UV effects on bacteria time-lapse

Time-lapse footage of bacteria-covered petri plates growing after being exposed to UV light. UV light kills bacteria, with longer exposure times resulting in higher cell mortality.
The elapsed time is shown in each clip. The petri plates were incubated at about 37 degrees Celsius.
The lamp used has two 15 watt bulbs. The lamp has 110 volts and the wavelength is 254 nm, UV-C.

[@mellowm5634]

what if all the bacteria just ran away to the other side?

[@sci-inspi]

E. coli can't move on a solid medium. It would take them a very long time to move to the other side as well.

[@finalbossd]

Sci- Inspi Not to mention that not all strains of E. coli are motile. Perhaps you could have used a nonmotile strain.

[@davidgamingremastered6784]

@@sci-inspi r/woosh

[@chloroplast8611]

@@davidgamingremastered6784 r/notawooosh

[@chloroplast8611]

@@davidgamingremastered6784 r/plzcommitapoptosis

[@sid2339]

What if bacteria apply sunscreen..???

[@sci-inspi]

It should protect them. If the chemicals in the sunscreen aren't bad for them in the first place.

[@Wesley_H]

If the sunscreen is zinc based, they should be fine. If the sunscreen is carbon based, the UV will just heat it up and they’ll either incubate or die trying.

[@andrewglinski4722]

Absolutely brilliant 👏👏👏👏👏👏 Let’s just hope no bacteria’s read this comment or else we’ll have a real problem on our hands.

[@WanderTheNomad]

@@sci-inspi I love how you respond to these questions that were meant to be a joke. Really shows how there are no stupid questions.

[@halloween8711]

'Bacteria' is already plural

[@larissaamores2254]

Why is there so many people talkin 'bout gloves? I work in a microbiology lab on pharma and we do not wear gloves. It' s not like splashes or something. It's all about knowing how to handle them. I believe you did it just fine. Normaly people who's afraid without knolwedge of bacterias get scared.

[@whendeathdeclareswar7458]

Larissa Amores umm! maybe because...its E-coli.

[@UnitSe7en]

@@whendeathdeclareswar7458 Do you know anything about E. coli besides "It makes you very sick"? You are a part of the problem Larissa describes.

[@BlaqZ]

@@whendeathdeclareswar7458 how you shit

[@geomochi4904]

if he gets e coli on his hand he could just wash it off

[@yurisims1798]

Yeah, in school, even when I worked with piranha solution or aqua regia I didn't really wear gloves. The only time we did was for hydrogen fluoride. If I didn't need to wear gloves for aqua regia you probably don't need to wear gloves for E. coli, especially when there's already a shit ton on your skin.

[@bigmofo1122]

You should spread the colonies who survived into a new petri dish and repeat the process until they all survive.

[@sci-inspi]

That's a good way to demonstrate adaption and evolution! Thank you.

[@namlehai2737]

Lol

[@jordanponton255]

Yep

[@greg77389]

That's a good way to create a damn superbug, don't do it!

[@mashamitchell9574]

To create a new super bacteria. No, don't do that.

[@SkiLLsZ]

Hold up bacteria! it is i THANOS

[@bepis2104]

SkiLLsZ u beat me to it,

[@FranseD]

Your profile picture make it better

[@nomnomyourmom]

upvote

[@Asu01]

_"To show you the power of UV light, I covered this specimen in half!"_

[@mango251]

Protect yourself against the might of UV light with Flex Seal!

[@darylcheshire1618]

that is a laboratory UV source, more powerful than the battery UV-C wands.

[@christopherharris696]

@@mango251 that funny, you know Flexseal really liquid Rubber. UV light eats and destroys elastic polymer Rubber. Rubber enemy is UV light. Don't care if it's vulcanizing like tires they degrade. That why sunscreen like a Rubber protection cream lotion. You are actually protecting the dermis, elastin elastic rubbers band's fibers in our skin, from degradation and wrinkles. Our skin, flesh matter or whatever you call it is similar to a Rubber coverage, over our body's. The pigment melanin a natural sunscreen is natural built-in our DNA. But what most don't understand is, the more skin pigmentation we have the more the skin is tighter lest ability to stretch, the less pigmentation and dna of lighter skin types the more elastin rubber like the skin is. That the way DNA ,dermis etc is , but UV will destroy Flexseal🤣😃😄😄, and Rubber and skin.

