Phenol chloroform DNA extraction method

[@acebiosciences108]

Past 7 months ago while coming for the interview i revised from this Vedio. I was able to recall everything. There was question in the interview from. Currently selected and working 😎.

[@manishamalhotra1612]

I am really happy for you. Keep going and keep growing 💎

[@acebiosciences108]

@@manishamalhotra1612 tysm currently working as researcher in ELISA & VIROLOGY DIVISION, ADRI, CUTTACK . I hope I'll get benefitted from your channel for NET / GATE too . Keep growing 🙏

[@manishamalhotra1612]

All the very best for your future endeavours

[@acebiosciences108]

@@manishamalhotra1612 thanks a ton

[@salmansiddiqui6586]

Awesome explanation, Nice and humble voice. Love you Mam❤💚💙💜

[@gaziilyas8865]

Your teaching level is outstanding mam

[@killerqueen8838]

thank u so much for this video ! I only have one question, chloroform is in the organic phase right? along with phenol of course

[@ven-lauraatem7331]

Thank you so much ma'ame. This video is clear and concise, loved it. However, I wish to inquire which tool was used in curating the workflow diagram?

[@manishamalhotra1612]

You can use biorender

[@Bb_xvii]

Thanks you Mam your explanation is outstanding and it helps a lot for theory

[@manishamalhotra1612]

Thanks happy to help

[@zaturitzeik1055]

Maam, can you please explain, what happens to RNA during the extraction procedure. Doesn't it contaminate DNA?.

[@manishamalhotra1612]

At the end when you resuspend the DNA in TE buffer you add RNase along with it so that it will not affect the yield of DNA

[@mastersacademy5393]

It is very helpful. thanks a lot, Ma'am

[@syed-wd8ib]

How MgCl2 only interacts with negatively charged lipoproteins but not with the phosphates of DNA backbone,

[@nazrulislam-ol8wt]

Very beautifully explained .

[@riajulislam6891]

Please ma'am, provide me the protocol as pdf format🙏

[@sadaftagar220]

mam quanitity of any thing is not mentioned? Like how much amount of TE, Isolpropropanol and other chemcials should we add?

[@manishamalhotra1612]

It's depends on the amount of inoculum taken and regarding CTAB it's basically used to isolate DNA from plants tissues

[@fatmaelgedawy2061]

Please I need a lecture about sapoviruses and safffold

[@bantybaghel264]

Hello mam, What method gives best result for DNA extraction , DNA Extraction kit or phenol chloroform dna extraction

[@manishamalhotra1612]

Kits are good, but i always prefer the classic method of phenol chloroform and i get good results

[@abdullahshaon7192]

Can we use the same chemical for extracting genomic DNA from animal blood or need some more chemical?

[@manishamalhotra1612]

Yes but the pH of the salt varies with it for plasmid we generally use acidic pH but for genomic DNA the pH is close to neutral

[@bhagatsingh6345]

Ma'am which method use for fungal DNA isolation. Please can you provide the protocol. Wait for positive response.

[@manishamalhotra1612]

Mail me at [e-mail]

[@sadaftagar220]

can we use CTAB instead of SDS

[@agnespa5242]

Ma'am can u please provide the name of the reference material ..

[@kaian2980]

I hope my teacher would teach like this. She just gives us the protocol and let us be. :((

[@manishamalhotra1612]

M glad it's helpful.

[@vishalrathod7342]

Thank you mam I am msc horticulture student I am working on molecular biology

[@manishamalhotra1612]

Hope this is helpful..

[@shortbee6813]

Thank You so much !

[@AkbarKhan-wx3yo]

please kindly give information on how to extract from animal tissue

[@thiwankaperera8603]

Well explained

[@studywell4480]

can u explain plant dna isolation using phenol chloroform method

[@namrtasharma1842]

WELL EXPLAINED

[@shivaniojha1347]

Mam how to precipitate rna while purifying dna

[@manishamalhotra1612]

If you want to remove RNA you can add RNase in ur protocol

[@yuenme5612]

@@manishamalhotra1612can I ask, in which step we can put in RNase if we want to precipitate rna (based on procedure in yr video)

[@AhsanUllah-ie8ml]

Out standing 👍

[@aenaaye]

thank you so much

[@GaneshKunayak]

Excellent 🔥❤️

[@manishamalhotra1612]

Thanks😊

[@misbahpathan58]

thanks a lot

[@hafsashaukat3709]

perfect mamm

[@yousufkhant.l2440]

good lectture mam

[@ankurtripathi1633]

I should have taken biology I think 🤔😛

[@soumayaelhamdouni4479]

❤❤