Retrovirus mediated Gene transfer | RMGT | procedure | Bio science

[@Uncover123]

Thanks a lot sir ❤ tomorrow is my exam and now i think confident enough to answer these question😊

[@mudigondasreelatha7812]

👌👌👌👌👌👌 annaya..we want more videos for CSIR NET topics please annaya

[@RG-Gamer-Ayush]

Thank u mam luv from Jammu and Kashmir..

[@vaidyarohan1709]

Such a simple and detailed explanation loved it

[@ashishpaul4251]

Thank you so much sir for such a helpful information ❤❤

[@nanditabanerjee2407]

Thank you .. this video help me a lot .. really thank you🤗🤗

[@bioscience6853]

My pleasure

[@khwaishyadav2593]

Rat nhi mice....thanks for the explanation 🙇

[@bushrarasheed2001]

This is indeed helpful! 👍🏻

[@vaguelyf]

The part that I find weird is how inserting host bacterial cells into embryo will initiate expression of desired gene in the progeny. Because the embryo already has it's own genome. If you can please explain...

[@musharraf7507]

Very helpful !❤

[@youthink7978]

How would you join the sequence of RNA with the help of DNA ligases 🤔

[@nitimohan3896]

Thank youu so much sir your videos are very helpfulll 😊

[@bioscience6853]

My pleasure

[@shailajasha7391]

Simple and easy 💓

[@rakshitagaddam3058]

Hybridoma technology and in vitro in vivo fertilization kuda explain cheyara sir

[@tejaswinirajeshovecreation8413]

Superb ga cheparu sir

[@bioscience6853]

Thanks

[@tejaswinirajeshovecreation8413]

@@bioscience6853 sir set syllabus topics explain cheyandii sir

[@xyzxviper6812]

Sir notes v banake dena Bohot kssstttt hua Cc on karke notes banate

[@kakarsabir9198]

DNA of virus having desired gene will also have their own gene which might be lethal for host(embryo) .. these lethal gene will not have undesire affect on host(embryo)????

[@straight652]

Sir please make a video on axis formation in zebra fish.

[@chandanamc2162]

Ulmately explaining tq

[@bioscience6853]

My pleasure

[@betneha]

thank you

[@ayansingharoy8855]

How host cell inoculate into bacterial cell culture in step 5?

[@devipriyapalanivel2518]

You are awesome 👍

[@guy_from_jammu]

thanku nice explanation

[@bioscience6853]

My pleasure

[@suhani8145]

Worthy afford !!!✌️.......

[@rajanmoogi9466]

Did u introduce host cells (bacterial culture) into the embryo?

[@khwaishyadav2593]

Yes

[@akankshadekate6904]

well explained

[@rakshitagaddam3058]

Retroviral vector method ana RMGT method ana same ana annayya

[@cakebunny2022]

awesome explaination

[@bioscience6853]

Thanks

[@jainendrajha11]

Thank you so much sir🙏

[@bioscience6853]

My pleasure

[@dharasolanki1142]

can we use this tech. in plants also?

[@kalaiselvir9389]

No..

[@dharasolanki1142]

@@kalaiselvir9389 ok thank you

[@aishwaryaudhayakumar7339]

Upload vedio on hybridization technique :probes (radioactive and non radioactive ), detection plzzz bro... upload soon

[@bioscience6853]

Sure dont worry!! ☺

[@aishwaryaudhayakumar7339]

@@bioscience6853 okay bro upload as soon as possible and next vedio indhadhan irukum nu namburen bro❣️👍

[@mrunalijagatap1579]

Thank u sir ...well explained

[@bioscience6853]

My pleasure

[@timothy8048]

Thanks bhai ❤️

[@shresthaswain9103]

Thank uhh...

[@bioscience6853]

My pleasure

[@areebamubeenashraf9571]

Good effort....plz can u tell me drawbacks of this technique.?

[@anshumansingh9132]

Bravo

[@palwinderSingh-mg6ni]

Thank you sir 😍

[@bioscience6853]

My pleasure

[@karthikeyan-sl1ek]

Well explained

[@bioscience6853]

🙂🙂

[@tahirsmedicalandbiologyvid4969]

is the restrictions enzymes also cuts the RNA? because dna and rna nucleotides are different and how they cut RNA also?//

[@tahirsmedicalandbiologyvid4969]

thanks

[@shazialone7545]

Thanks a lot sir

[@bioscience6853]

🙂🙂

[@sneka.m7768]

Thanking you broo🙏🙏

[@bioscience6853]

My pleasure

[@AS-uf3lp]

Easy👍👍👍👍👍🙏🙏🙏🙏

[@asiakhatoon1079]

Thank you sir

[@bioscience6853]

My pleasure

[@harshbaid2404]

Target gene is a dna fragment and the retrovirus genetic material is rna based. how can we intergrate a dna fragment into an rna genome?

[@vaguelyf]

Either the retrovirus has the reverse transcriptase enzyme or we provide it to convert the rna into dsDNA, then the integration process follows.

[@khwaishyadav2593]

By the help of intergrase enzyme as shown in the video

[@arshadhussain266]

It is not replicating in RNA genome it is replicating in cytoplasm of host cell which has dna as genetic material

[@legoabhi2876]

Dude u just asked such a good question that even I forgot to use common sense 😂. The answer is very silly.

[@legoabhi2876]

When the restriction enzyme cuts the desired dna which is double stranded. It cuts only single strand. So technically u have single strand which is then ligated into viral RNA of retrovirus

[@nesad481]

Could you please update of iron absorption, transport, and storage. As soon as possible. Help me for the exam.

[@aishwarya5490]

Can u provide notes?

[@edma5686]

Hi man! i contacted u on whatsapp yesterday, i hope that you see messages ! important stuff! answer me asap

[@tahirsmedicalandbiologyvid4969]

plz must reply?

[@ForAkhirah_1]

Thankyou so much sir ♥️

[@bioscience6853]

My pleasure

[@malikrajes]

Well explained

[@bioscience6853]

Thank you