The use of Mobile Phase pH as a Method Development Tool

The use of Mobile Phase pH as a Method Development Tool

[@prasannakumar1614]

Only the master can make tough concepts to very understandable way... u r my master, bhaskar sir

[@rahulkulkarni3037]

Beautifully explained importants of pka vs pH and their retention in RP-HPLC thank you Sir

[@bhawanibanerjee1214]

Thank you for this, very helpful for method development. Cheers!

[@syedrashedfaizan5990]

Simply and easy explanation, thank you for sharing such awesome content.

[@raju8184]

Excellent explanation, keep doing good work

[@riyazdantroliya3711]

Valuable knowledge , thanks sir

[@rajujonnada8987]

nice information...its useful to pharma analysts

[@Learnwithsimon27]

Really good content.

[@sanavadapankaj18]

Good Sir & Thank u ...

[@siddhachavan8604]

Nice and simplest explain

[@venkateshsanthanam100]

Thanks sir Nice explained sir

[@lellanarendrakumar6831]

Thank you❤

[@user-yi7kj8ih8f]

Thank you sir

[@jaykumarbhatt8841]

Very nice 👌

[@bestdancevideos2253]

Superb Superb Superb Superb Superb Sir

[@sachintadge8124]

Nice sir..

[@thehealer8330]

If sample contain more than 1 analyte I.e 2 or 3. How pka value and pH Mobil phase calculate??

[@azhar17kkk]

Thanks Sir, it will be just opposite in normal phase column right? Please correct me if I'm wrong.

[@seyedmohammadseyedkhademi2454]

Very good please go on about troubleshooting of HPLC

[@nguyenhoaiphu]

Could you tell me the app you use to draff, sir?

[@buddepumohanarao8309]

Sir please make video on HPLC column selecting

[@prasannakumar1614]

Sir, if our compound having acidic and basic both, then how will manage the mobile phase pH.. please reply

[@sunandasolomon6406]

PKa of my compound is 7.6. What should be the pH of my mobile phaase?

[@parmardevendra4056]

Sir, if both drug pka I'd 8.71 & 12.49 so, how can I select mobile phase.

[@mayurib5186]

Compound remains in which state while retaining on column? Also plz make a vedio on behaviour of zwitterion in chromatography. How does it behave?

[@sanavadapankaj18]

If pka is 17 , what should I have to do.

[@atozfacts9403]

What a wonderful presentation it is, which I have never been gone through. how do we know the pka of new compound?

[@pharmagrowthhub3083]

Thank u for your comment. watch my video for pka measurement.

[@anantdhopat9928]

Excellent sir,make more videos on usp general chapters

[@sachintadge8124]

Right...please make video sir

[@apnacoffeebreak7427]

Sir, what impact will be there on LC if Mobile phase's pka and Compound's Pka is same.?

[@TheChamp91100]

PEAK BROADENING

[@tadalavenkateswararao2090]

Sir how to know compound acidc or base

[@thanishasayyad2845]

But, acidic compounds retained in acidic pH and basic compounds retained in basic pH.

[@prasannakumar1614]

Yes, that's not correct. At acidic pH, basic compounds tend to undergo ionization, releasing ions and becoming more soluble in water. The specific behavior can depend on the nature of the compound and the pH conditions.

[@vinodsingh700]

Sir what about neutal molecules?

[@sanavadapankaj18]

Neutral molecule not affect ed by ph change

[@bestdancevideos2253]

I Want column chemistry

[@bestdancevideos2253]

Why not Upload new Videos Sir

[@elumalaitamil3230]

Dear sir, I have one doubt in analytical method validation. When the monograph method is used for validation in compound having more impurities and some of impurity (two or three) elutes same RT how to arrive RRF in this case further monograph mentioned RRF is 1. Please suggest the way forward 🙏

[@bestdancevideos2253]

Why To Not Updated Any Videos

[@pharmagrowthhub3083]

We will have few very soon...