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How a gene gun works

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TAXA Biotechnologies

TAXA Biotechnologies

Күн бұрын

Kyle Taylor from the Glowing Plant project explains how a gene gun, which is used to transform cells with synthetic DNA, works.

Пікірлер: 55
@JulianoVitiello
@JulianoVitiello 11 жыл бұрын
Here is a good tip: wrap electric tape on the top part where you screw in the rupture discs. That way it wont sound like nails on a chalkboard each time.
@mewantbrains
@mewantbrains 9 жыл бұрын
I believe in the video you said you will demonstrate it firing I don't see that anywhere in the video
@DJMouthwash
@DJMouthwash 9 жыл бұрын
***** 0:28 seconds.
@mewantbrains
@mewantbrains 9 жыл бұрын
That's where he says he will demonstrate the firing process but it did not actually fire
@TAXABiotechnologies
@TAXABiotechnologies 8 жыл бұрын
It does fire, it's just not that dramatic!
@ashdroid
@ashdroid 7 жыл бұрын
The machine is turned off and not running at that specific time 0:28, it even has the door open. EDIT: I just saw your other video.
@abefromansausagekingofchic6480
@abefromansausagekingofchic6480 6 жыл бұрын
Riddlemethis yea I was kind of blue balled too. Found the other video though
@lakshayaindrapugal542
@lakshayaindrapugal542 Жыл бұрын
Thank you soo much. Actually I was thinking how does this thing work?. It was referred as " bombarded " in my book 😂.
@anushankar8364
@anushankar8364 11 ай бұрын
Neet aspirant things😅
@devikadevuz4612
@devikadevuz4612 10 ай бұрын
NCERT😂🤍
@birk_lab4805
@birk_lab4805 5 жыл бұрын
You stopped before the bombardment! WHHHHYYYYY??? :,,-(
@realryder2626
@realryder2626 Жыл бұрын
Obviously he got a txt and had to reply
@DipanshuGhosh
@DipanshuGhosh 4 жыл бұрын
You should have demonstrated the whole process. You didn't fire the nano particle coated with preferred DNA.
@lolpotato
@lolpotato Жыл бұрын
Generally gold or tungsten with attached desired gene is used to fire into the vector. This is what i have been taught but never saw it in practical
@Noveshd
@Noveshd Жыл бұрын
​@@lolpotato yep its only done when u choose biotechnology as career.....not in medical also
@adventuresoflife486
@adventuresoflife486 2 жыл бұрын
If there is no vector present we use Gene gun/bolistic for plant and microinjection for amimal... 🌷❤️❤️
@callahancovington4278
@callahancovington4278 8 жыл бұрын
It would be nice if there was a laser to help you align the tissue directly into the gun's trajectory.
@blazearmoru
@blazearmoru 11 жыл бұрын
aww. you didn't show us the process with the onion D: i hope it's in the next video ^~^
@theashmedai007
@theashmedai007 6 жыл бұрын
Can u combine mamels with plants
@KaitokiNohara
@KaitokiNohara 4 жыл бұрын
Now I know how it works
@PrasetyoMuhammadDwiBiology
@PrasetyoMuhammadDwiBiology 7 жыл бұрын
real men use heatshock :D
@kellycarpenter9350
@kellycarpenter9350 3 жыл бұрын
Hey I would like to learn how to inject flavonoids into plant tissue to create flaver into plants .like strawberries..I want to cross flaver with pink pepper etc. Kel
@jazzmessenger8
@jazzmessenger8 10 жыл бұрын
What genes were on the tungsten?
@jayesper4390
@jayesper4390 6 жыл бұрын
Bill Ricci Dunno, but gold's the way to go. Tungsten should be out of the question.
@NavinJohnson_thethird
@NavinJohnson_thethird 3 жыл бұрын
Dr. John Sanford waa the primary inventor of the gene gun. A former Atheist who became a Christian.
@David-ck3gv
@David-ck3gv 2 жыл бұрын
The prototype was also just a .22 caliber that shot blanks into plant tissue lol
@Ekergaard
@Ekergaard 11 жыл бұрын
I have heard a Ph.D. student say: "This is our nanodrop there're many like it but this one is our". So it's temting to ask if anyone has said: "this is our gene gun ..."
@sapnamenghwar8219
@sapnamenghwar8219 Жыл бұрын
Oh I was waiting for them to bombard the tissue, just to see the machine in action 🤔
@deborahjudson350
@deborahjudson350 7 жыл бұрын
It does not ever fire
@FoxCurtailed
@FoxCurtailed 11 жыл бұрын
It looks like a tiny, old school gas pump.
@axanachernyavskaya3556
@axanachernyavskaya3556 10 жыл бұрын
thanks a lot! very useful!
@zombiebillofthedead
@zombiebillofthedead 11 жыл бұрын
Filming on a phone I see, wouldn't have known if the picture didn't shake and make that vibrating phone sound. Not that it matters.
