitharpan proud of you…

[@milenlozanski456]

Major tool of my BsC and MsC thesis! 🧬🧪🧫

[@animatedbiologywitharpan]

Glad to hear that. I hope this video was good in explaining