Recombinant DNA technology | NMDCAT 2021

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About This Video ........................
In This Video Lecture You Will Learn Basic Concept Of Recombinant DNA Technology
Recombinant DNA technology popularly known as genetic engineering aims at
1. Gene of interest, which is to be cloned.
3. Molecular carrier or vector, on which gene of interest could be placed.
4. The gene of interest along with the vector is then introduced into an expression
order to produce recombinant DNA, the following are required:
synthesizing recombinant DNA which contains DNA from two different sources. In
2. Molecular scissors to cut out the gene of interest.
system, as a result of which a specific product is made.
How to get a gene?
cut the DNA at very speciic sites characterized by speciic sequence of four or six
nucleotides arranged symmetrically in the reverse order. Such sequences are known
which a piece of foreign DNA can be placed, if it ends in bases complementary to those
Another very common method of getting the gene is to synthesize it in the laboratory
growth of viruses. In 1970, Hamilton O. Smith, at Johns Hopkins University, isolated the
as palindromic sequences. So far more than 400 such enzymes have been isolated out
If, however, the genes are small, they can also be synthesized in the laboratory.
Genes can be isolated from the chromosomes by cutting the chromosomes on the
EcoRl, a commonly used restriction enzyme, cuts double-stranded DNA when it has
exposed by the restriction enzyme. The single stranded but complementary ends of the
complementary DNA (cDNA).
lanking sites of the gene using special enzymes known as restriction endonucleases.
(b) to synthesize it chemically, and
These are natural enzymes of bacteria, which they use for their own protection against
(c) to make it from mRNA
viruses. The restriction enzyme cuts down the viral DNA, but does no harm to the
of which about 20 are frequently used in recombinant DNA technology.
this sequence of bases at the cleavage site . Notice there is now a gap into
bacterial chromosome. They are called restriction enzymes because they restrict the
(a) to isolate it from the chromosome
Molecular Scissors: Restriction Endonucleases
from messenger RNA, using reverse transcriptase. This DNA molecule is called
There are three possible ways to get the gene of interest.
two DNA molecules are called “sticky ends” because they can bind by complementary
irst restriction enzyme. Bacteria produce a variety of such restriction enzymes, which
base pairing. They, therefore, facilitate the insertion of foreign DNA into vector DNA.
Molecular Carrier: Vector
antibiotic resistance gene for tetracycline, whereas pBR 322 has antibiotic resistance
To make recombinant DNA, one often begins by selecting a vector, the means by
antibiotic resistance and fertility etc. One of the plasmids discovered earlier pSC 101 has
which recombinant DNA is introduced into a host cell. One common type of vector
is a plasmid. Plasmids were discovered by investigators studying the sex life of the
Plasmids are natural extra-chromosomal circular DNA molecules which carry genes for
intestinal bacterium Escherichia coli.
genes for tetracycline as well as ampicillin. Inserting gene of interest in tetracycline
resistant gene of plasmid pBR 322 would enable separating out colonies of bacteria in
a medium containing ampicillin and vice versa.
About This Channel.....................
I make these videos cause I love to draw and connect the complexity of science and medicine into art. I'm not saying. I'm 100% correct in all my videos, but I do try to obtain the information from credible sources.
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_ students in class 11, 12 or appearing for competitive medical exams ( NMDCAT ) will find it very beneficial
_ The videos are categories under various heading to make the students life simplified
Visit My Other Channel Playlist .....................
NMDCAT PART 1: • New 1st Year Biology
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ranaalibiologis... TWINS

[@areebaanwar3352]

sir Im really thankful to you for improving my biology. biology was my weakest subject but now Im getting better as i am taking your lectures and making notes from your lecture I just have one request ... can you please post biology chapter 23 biotechnology class 12 as soon as possible as you know that the board examination are around the corner.

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Sir G PSC 101 Is for tetracycline not for Ampicilline.... Mistakenly u have Written that.

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Sir psc 101 for tetracycline h ampicilin nai

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sir psc 101 have only tetracycline gene not a ampicillin....... ye book me mention hai per video mey by mistake sir se ghalat ho gaya 🙃

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@@ALIACADEMYAR7 sir Fsc 2nd year chapter 23 biotechnology ki playlist nai mil rhi please guide

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Uploaded nhi ha

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Sir it's an emergency work please share a video on Identifying disease genes in genetic Please sir I am not getting the main idea of this topic

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Restriction endonuclease enzyme

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May be 👍

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Psc 101 contain tetracycline not ampicillin 😢

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Cloning

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