@smilesinger3652
@smilesinger3652 3 жыл бұрын
Guys who are from vyshavi college 😀
@biologyinhindiandenglish8787
@biologyinhindiandenglish8787 4 жыл бұрын
Ho sakta you can follow any responses to you and
@christurnblom4825
@christurnblom4825 6 жыл бұрын
politics of gmo vs non gmo aside, I find it humorous that I've actually heard a scientist on the radio say that this is no different than what farmers have done for thousands of years when breading plants for a desired outcome. This, in response to being asked what he has to say to people who are concerned about the safety of gmo's. They weren't taking calls but if they had been my question would have been "What force of nature causes a similar effect to a gene gun and achieves the same result?" Whether or not there is net harm to the environment aside, I just found it insulting that he would play on people's ignorance like that.
@christurnblom4825
@christurnblom4825 6 жыл бұрын
So one of those you mentioned, blasts the same "clip" of DNA into other DNA at over 600 mph, blasting apart the DNA while getting random pieces to stick in the desired place? I guess I do have a lot to learn.
@christurnblom4825
@christurnblom4825 6 жыл бұрын
What are you talking about? Sarcasm is great! Example: Damn you for knowing stuff & making me think about what I've said!! Sarcasm helps me feel like I'm doing well while your schooling me. ...and it's occasionally funny. All I got left is the thing I put aside at first ie my concern that being selective in that process has a different outcome than in nature or even when we selectively breed in the old method because we don't have all the other iterations that would result, out their doing their thing. I doubt we have any tech. that can figure out if those two ways are any more or less harmful to the environment than the gene gun method but my hunch is that it probably puts things out of balance. At the same time, I'm pretty sure that nature will just shake us off or swat us with her tail and go back into balance if we become too much of a problem.
@FringeWizard2
@FringeWizard2 6 жыл бұрын
I don't think there is a problem with genetically modifying organisms but the specific traits they choose to develop in plants are often really stupid traits they should not have aimed for, whether it be through selective breeding or GMO. Stuff like tomatos that produce more crop with the same amount of nutrients; the result is tomatoes that have significantly less nutritional value. GMOs plants the produce their own pesticide are also a stupid "innovation" as pesticides are often (not always) not very good and harm our health. When they try to develop plants with commercial interests in mind it often times has a lot of drawbacks. It's not also just GMO that is bad but many varieties of plant which are grown for commercial production are in my opinion inferior to heirloom varieties developed by gardeners who just wanted a tasty or beautiful tomato.
@selenaburke2956
@selenaburke2956 6 жыл бұрын
There are foods you eat with transgenes that got there naturally. like potatoes
@MinxInSinx
@MinxInSinx 2 жыл бұрын
@@FringeWizard2 do you think it is ok to genetically modify your genetics & the lineages of your offspring? The only way for your dna to change is if you inject it into the user. We are what we eat
@ducktheduck9792
@ducktheduck9792 3 жыл бұрын
kuku
@user-vw9jl9du9y
@user-vw9jl9du9y 3 жыл бұрын
الي ما يعرف انكلش شلون يتابعة🥺
@balbaks
@balbaks 3 жыл бұрын
بتقدري تدوسي على علامة CC وبعدين روحي اعدادات الفيديو > الترجمات > ترجمة تلقائية، هي فيها شوية تخبيص بس راح تساعد بالفهم
@user-vw9jl9du9y
@user-vw9jl9du9y 3 жыл бұрын
@@balbaks تطلعلي كتابه انكلش ما يصير عربي ؟
@balbaks
@balbaks 3 жыл бұрын
@@user-vw9jl9du9y لا موجود عربي بس ترجمة تلقائية بعد ما تدوسي على CC روحي لاعدادات الفيديو (إشارة المسنن) واختاري ترجمة تلقائية أنا فاتح من الحاسوب ممكن من الهاتف يكون مختلف
@user-vw9jl9du9y
@user-vw9jl9du9y 3 жыл бұрын
@@balbaks يمكن الموبايل يختلف راح اجرب الحاسوب شكرا جزيلا الك
@timdwyer3516
@timdwyer3516 6 жыл бұрын
Anyone here from 7/8 b
@anusandhanwithdevantiwari181
@anusandhanwithdevantiwari181 4 жыл бұрын
Can u tell me hindi
@user-nr6kc2ie4k
@user-nr6kc2ie4k Жыл бұрын
PDS1000$$
@miguelpatrao8999
@miguelpatrao8999 2 жыл бұрын
ahhhhhhhhhhh cromo
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