I appreciate the amount of effort you put into your lectures and illustrations.
@Sam-sw7sw Жыл бұрын
Oh my god thank you so much! I finally understand the entire process because of this video!
@alaamufawwez16029 жыл бұрын
Ur videos help me in my genomics exam.. Thanks aloooooot ⭐️⭐️⭐️⭐️⭐️
@randomassguy3 жыл бұрын
Fantastic video this greatly helped me understand this topic
@adimchinakaonyejekwulum32934 жыл бұрын
Thank you. Your videos are super helpful.
@alishbamirza47094 жыл бұрын
THANKS A BUNCH !
@XoXHannah41XoX8 жыл бұрын
You said that you cleave the larger DNA fragments into smaller gene fragments... but if the functions are not known, how do you known when you're cutting a gene itself out and not halfway though a gene? Do you look for start/ stop codons first or something? And how do you separate the genes into different beakers, is it based on differential centrifugation...? I'm a little confused!
@mayamaya-ry3eg8 жыл бұрын
yes im confused as well. im assuming you can take the dna that has been run through the gel and then clone it and put them in beaker? i might be wrong, ive never run the gel electrophoresis before
@barrylitser37936 жыл бұрын
you have to carry out partial dna digestion to improve your chances of having atleast one intact copy of each gene because indeed restriction enzymes don't respect gene boundaries. Later on you can look if the gene of interest is present by using a complementary strand.
@ornitcohengindi903 жыл бұрын
you are so clear! i love your videos please make more
@myriampinkas74784 жыл бұрын
Does the restriction enzyme cut precisely one gene at a time or does the fragment sometimes include more (or less) than one gene? How does the restriction recognize where to cut?
@gail41007 жыл бұрын
Thank you so much :) this was very helpful
@nazninkhan95954 жыл бұрын
thank you sir......it's very heipful for me...…..thanks again
@zeinabebrahim76847 жыл бұрын
your video has helped me alot🌟🌟🌟🌟🌟
@lbarbaric116 жыл бұрын
Explained so well!
@soheebwains15076 жыл бұрын
this was awesome, thank you!!!
@andredvs6 жыл бұрын
your videos are awesome. thank you.
@hoangphucnguyenle765 жыл бұрын
many thanks! I fink it very useful and easy to comprehend
@glilyjebamalardaviitkharag87635 жыл бұрын
Isn't it an audacious claim to make when you say that the restriction sites are present exactly at the intersection of 2 genes? I believe the restriction site locations will be extremely impossible to predict, as we never know where a palindromic sequence is supposed to be.
@fishyfishfishing_3 жыл бұрын
As far as I know, scientists use many different restriction enzymes on different copies of one genome to be sure to have the maximum representation (and to include the sequence they're seeking)
@kotikotordia74969 жыл бұрын
You're the coolest *_* thanks a bunch
@rezayekta21533 жыл бұрын
Thank you very much
@Mr007amir0072 ай бұрын
wow u r very good!
@ArslanFathullah5 жыл бұрын
Do we still (now) need a phage vector to replicate the gene fragments? Why can't we do a PCR to amplify the gene fragments in large number followed by insertion into e.coli and screen for our gene of interest?
@SharpSapphire Жыл бұрын
Where is the “plasmid” version specifically please
@mahdiyehbigham63572 жыл бұрын
great!
@jyothisajjanapu20078 жыл бұрын
can you tell me how the probe is going to get hybridised with the dna of interest?and how we are going to seperate that hybridised dna for further process?
@rajwant_kaur4 жыл бұрын
How many recombinants has to be screened with 90 percent probbality of getting a gene if the size of genome is 8000kb and library was constructed in puc vector Please answer
@sabaali70483 жыл бұрын
0.15
@sabaali70483 жыл бұрын
Use Clark and Carbon formula N=ln(1-95%)/ln(1-1/3)