[@dexterwolford]

How did you get italics?

[@gonelol9495]

I honestly love watching bacteria colonies grow, it’s my favorite thing to watch, it’s interesting to see this though. It reminds me of my science fair project, how much bacteria does make up contain? I had a lot of fun watching the progress of the bacteria colonies and seeing them grow, it was very fun. I would like to try this one day at my schools lab, maybe with something different.

[@katiechaan]

How did you quantify the amount of bacteria left over? Like is there a way to compare the growth of the control side and experimental side? Not sure if that makes sense, but thanks

[@sci-inspi]

Hi Katie, Usually you can count the number of bacterial colonies and compare the different sides. Highly concentrated cultures often leave a "lawn" of bacteria which we can see in the control side. Hope that helps.

[@chucknorris3752]

ImageJ is also typically used so you can measure the area of patches or lawns of bacteria

[@ChristmasEve777]

You can literally see the living bacteria with the naked eye. Couldn't you see the difference between the sides? And he was just counting how many separate groups (colonies) were remaining. Just the number of colonies and total size (area, because they're on a very flat surface) should be all we care about.... not the actual number of bacterium left over.

[@kashewz]

I don't think the OP took basic biology class >.>

[@Imjust-here_]

Afraid you have E. Coli on your skin!? No worries! Lay under a UV lamp for a few minutes. You'll be fine.

[@mr.goldfarmer4883]

love these scientific demonstrations!

[@DavidHelloWorld]

I was hoping a video like this existed. Thank you!

[@vijaypantane]

Thanks the video is a boon to explain community the effect of time and UV radiation on harmful ecoli found in so many water and food items we consume daily.

[@roncom4321]

*Casually pulls out E. Coli*

[@nguyenphamthanhgiang8951]

The experiment is cool enough, but you provide explaination too? Perfect.

[@FightingMario54321]

Anyone else freaking out about him not using gloves while handling the E. coli? Lmao. My microbiology teacher always made sure we handled our Petri dishes with gloves.

[@V0YAG3R]

FightingMario54321 nope

[@0x1EGEN]

This is also why every probiotic drink is in a black bottle, so that UV light doesn't kill the bacteria.

[@SenTHR811]

Isn't it weird how he handles bacteria so normally? No gloves, no equipment, just swab like there's no tomorrow.

[@davidmullerful]

So bacteria are mini vampires actually, themore you know.

[@DenaturedProtein]

Learnt a lot! Keep posting such educational videos स्टूडेंट की दुआ लगेगी😁

[@ahmedask9045]

bacteria: they cant kill all of us. UV Light: Hold my beer im gonna end this shit wholes career

[@TheLostinTheUnknown]

Your content is amazing! Can you do the same experiment with fungi instead of bacteria????

[@sci-inspi]

I'll add it to my list of future videos.

[@TheLostinTheUnknown]

@@sci-inspi Thanks

[@rickslife]

Not mentioned here that the UV light needs to be UV-C light. Not UV-A or UV-B. A or B will not kill bacteria or viruses. That’s a very important note not mentioned.

[@78kurenai]

Wow, thanks for this! I will quit with overnight uv exposure. It was melting plastic tubes and Pipet tips

[@Davis38]

I don't know why but the sentence _"Let's spread E. Coli on the Petri plate"_ Is so funny

[@defaultporing]

Nice video, thanks for sharing. Would moving the light closer to the petri dish kill the bacteria faster? Just wondering...

[@sci-inspi]

I am not sure, but I think it would. I think bringing it closer would make it more intense I assume.

[@Dysfunctionality15]

@@sci-inspi I'm not a biologist so idk if increasing light intensity would decrease disinfection time. But, I do know that uv follows the inverse square law so the light should be far more intense the closer it is.

