2.9 Separation of Photosynthetic Pigments by Chromatography (Practical 4)

[@rondayaram5792]

Incredibly detailed. Well done. Thanks for sharing

[@am17.07]

Absolutely helpful and on the point. Thanks. My lab practical is on it's way. It helped a lot

[@idkhahahaha]

but what does the Rf value actually tell you about the pigment?

[@nguyenkienam2331]

this helped me a lot in lab mixture separation report

[@37KA4]

Hi please can I contact you via email ..

[@Kwork1104]

Helpful video Must all bsc students watch this before exam❤❤

[@breeze3430]

Thank you! This is a helpful guide for our Plant physiology laboratory activity

[@teacherjayzeelynvanessacor6889]

9 ⁹k

[@drewhenderson8223]

Your welcome

[@DeepikaSharma-io7bl]

Well done mam it's really very helpful video for us keep create video for us

[@phenyotshabang6394]

Can someone please explain why acid washed sand and anhydrous MgSO4 are used in the preparation of the extract of this kind of chromatography.

[@hotpepper7782]

Well my bio teacher said the acid washed sand was just there to make the grinding process easier about the anhydrous MgSO4 idk y it's there.

[@deminigamage9438]

Thank you so much! I didn't understand anything given in my lab manual. But this is very clear.

[@kennyyong7694]

Excuse me, it is the same procedure for thin layer chromatography right?

[@Palak..15]

Yup:)

[@peneloppedjeakoua1570]

Very useful for my experiment tomorrow

[@SakinaMustafa-ds8ue]

Thankyou so much for this video It really helped

[@misskhan8328]

your english is too good

[@khushiparmar7444]

Solvent system which solution use?? Can you please tell me

[@ash1ssh]

iso propyl alcohol water mixture

[@silvebullet]

this video helped me alot

[@zmazn]

what should I do or what does it mean if the carotene keeps ending up being the last one below? I tried it 2 times and its always the same and im afraid the school lab wont let me do it again and i have to make a lab report about it with my picture in it.. I used spinach leaves btw

[@ayodelebello1145]

How much acetone is used for each leaf sample?

[@detss3795]

not just acetone 5 ml solvent which contain 9 part Petroleum ether and 1 part acetone

[@detss3795]

@Elijah Tam-od Ok

[@roshnimathew3209]

Instead of ether which solvent can be used?

[@sabiha23467]

Thank you madam I understand very well

[@shristiasylum]

Good job dear👍

[@sashaart781]

Can I ask what solvent did she use? Please answer me 🙏

[@Runner12981]

Water

[@Idk-ij1yy]

Is there any way to improve this method

[@Leen-ih8ql]

what are the methods or ways of separating plant pigments?? only TLC and paper chromatography ?? please I need help

[@videoteaching47]

u can do through chemical method as well.

[@Anki1243]

Which solvent used?

[@kiransharma-oo2wq]

I think acetone

[@detss3795]

5 ml solvent 9 part petroleum and 1 part acetone sis

[@roshnimathew3209]

Instead of ether which solvent can be used?

[@evelynt.britos2863]

What’s the significance and use of Rf value?

[@evelynt.britos2863]

After determining Rf, what’s next? Does it end in finding the Rf? Thanks.

[@martajackson11]

Hi, biology student here! From what I know, the Rf value is one of the methods you have to distinguish between the several photosynthetic pigments you can find on a leaf. You can also identify the pigments just by looking at their color. Basically if you have a Rf value close to 1, that means that the pigment moved quite a lot and it was abel to do that since it a polar pigment. If on the contrary you have a low Rf value, that means that the pigment didn't move that much and thus has non polar groups in its constitution. Please correct me if I am wrong

[@xyrildanmanuel783]

hi chemistry student here. the Rf value is one of the information we use to pinpoint what the pigment might potentially be. chemists around the world do this chromatography on initially known compounds and they share and confirm the Rf values they have obtained in a database (or multiple databases like the one presented at the end of the video) so that future analysts would have a reference when repeating the same chromatography on the same compound. think of it like a pseudo-fingerprint of a certain compound although i believe not all compounds have unique Rf values

[@ahappycoder2925]

RF value is used to identify the pigment

[@jimsamson7934]

It allows you to get a measurement for 5G or 3G radiation frequencies. That way, you can gauge how thick your tinfoil needs to be in order to block those signals

[@TheHexbugfan]

Ms. Webber sends her hellos!

[@climax7260]

Thank you it helps

[@anishakumarisingh581]

Plss give me the exact amount of solvent

[@geetaikalpande8471]

AND WHAT HAS SHE ADDED???

[@niloypaul34]

thank you

[@judeigho7254]

Is the eluting solvent polar of non polar?

[@pawsitivebhumika6462]

Thanku so so much❤

[@siddikpk8385]

😊 🙏 thanks

[@daphnesaipelekomiti7254]

Can someone please my lab

[@afolabifakanbi7782]

Tanks

[@ammarsyah267]

thanks .. "a few drops of acetone" 🗿

[@keerthicreations2153]

Hii

[@keerthicreations2153]

FF

[@keerthicreations2153]

FF

[@keerthicreations2153]

FFFFFFFFFFFFFFF

[@detss3795]

@@keerthicreations2153 hi yes What u want to ask

[@randomgaming9869]

Result

[@realism6793]

wat the pigments i got 10 mins

[@ranaumararshad3351]

Tare pan nu lun