Quantitative Polymerase Chain Reaction (qPCR)

[@guptahittu]

Thanks for sharing this wonderful presentation on qPCR. Detailed and very informative.

[@abolfazlnik-akhtar1667]

In a word: Awesome. That was awesome.

[@anndiamond7601]

Great presentation. Thank you very much for detailed explanation.

[@mehran_mansouri]

Thank you so much for sharing such a nice and comprehensive video

[@adnaniqbal4497]

EXCELLENT . VERY WELL EXPLLAINED. CLEARED ALL MY CONFUSION. THANKYOU SO MUCH

[@johnjakewinkler7484]

Great video...but qPCR is not limited to quantifying the cellular expression of mRNA.

[@YuetongUBC]

Thank you very much for your video! a small question: Is it possible to do the mRNA with a small sample size (e.g. 100,000 cells) for non-housekeeping genes?

[@sylvesteraddai-arhin5098]

Great presentation, and very explanatory. Thank you!

[@hystrionic]

i'm interested in the info but the vocal fry is making it hard to listen.

[@stevebow7337]

Great presentation. Thank you so much for such a detailed explanation and also your personal suggestions from your real experiments. It really helps me in setting up my experiments and knowing what to do.

[@samihahzuramohdnani4137]

Is it 1:10 working primer dilution right? Should it be ddH20 - 180 ul

[@mohi7527]

Excellent presentation, thank you! But all this explanation is about qRT-PCR and not qPCR. Am I right?

[@chrisjmolina]

Thank you for your excellent videos!

[@nizamuddinbulbul5942]

Clearly explained....Thank you!

[@dopefish3937]

Great presentation, thank you! But I wanna ask qPCR introduced in your video means the Reverse Transcription-qPCR, right? However, quantitative Real-Time PCR is also called qPCR. Actually, quantitative Real-Time PCR and Reverse Transcription- qPCR are different, which is because the former doesn't involve RNA extraction and reverse transcription, but only DNA amplification. Generally, Reverse Transcription-qPCR maybe more often be named as RT-qPCR or RT-PCR.

[@prof.dr.mohamedezzat8227]

RT-qPCR or RT-PCR are not the same. RT-PCR is a conventional PCR but includes an RT step before the PCR, while RTqPCR is a real time (either quantitative or qualitative) PCR including an RT step either a one step reaction or a two step reaction, therefore when talking about real time, the standard abbreviation is q and not RT.

[@gehadmahmoud6058]

Very informative. Thank you

[@jangidmanish6244]

Thanks... Great tutorial

[@romarichippolyteagassin3463]

We need to design a specific qPCR primers of the gene or we can also used the primers designed for common pcr?

[@ahmed-lab]

use this tool to design your qPCR primer www.genscript.com/tools/real-time-pcr-taqman-primer-design-tool?src=google&gclid=Cj0KCQiA8aOeBhCWARIsANRFrQGM38dfc7LgVjoEkIVsjGq5-PZACD6BPEfMpYtlv1sBrioS_xZpK9saApwjEALw_wcB

[@romarichippolyteagassin3463]

@@ahmed-labThank you very much. I appreciate 🙏

[@hammedtukur]

Excellent

[@neshatmd6924]

great ! thanks!

[@osmirod1]

You’re a god !

[@walkerchen7336]

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[@samuel_prince]

14x 🤔🤔