[@lanhnhusuong8583]

@@Dysfunctionality15 I think it's also depend on UV wavelength, from all I know, 260-270 nm is the best to kill E.Coli. The lower is, the less effective. I'm not sure what happend if UV wavelength is higher.

[@xPowerKittyx]

nail techs worldwide united with their uv lamps

[@end8316]

so this is is the cure to pimples - UV. thank you for this.

[@sci-inspi]

That wouldn't be a good idea. UV light causes sunburn. And prolonged exposure of UV after years can cause skin cancer.

[@drkaranmannan]

UV - C are lethal

[@assassinaria]

It's great for psoriasis though because it kills all the cells that are rapidly regenerating.

[@idk_what_to_sayyes1038]

@@sci-inspi if im correct, it also does give a thing called arc eye or welders eye or something? witch tends to be quite painfull.

[@hardstylecoverage989]

Will it be the same effect with 290nm, UVB ?

[@lisawong8379]

What distance was the lamp from the petri dish? Nice experiment, thank you!!

[@jadsonlucas1456]

the video is 3 years old, but I really liked it, I just missed seeing the PPE protection in your hands when handling the bacteria, or with your eyes in the ultraviolet light. I'm from Brazil and this video will help me do some research

[@ClarkWhite81]

(I liked and suscribed) so if I leave something out in the sun for 2 minutes, will all the bacteria on it die?

[@sci-inspi]

Not all the bacteria, but some will die.

[@Chungustav]

These lights are UV-C which cannot penetrate or atmosphere.

[@saudibert5626]

Is it possible to drink water that has been exposed to ultraviolet rays for several hours, 6 hours, in the sun ? Thanks !

[@denniswong7596]

How much E.Coli would i need for this for this experiment

[@sci-inspi]

one 5-milliliter 24 hour old broth culture of E. coli should be plenty.

[@Keys879]

This is why you dont need to worry about getting sick from outdoor surfaces. Generally speaking. The Sun is a wonderful disinfectant.

[@yakovliam]

The real question is, where on earth did you GET E COLI??

[@sci-inspi]

I bought it from scientific suppliers.

[@jasonharris5739]

its ecoli not ebola lmao

[@hakudoushinumbernine]

So how long would it take a light like that to disinfect someones living room?

[@brodyszone157]

Have u ever been sick from the work u handle with ur hands. I'm cringing so hard my face is gonna get stuck like this lol. But I'm here for this

[@sci-inspi]

Nope, never.

[@samuelmontypython8381]

This would be a great thing to show in vampire movies when scientists try to figure out exactly why vampires are evolving to become immune to UV light

[@transcendentspider1879]

Thanos snaps all bacterias in petri dish dishappears No bacterias were harmed during the making of this video

[@andrewyek]

hii, you have got a good video. would you do a video on Ozon air toward bacteria and mold pls ? there are lot of cheap ceramic ozone generator on ebay.. thanks andrew

[@sci-inspi]

Let me check and see if I can get my hands on some Ozone.

[@andrewyek]

great!! can't wait.. thanks

[@eikosimino5579]

Thank you! Now im gonna spray my piece of bread with UV light so no filth is on it!

[@twentytwo138]

Where do you get your bread from lol.. You don't have to do that if it's fresh bread, bacteria is killed within the oven.. But even if there is some bacteria left, and someone touches the bread, it can build your immunity to that bacteria.. If you disinfect every single food you eat, you wouldn't have immunity and you would easily get sick just by going out

[@Sir_Budginton]

So to protect myself from bacteria I just have to stand under an extremely intense UV light source for extended periods of time. Maybe if UV kills bacteria, it can also kill skin cancer. I think I just earned myself a noble prize.

[@Eltodofull]

@Dušan Xmetov And that's exactly how radiotherapy works! Except it uses x-rays instead of UV radiation. (The former is more energetic)

[@garlicbread7119]

UV kills every cell, high UV exposition is bad for your body.

