Preparing the PCR primer stock and mixes

[@markdavis5176]

ok, so my ocd showed me you touched the tip as you released it from the pipette. is a nono.

[@AmruMagdy]

Thank you, this helped me!

[@tiberium87]

Thank you, this helped me! -VS

[@zurronna]

Where's the explanation please?

[@madhans2350]

what is the concentration of mix (working solution). is it 1x or 10x

[@EelasMusic]

I have the protocols for normal PCR.how can I optimize them for using multiplex PCR

[@lunar_ascension6669]

Answer lies in the subset primers and the target sequences downstream to accomplish a multiplex pcr. qRT-PCR from PCR standard. In other words, find your target sequences and design primers accordingly.

[@oluwafemiakin-adigun8034]

100nM/L or per uL?

[@sweetymanibala3975]

Hello ma'am nice explanation of every techniques through practical way. Ma'am I have some query want to asked, how I can contact u. If possible through email. Then please reply me.

[@FabianoAPires]

VERY GOOD !!!

[@metinousidouin2953]

cool