Shaking Grains for mushroom spawn: Speeding up inoculation times by shaking mushroom grain spawn

Explanation of shaking grains - comparing the progress of various grains to make grain spawn for growing gourmet mushrooms.
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[@BoroBootBoy]

I did the same thing over the past couple of months, as and when i needed to prep more spawn and had a chance to go to the stores , so nice to see it being done in a methodical way.

[@addammadd]

I recently used your king oyster LC on a 50/50 white/brown rice which couldn’t be shaken for obvious reasons. It took about a month and a half at 67f ambient and fully colonized with zero contamination across 2 jars. Not being a commercial grower means my timelines don’t necessitate that extra step. Besides that, I’ve yet to see a side by side comparison of shaken to unshaken grain colonization at scale to see if the time savings is worth the contamination risk (for a home grower who doesn’t have a flow hood).

[@willjames5243]

You grew on me....I'm feeling your vibe now

[@johngailey875]

Id be curious how the brown rice compares

[@Angelina-xj5zd]

Can you over shake? I shook some rye grain spawn about 5 times and it went bad and began to smell sweet strongly so I tossed it. The mycelium stopped growing, I think it died. Can you kill it by shaking or was it contamination?

[@andrerezenderj]

That's a good question. I have the same doubt. Because it seems that when whe shake the jar we are breaking the mycelium and indeed the grains look like starting from zero again. How many times can we do this?

[@johnstamos4629]

This is great, I'm watching em as they come out! Did u cook those grains?

[@FreshfromtheFarmFungi]

yes cooked them and cooled them in front of a flow hood Im making a longer more detailed video just waiting for the results

[@Galvoflysoz]

Hi Gary been following you a couple of weeks now. Was just wondering what the flow hood is that you are using as it looks nice and narrow. I'm think about purchasing one soon.

[@FreshfromtheFarmFungi]

it’s this one from myers mushrooms kzbin.info/www/bejne/r4XTl2Z7gtdkiac

[@stevedog6948]

Can you inoculate lentals?

[@FreshfromtheFarmFungi]

yes they get mushy

[@raya0ivy]

🍄mushlove

[@SIMBIOTICSOUNDS]

what do you think about putting a small amount of verm in the bottom of the jars to soak up excess moisture?

[@FreshfromtheFarmFungi]

it works as well as a few pellets or some saw dust there is some wiggle room for error though but it might be a good buffer

[@joshp5737]

Hey Gary, another great video. Quick question.. i bought one of those GL45 Liquid Media Extractor Caps with the hose going in and the hose going out. Can all that be pressure cooked? or do i need to just bath the smaller parts (like the hoses) in alcohol instead of pressure cooking them before i introduce them to my liquid culture..? Thanks again Gary

[@FreshfromtheFarmFungi]

they can be pressure cooked just wash them out well between uses - after a while the hoses lose their elasticity and need to be replaced

[@joshp5737]

@@FreshfromtheFarmFungi thanks a million Gary

[@granttarget8436]

What's the reason for using small jars like this? I see you use them alot. Are they Vintage pint jars?

[@FreshfromtheFarmFungi]

less space means more variety that’s the main reason and they fit on the shelf better

[@granttarget8436]

@@FreshfromtheFarmFungi thanks for he reply. what do you do with them? make more spawn?

[@Seth-ff5lp]

Not too knowledgeable on the mycology, but I'm wondering can I make a liquid culture of any mycelium? Seven if it doesn't have a fruiting body?Like soil dwelling fungi Glomus intraradices ?

[@FreshfromtheFarmFungi]

possibly - some species are more difficult than others

[@johnriperti3127]

Is there a reason you use small jars ? or is it just for demonstration ?

[@FreshfromtheFarmFungi]

I use smaller jars for breeding each jar goes to one bulk bag so I can breed lots of phenos in the same round - for production I scale up

[@ibrahimozer7955]

Hi Garry. I need a help if you know the answer you will make me so happy I wanna cultivate a truffle mushroom. Which plate should I use PDA,MEA,SDA or anything else on the other hand if you have any idea can give me advice thank you very much. I am so appreciate

[@FreshfromtheFarmFungi]

I think it’s best to start with spores and inoculate the roots directly but for agar Im not sure maybe try a few and see what’s best 👍

[@foxwonderland8467]

How much liquid culture did you put in each jar?

[@FreshfromtheFarmFungi]

2 cc

[@user-do7lp9fl8i]

👍🍄🍄

[@AnnnSweeps]

why not just shake them after injecting LC?

[@FreshfromtheFarmFungi]

that works too