Molecular Cloning explained for Beginners

This video is a must watch for beginners to understand how molecular cloning works. All steps of a molecular cloning assay are explained here!
References:
Helpful blog article: blog.addgene.org/plasmids-101...
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Music:
Aakash Gandhi - Liquid Time (KZbin Audio Library)
Listen: • Aakash Gandhi - Liquid...
0:00 Intro
0:39 Vector generation
1:59 Insert generation
2:37 Isolation of vector and insert
3:48 Assembly
4:29 Transformation
4:51 Selection and screening
5:26 Verification
#molecularbiology #cloning #genecloning

[@NickResen]

Super glad to have found this! I did a ton of work with recombinant DNA a few years ago (since then have been on a materials science/design engineering journey) and I was looking to refresh myself on the concepts I was immersed in not too long ago. This was perfect and should honestly be shown to every fresh student in a synbio lab.

[@Austrian9876]

5:40 fyi You can also use the white-blue verification to see if the recombinant Plasmid is working. Here the MCS, the part where the DNA is inserted, lies within the promotor region of a LacZ gen, that will turn the colony blue, if the medium they grow on contains GalX. If the insertion was successful, LacZ cannot be produced, which means the E.Coli colony remains white. Sorting then, is simply scraping the white colony of and to proceed cloning with that. (Works only after you already pre-selected via Antibiotics) This is a great Video!

[@debramurray8272]

Glad this popped up just when my online students read about molecular biology. Thanks, Henrik!

[@henrikslab]

Would be glad if you share it among the students :D

[@daughteroftheking3220]

Thank you so much for all you do. Don't ever stop posting you are helping a lot of students.

[@OscarSanchez-jc1pw]

Found this as I was reviewing for my Environmental microbiology midterm thank you!!!

[@spicesmiles]

Fantastic clarification!! thank you for your help!!

[@dHeIoxy]

Great work

[@Charlieson294]

Super spannend, das ist gerade eins meiner hauptaufgaben im Labor, da ich darüber Bachelorarbeit schreibe! Wir nutzen allerdings InFusion, wofür wir keine Restriktionsseiten brauchen

[@henrikslab]

Sehr cool!

[@mostafaalsaw9771]

WOW It's not easy to explain this in 6 minutes.

[@Logan-rj4he]

"Promosm"

[@user-xi9gs2nu9c]

Can you believe it tho This is being taught to a grade 7

[@user-di5zv3in4d]

Thank you. Good explanation