Excellent videos! Short and sweet with appropriate visuals to help understanding. Please do more of these! :D
@DaruoshAghajaney4 жыл бұрын
What a brilliant lecture. Thank you.
@olli14923 жыл бұрын
Very good Job explaining! This should be higher up in the search list.
@joelevi98233 жыл бұрын
Again..your videos are just amazingly explained so clearly
@Microcourses3 жыл бұрын
Thank you so much 😀
@williamflanagan80033 жыл бұрын
Another top quality vid.
@damiancavazos70439 ай бұрын
Thank you for your videos
@taoliu63344 жыл бұрын
The curve at 1:30 under noise was used in today's online workshop over Zoom!
@Microcourses4 жыл бұрын
Good eye Tao! Talley made those slides, and we all use them. :)
@shivalidongre41234 жыл бұрын
This was great! Could you please post a video on Fourier transform?
@Microcourses4 жыл бұрын
Thanks! It's on the list of future videos. In the meantime, Bo Huang's video in the iBiology microscopy lecture series is very good.
@shivalidongre41234 жыл бұрын
@@Microcourses thank you! I will check it out
@davidkoleckar43373 жыл бұрын
awesome, thank you :]
@Walaa9184 жыл бұрын
Thank you
@kunlin5794 жыл бұрын
say you're using a 60X objective with a wide field, would binning decrease the resolution? Pixel size w/o binning is probably going to be in the neighborhood of 125nm or so depending on the configuration, and binned pixels will be at least double that, what do you recommend?
@Microcourses4 жыл бұрын
Optimal pixel size depends on the numerical aperture of the objective lens (in addition to magnification - there is a microcourse on numerical aperture if you aren't familiar with it) and what you are trying to image (ie, diffraction-limited objects would require a different pixel size than whole cells), so there isn't a standard recommendation. I recommend you post the question on our microscopy discussion forum at forum.microlist.org - it's quite active so you should get some answers there!
@dummag412611 ай бұрын
increase the subject illumination!!!
@Microcourses11 ай бұрын
Sure, with the trade-off of increased photobleaching, and phototoxicity in live samples.