[@Sir_Budginton]

Garlic Bread that’s kinda the whole joke

[@Arthanax]

@@Sir_Budginton Yeah, since UV is literally the cause of skin cancer. Don't know how garlic didn't get it. :l

[@LorenzoRM85]

is one minute too much for the skin?-how much is too much exposure?

[@chrisscott1547]

Narration, explaining what we're looking at, and avoiding labels on wrong sides would help this a lot.

[@maddogmaz1576]

Why? You wouldn't understand anyway

[@jenny1671]

can anyone tell me what is the best uv wand to get i want to make sure it's not a knock off and it kills the bacteria and i want one i can change the bulb if possible for toilet bed sheets and kitchen counter and my lazyboy. how would a person go about testing if it kills germs as it should.?

[@furrycircuitry2378]

Jeannette K i believe any regular uv light will work but you can always buy some off of amazon good luck:)

[@HungTran-nr6he]

it s not good to use UV light to kill bacteria in closed area like your toilet, because Uv light reacts with Oxigen O2 in the air to form ozone O3, which is toxic!

[@jenny1671]

i recieved a light in the mail with no instruction it is led and it is blue it showed how what surfices needed to be cleaned I think it is a black light. Is there such thing as a led uvc light?

[@adamw.8579]

+Hung Tr Ozone has no smell, this thing we are feeling is nitrous oxide and this is real threat. Because ozone is hyperactive then easy oxidate nitrogen.

[@krashd]

Those with the strongest immune systems grew up playing in the dirt, those with the weakest immune systems grew up wrapped in cotton wool.

[@Deefourteen]

Sunscreen is made to block UV-A and UV-B not UV-C which is what Germicidal UV lamps emit.

[@TypeORedPill]

Does UV light have any effect on viruses, and if so, how much exposure is needed to kill them, especially hard-to-kill viruses like SARS-CoV2?

[@NigelTolley]

Cov-sars-2 isn't "hard to kill", just use soap or any disinfectant. The problem is, it *spreads* really easily.

[@oscararrietav.1311]

@@NigelTolley Billetes, mascarillas, verduras talvez

[@Beelzebububble]

In China, they use cool looking lights (UV) for use in clubs and other entertainment venues. Until patrons complained of bad sunburns.

[@ssszwu]

Is it the same light used for check the money?

[@mu11668B]

Imagine if bacteria says *oof*.

[@peepers4763]

At 4am the music was a bit loud but interesting enough that I watched to the end of the video. Then I wondered is that stock KZbin music?

[@zombieturtleeggboy3572]

If I turned my stereo up really loud and played Barry Manello nonstop and after 1 day later there was no life surrounding the stereo that doesn't mean Barry murdered everybody, they just ran away screaming, covering their ears 'till they were out of earshot. USE TWO PEE-TREE DISHES. ONE WITH, ONE WITHOUT, COVER ONE AND TRY AGAIN.

[@rohitchaoji]

I'm sure you take all the necessary precautions but seeing you handle that petri dish with bare hands while rubbing E-coli on it had me a bit worried.

[@unvaccinatedAndPureBlood]

Nice experiment. So why can't you do this with raw milk? I've always heard there can be some danger drinking it, why not use UV light just to be sure to get a contaminant free drink?

[@AureliusR]

I hope you wore proper eye and skin protection when using UV-C bulbs! Even brief exposure can cause temporary damage, and anything more than a few seconds can cause burns in your eye. Apparently it feels like you have sand in your eyes all the time... some people's eyes seem to recover, others have irreversible damage. All depends on length of exposure and the power of the lamp. Best to just not take any chances. Even standard polycarbonate safety glasses cut pretty much 100% of UV-C light but even though I know it's factually true it still doesn't feel safe to me and I tend to use specialized UV blocking glasses. For those wondering, the reason UVC is so dangerous to humans is that we are never exposed to it naturally. The sun does produce quite a bit of UVC but the atmosphere filters almost all of it out, as well as most UVB. The UVB that gets through is what causes tans/sunburns/melanomas. UVA does also play a part but it's not nearly as damaging as the shorter wavelength UV.

