Pacific Biosciences Sequencing

[@jaredbernard1582]

You're great at organizing and presenting this material. Honestly, I'm even more impressed that you can seamlessly write backwards and inverted! No wonder you're good at teaching transcription!

[@christopherhall3645]

Surely this is sarcastic? Why would they not just invert the image haha.

[@mayascull9857]

@@christopherhall3645 Thankyou for explaining this! I feel so dumb hahaha

[@habeebshaik6966]

Such a need and organized presentation. Great composure. Thank you.

[@she_is_a_hungry_raccoon]

GOD BLESS YOU AND ALL YOUR FAMILY SIR

[@nom3nnescio]

this is science, take your mumbojumbo nonsense away from here

@@nom3nnescio can you explain to me where the laws of nature come from?

[@nom3nnescio]

@ by observing what we see/measure of course. Have you ever heard of this thing called science?

[@nom3nnescio]

@ or do you think your god is real? lol, it's not. It's just your imagination and you probably learned it from your parents. There is milloins of gods recorded, why only yours (out of millions) is the only and real one? Why don't you believe in other ones?

@@nom3nnescio laws are abstraction of patterns we see in the universe. what i meant was: what makes the universe be ordered, rather than chaotic? Where did the universe come from?

[@loljoker127]

Really well put, thank you making it so visual too!

[@jkuo5150]

Well organized. Easy to understand PAC Bio's data sequencing technology. Thank you!

[@yanjunhuang4079]

The Polymerase do have the ability to synthesis 1000bp/s, but i have seen many online material said that it is only 3-10bp/s. And it is also very not logical since it will be pretty hard to assemble fragments you described above. So is this really correct or is there technology that control the polymerase reaction rate.

[@evoJohn75]

Yes. The polymerase is slowed down to about 2-3 bp/sec. Much of Pacbio r&d is in modifying polymerase. The description here is a bit off, although otherwise very well done. The whole insert is sequenced each lap around the template, not little bits. There is significant indel errors at single pass accuracy, but they mostly go away with >3 passes

[@ilhambenkharfia6874]

I got nothing what's the relation between pacbio and phospholinked nucleotide and zero mode waveguide ? And thank you

[@sofianaftalynathan1386]

Clear explanation and great presentation! Thank you! :)

[@kareemjeiroudi1964]

Wow this guy has become my favorite professor 😉

[@ewittka100]

Very nicely explained. Thanks.

[@飞檐]

Wonderful explanation, and amazing left-hand mirror writing!

[@minhthuonglethi9874]

Thank you very much for videos.

[@yarasilva6138]

This is increíble. Thank you!

[@MrSeonyeongkim]

very much thank you sir

[@bs7shorts729]

Sir, very informative vedio, hepl me distinguished between illimuna and pacbio rsII

[@SaintNath]

Thank you for the nice explanation you saved me a lot of time with summing this up. I have read about a MinION device using electric current for nucleotide detection. In the video you talked about fluorecence. Could you explain the relation for me or is it simply two different technologies?

[@microbi0296]

It's different technology. It's called Oxford Nanopore. I'm learning about sequencing and came across this article about different technologies. I found it helpful. www.sciencedirect.com/science/article/pii/S0888754315300410

[@BrandonSLockey]

better than my lecturer