Plant Pigments, Chromatography

[@jesselmore4780]

“Stop Richard”

[@Hala_Alshararii]

A small correction here though, at 8:15, the RF value must be smaller than one, not zero 👍

[@lindseymao8961]

Thanks. I'm using this during the COVID19 quarantine for my class

[@biologypracticalsandrevisi8066]

Lindsey Mao Me too. Glad it’s useful.

[@sanahafeez859]

Please make some more videos on the experiments of A2. Your videos are really helpful

[@aaronfogg8746]

8:15 Smh "A value of less than zero"

[@kidodakid8861]

Lol

[@neammoore5083]

Thank you I did a test with a 6 mark question about this experiment they did in class but i was out of lesson so got not enough marks and now resitting test

[@rishikavashishtha6556]

Thank you so much.... Really saved me big time

[@LannieCamper]

you've saved my alevel thank you so much

[@mylifestyleat2030]

Yes u r absolutely true.

[@happyhanabi1632]

What is the purpose of using sand?

[@biologypracticalsandrevisi8066]

Hanna Bouftah it’s gritty texture just helps to break up the lead tissue.

[@happyhanabi1632]

Biology Practicals and Revision Biology Tutor thank you

[@justinbieberfanforever2786]

Thanks thanks a lot because of you I learn this experiment tomorrow is my intermediate practical exam

[@annasimone7780]

Does the solvent have a collective name?

[@umar757]

omg I found this so late

[@memelator]

When grinding the leaf and sand and acetone...what is the liquid called that is produced?

[@leoneyssymon4299]

Very interesting thank you

[@tulsichhetri2554]

Thank you, it is a very informative video indeed. But is there really no substitute for TLC paper that can be used?

[@appandairajan7246]

Instead of alumina plate, one can use silica gel plate that can be made from silica gel added with solvent and poured on a glass plate, leveled and kept in oven.. silica gel plate ready to use now

[@manimadala6119]

Ohh.. thank you so much 👏👏👏👏

[@susmitamondal4795]

Thank you.

[@hafeezullah3641]

Nice work

[@brianzhi2815]

so you don't need to strip the solvent from the pigments?

[@Idk-ij1yy]

Is there any way to improve this method

[@chingandrea8206]

What is the concentration of the acetone?

[@eden-mariederosaissott1324]

what does the propanone do?

[@biologypracticalsandrevisi8066]

Spoken word Spoken from the heart it’s is a good solvent for some of the compounds in the leaf.

[@kokokoko6787]

Thenk you

[@mhafiz4719]

why did you add acetone during the extraction ?

[@biologypracticalsandrevisi8066]

Nurarihyon hafiz it dissolves the phospholipid membrane so that more pigment is released and acts as a solvent for the pigment.

[@mhafiz4719]

@@biologypracticalsandrevisi8066 Ohhh I see.. Thank you

[@africanqueen9820]

Can u use water to separate the colors 🤔 I'm doing a PD lab for the first time

[@biologypracticalsandrevisi8066]

kaylia walker You could try it. You are unlikely to get a good separation but you should get something.

[@africanqueen9820]

@@biologypracticalsandrevisi8066 but so I think I should try using the distilled water and the alcohol

[@martajackson11]

@@africanqueen9820 Hi biology student here. From what I know, you can't use distilled water or even water to separate the pigments since the pigments are located in the thylakoid membrane inside the chloroplast, which means we are dealing with a membrane and membranes usually have a lipid constitution and if you want to 'break' the membrane to dissolve the pigments, you absolutely need an organic solvent, which is why you use acetone and petroleum ether. Please correct me if I am wrong.

[@africanqueen9820]

@@martajackson11 thank you very much

[@theo9743]

where did you get the rf values from?

[@biologypracticalsandrevisi8066]

Theo You calculate it and compare to standard values for pure pigments.

[@theo9743]

@@biologypracticalsandrevisi8066 Sorry for the misunderstanding! I meant the source of the pre-determined Rf values that you compare your results to!

[@biologypracticalsandrevisi8066]

Theo there are a few txt books with them in or you can run pure pigments yourself with the same solvent .ill see if I can send you a link to them.

[@theo9743]

@@biologypracticalsandrevisi8066 Thanks!

[@tergelmaasukhbaatar8331]

Why would you use a sand and plant to grind the mixture

[@biologypracticalsandrevisi8066]

The rough texture of the sand helps to break apart the plant tissue.

[@tergelmaasukhbaatar8331]

@@biologypracticalsandrevisi8066 thanks

[@jefflelemon3539]

Does anyone know why you use a mixture of solvents instead of just propanone

[@biologypracticalsandrevisi8066]

The different pigments have different solubilities in the various components of the solvent. The mixture gives better separation.

[@jefflelemon3539]

@@biologypracticalsandrevisi8066 Thank you very much! :)

[@alamsquadri1021]

thank you too much mam

[@andrewsummer3521]

Can you please say the exact pigment distance and solvent distances?

[@biologypracticalsandrevisi8066]

Sorry I can’t help with that. It was a while ago and I don’t have the raw results. The distances will change depending on the type of stationary phase and exact composition of solvent used.

[@RachelJ715]

It would be helpful if you explained WHY you're doing what you're doing and not just describe it

[@neammoore5083]

Rachel Munday to find the rf value

[@mimmovasta1390]

guzzo è un sottosviluppato

[@auroradeluca3898]

Ma cosa 😂😂😂

[@saratarantino3409]

@@auroradeluca3898 ciao a tutti figaaaaaaaaaaaaaa

[@auroradeluca3898]

@@saratarantino3409 stiamo invadendo pure you tube

[@saratarantino3409]

@@auroradeluca3898 infatti ;)

[@jasminewallace-thompson524]

Why must it be a pencil line and not a pen line

[@biologypracticalsandrevisi8066]

Jasmine Wallace-Thompson if you use pen, the ink will dissolve in the solvent and run up the paper obscuring the results.

[@jasminewallace-thompson524]

Biology Practicals and Revision Biology Tutor thank you so much! Another question why must the pigment spot be above the solvent surface?

[@biologypracticalsandrevisi8066]

Jasmine Wallace-Thompson if it wasn’t it would dissolve in the solvent rather than being carried up the paper by the solvent.

[@JamieMartinSermon]

"Stop Richard" 8:29

[@bapujim]

One should never disturb the jar while the plate is running. Your resolution.is very poor. Please improve solvent system.

[@shushhhhh1983]

Mr kirsten man allow it

[@fozzzzzil]

Stop zooming already

[@ronaldchau9723]

stop it char.......

[@bapujim]

Very poor handling dear. You should never touch the surface of tlc plate. The plate width should be such that it would not touch the sides of the jar. The edges of your tlc plate is very badly handled. Please donot mind. Young people should learn better handling.