[@RayMak]

That's quite fast

[@anonymouse6129]

6 likes only lmao

[@zia-i]

3:00 Sir, i have a request. How if you smear the bacteria with sunscreen? it would be a great experiment to prove the sunscreen!

[@justward354]

And that will be great idea to check if that sunscreen worth it or not

[@131YOURFRIEND]

how did you gather such bacteria? Your videos are making me loving science. When i was young, my wish was to buy Microscope.

[@sci-inspi]

I have pure bacterial culture in my lab. I purchased them from scientific suppliers.

[@6teeth318]

Cool guitar towards the end.

[@johanponin1360]

Would a simple 3W UV LED be enough ?

[@sci-inspi]

I am not sure. I used a lamp that had two 15 watt bulbs for this video. My guess would be the 3 Watt lamp would require more time to kill the bacteria. If you have access to a microbiology lab, you may be able to test the 3 watt lamp it.

[@TheFlyre]

what if you got a 5 gal of bucket of yeast and water that would take forever?

[@brasildocara]

Sci- Inspi which wavlength in nanometers was the lamp you used?

[@harishsridharan3063]

The wavelength was 254 nanometers

[@sci-inspi]

Yes the wavelength is 254 nanometers. Sorry for the late reply, I never got a notification for this comment.

[@12chachachannel]

0:40 "One half of the petri plate is covered to protect against the UV light. The right half will be the control side." sounds like they are two different sides and is very confusing actually

[@harishsridharan3063]

What temperature did you incubate the bacteria in??

[@sci-inspi]

37 degrees Celsius

[@harishsridharan3063]

Thank you. I was researching this on science buddies and they said 37 degrees Celsius but I wasn't sure and so I asked you what you did.

[@sci-inspi]

You're welcome Harish.

[@benjamintoybonniephillips8018]

bacteriophages: AM I A JOKE TO YOU?

[@शिवणकला]

2:45 High level stuff

[@edwardolkowski4018]

Cool video. But why not just turn the phone over and run the sequence again to get the back?

[@dantemusica]

Hi! Can a single uv lamp be used in a house for clean Corona Virus?

[@helou5904]

You have a soundcloud? What do you upload there, bacteria trap from lil ecoli?

[@sci-inspi]

Lol, E. coli's mixtape is straight fire.

[@helou5904]

@@sci-inspi Gotta listen to it

[@sci-inspi]

Lol it is actually the music I make for these videos.

[@katiedudkowski3937]

So why doesn't the first trial of 15 seconds show anything?

[@fauzanbri]

Plot twist : theres no gloves harmed in this video

[@peterb4926]

distance to the light may also play an important factor in addition to the length of exposure.

[@MukeshKumar-fp4nr]

What happens to Protozones and protozoa

[@nagromgreen]

Now repeat the experiment with cling film over top and apply different spf of sunscreen as well as expired sunscreen to show what level of protection you require for skin protection and if it’s a marketing ploy for expiration dates on sunscreen.

[@That1GGirl]

So if I have a uv light on all day and night in my bathroom there will be no bacteria? 😲

[@angrypastabrewing]

theoretically, yes

[@eoslightpulsesl6224]

Great Video! Whic doses have you applied? 40mJ/cm2 for 30sec? and for 2 minutes? Thanks!

[@sci-inspi]

Hi EOS, I don't know the dose but I could give you information about the conditions and maybe we can calculate it?

[@johnk6324]

What about viruses? Are they affected by UV light?

[@Kayome]

Would be important to be exposed to sunlight for a few minutes with no screen so you can keep bacteria, at least those above the skin, under control? This applies to fungus too?

[@alzack112]

Whatever questions emerged, I'll answer them all as an educator. Thanks, Sci- Inspi

[@sci-inspi]

I try my best

[@bintusayf6921]

Is uv is same with infrared lamp in function? I want to kill the bacteria in my ringworm with using that infrared lamp ( fisioterapi lamp)

[@StrawberryNinjaNibbles]

Why did you use paper to block uv? How can you be sure it was all blocked?

[@sci-inspi]

Uv rays are easliy blocked. You can see in this video I blocked it just with paper.

