Phage Display

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Charlie Abbott

Күн бұрын

Used for the Cambridge Science Festival to explain our Phage Display Technology

Пікірлер: 29

@carl-johanhorberg1399 6 жыл бұрын

Thanks! Finally an explanation that puts it into a functional context.

@veraltol170 6 жыл бұрын

i have been in difficultes somme days to cleary understand this method. i was stressed but this video helped me a lot! Merci beaucoup aux auteurs;

@Biomeducated 5 жыл бұрын

The fishing can be done in several ways, depending on the target of interest and how it can be recombinantly produced (or not). For instance, you can interrogate your library of phages for selection on solid phase (where the antigen is coated on a plate), or in solution (where the antigen is coupled to beads). If you're antigen is difficult to produce (for instance a GPCR), you could select in solution on recombinant CHO or HEK cells expressing the target (allowing proper folding of the target). Furthermore, selection strategies can be designed for diversity, or for affinity, all depending on the goal of the discovery strategy. Informative introductory video on Phage Display! Cheers

@yandongan1345 4 жыл бұрын

so useful，thank you

@danielgladish2502 3 жыл бұрын

Wow! Great explanation and super interesting technique! Very high throughput

@chrissun9960 10 жыл бұрын

Very clear description. But I am confused how people know the gene can be expressed as an antibody inside the phage.

@scottcampbell6390 6 жыл бұрын

The antibody is displayed on the phage coat, which is outside of the phage capsule. The phage structure is built by the host bacteria. If we insert the gene encoding the desired antibody inside the coding region for the phage coat, the antibody will be expressed on the coat when the host bacteria replicates the phage.

@pluvia13 5 жыл бұрын

@@scottcampbell6390 yeah so because we cannot rely on the bacterias ability to correctly fold an eukaryotic molecule one has to consider that this molecule could differ in structure from what would be achieved in an eukaryotic cell using the same gene right?

@daoyuzhang1648 4 жыл бұрын

This could be what was used to generate the 100x higher-binding-affinity ACE2 RBD in Covid-19. Such high binding affinity have never been found in nature for betacoronaviruses in general.

@belindashaw1847 6 жыл бұрын

Would this be useful to other protein expression platforms, say in plants such as tobacco? And if you wanted to make a unique antibody that you didn't have the antigen for how could you go about that...? Thanks for the video was very helpful :)

@amonoracheal9118 2 жыл бұрын

Thank you for making it clear for me:)

@Biomeducated 5 жыл бұрын

Excellent explanation and animation! It's also used to 'fish' for Nanobodies! If you don't know what Nanobodies are, I made an introductory video on my channel. Great video, Charlie Abbott!

@lauraricarte7938 6 жыл бұрын

this is simply fantastic

@pavarinodu 4 жыл бұрын

Wow! That's fascinating!

@oidualclaudi0 2 жыл бұрын

Is this an alternative for the use of animals like rabbits and mice to produce antibodies?

@paffnblunt500 6 жыл бұрын

from where do we take the genes at the beginning?

@Biomeducated 5 жыл бұрын

Well, you could trigger an immune response in mice by injecting a human target, then you isolate the B-cells from the mouse, and then you extract the RNA from those B-cells, and you got yourself a library :)