[@AshGreen359]

For anyone watching this in today's times. UV can also kill viruses. Bacteria are NOT the same as viruses

[@deeps66]

have u tried picking up the ones that grew after exposure, spread them, and re-expose? luv ur channel, btw!

[@mmdirtyworkz]

Come to think of it, why aren't there any toilet cleaners made from UV lamps? Like in pub toilets, you finish and once you are out the UV light blasts and kills any bacteria. Maybe it already exists?

[@Heather_Morgan]

You never mentioned the wattage of the lights. That's obviously a very important factor.

[@sci-inspi]

That’s mentioned in the description.

[@demil3618]

That’s interesting. It shows why spraying streets & pavements in complete nonsense. But I wonder (obviously in context with The Virus): how useful is UV (suppose it’s UV-C, right?) when it comes to disinfecting surfaces (in a shop, train, bus etc)? Does indirect exposure kill? How do I find out the right exposure time? What wattage is needed?

[@flowercollector9013]

This is an awsome channel

[@russell_rozenbaum]

How strong is the UV radiation? Can it be measured on an index like the sunlight’s UV radiation?

[@Fjerid]

I wonder why our ancestors who were in the sun 3 to 12 hours a day, didn’t get skin cancer. And why the cancer incidences skyrocketed when the first sunscreens were introduced to the market.

[@sci-inspi]

They didin't live as long as we did. And people who tan often are the ones most at risk for skin cancer. Sunscreen reduced this risk by blocking UV rays.

[@feynstein1004]

Lol because they were really black i.e. they had a lot of melanin on their skin which converted the UV into harmless heat.

[@wzrdinthemaking]

Thank you for the explanation at the end

[@Disconnected554]

Good Ending: No bacteria survived

[@jakelame7856]

question 1: what happen if animal cell has chloroplast question 2: what happen if you put Archaea and bacteria together question 3: what happen if you put cancer cell and bacteria together question 4: what happen if you put white blood cell and Archaea together question 5: what happen if piece of archea floating in your body

[@sci-inspi]

1. I don't know any animals that have chloroplast, closest would be coral. They have algae inside them that can make energy. But usually eating other things provides a lot more energy than photosynthesis. 2. I don't know. I think they just grow. The bacteria might grow faster. 3. I don't know. I think they just grow. The bacteria might grow faster. 4. I suspect the white blood cells might attack the archea 5. I don't think anything. There are no archea known that are pathogenic. They are found in in the human microbiome as well. Meaning there are some in our bodies already.

[@jeffjularbal9404]

How about uv sanitizing tubes that are used to purify water? The range of the light to the water is point blank. Can you make a content about this? Does uv sanitizing tubes really work?

[@동동동-x9b]

Why do you cover the middle part with the black line that's the most interesting part lmao

[@miguelangeldanielovdikov5007]

Where did you find escherochia coli?

[@sci-inspi]

I bought it from a science company.

[@NikosGardanis]

So the bacteria actually got a bacteria type of cancer?

[@sci-inspi]

The opposite, they couldn't reproduce anymore.

[@Microscopy1]

Very godd footage and good presentation. Subscribed!

[@gfrzcr9982]

Lmao maybe this is why my mother call me bacteria when she was grasping her life on the hospital then died few seconds later

[@jonitan76]

How about corona virus? Is it efective to kill the virus with uv?

[@gaborgabor1338]

What was the distance between the 15W lamp and the petri?

[@sci-inspi]

About 21.5 cm

[@gaborgabor1338]

Thank you! and gratulation for video.

[@mrgreen7754]

Why this video on my recommendation due to choronavirus outbreak? 🤔does youtube recommend us to buy this? 😏😏

[@shijueksdee7090]

Wait wait, whered you get those organisms

[@computerg11k89]

*Perfectly balanced as all things should be*

[@aquareon2347]

my main question is where u got the E-coli from

[@sci-inspi]

I bought it from scientific vendors.

[@deqnq1705]

Either you buy it from some lab or you grow it from your poop because e.coli is most common in the intestines