Couldnt ask for a more clearer explanation than this, you made my day. Thank you so much!!!
@fliplipify5 жыл бұрын
One of the best videos about PSF out there!
@maxkotlarchyk42422 жыл бұрын
I seriously applaud these videos. They are so thoughtfully put together and present the information in the clearest way possible. I can only hope that every instructional video out there some day explains things in an equally engaging, informative, and simple format. Well done!
@Microcourses2 жыл бұрын
Wow, thank you! I'm so glad you find them helpful.
@tomkache38575 жыл бұрын
Fantastic video. The visuals are very well made! Also appreciated the image of the Abbe memorial. I see it almost every day when I walk to uni. :D
@tinglianyuan30989 ай бұрын
Lovely to see the pink heart picture flashing out when doing the axial scanning (around 3'30") and she says "the PSF is beautiful!".
@AleeKillingTime6 күн бұрын
Very well put. Thanks for this video.
@turugasairam28863 жыл бұрын
The best explanation on PSF. Completely agree with all the comments below. Thank you very much
@evanoberster47662 жыл бұрын
This is by far one of the bests videos I have ever seen.
@yizhou8665 жыл бұрын
One the best lectures of PSF
@karankhajuria24374 жыл бұрын
Rightly said an expert can make it simple!! Muchas gracias!
@richardred43969 ай бұрын
you were able to make me understand these concepts with a 12 minute video
@dsasmalmnp11 ай бұрын
Fantastic.. I just taught a PG class on PSF.. This helped me a lot to prepare materials.
@amribrahim78503 жыл бұрын
amazing video. I can't wait for future courses
@kochipj Жыл бұрын
Pretty neat explanation! What is missing for a nerd like me are some remarks on the differences between coherent and incoherent imaging and Abbe theory of image formation. What was really enlightening to me was when I read the treatment of Goodman on Fourier Optics that shows that in the coherent case, Abbe theory based on the collection of diffraction orders from the object interfering in the image plane and the convolution approach using the Amplitude PSF are equivalent, because the amplitude transfer function involves a second fourier transform of the Airy Disc PSF that gives a circ while the incoherent case (e.g. from a self-luminous object) yields the autocorrelation of the aperture function because of the square operation in the spatial frequency domain (convolution theorem).
@pamulyadav42752 жыл бұрын
Besides the visual stress felt from staring at the bright circular light shown in the initial half of the video, it was an amazing explanation on point spread function.
@zedius242 жыл бұрын
Best explantion out there. Congratulations.
@omarnofal40223 жыл бұрын
Thank you so much for this informative video!
@hamidsediqi64753 жыл бұрын
Thank you so much for this video! Really helped me understand PSF without having to read pages and pages of writing!! You deserve more subscribers.
@Microcourses3 жыл бұрын
Glad it was helpful!
@sumonsumon44743 жыл бұрын
Excellent illustrations of PSF
@ajdharmasiri48212 жыл бұрын
wonderfully explained! Great narrating too!
@angelbarra57943 жыл бұрын
this is an amazing explanation about PSF. Thank you very much!
@dailytrips2654 жыл бұрын
Very clear explanation, thanks.
@tymothylim65503 жыл бұрын
Thank you very much for this video! It was very informative and I learnt how the PSF relates to the Rayleigh resolution limit!
@user-os3gw7ds4h2 жыл бұрын
Thank you for your great explanation!
@akritino74373 жыл бұрын
Now I get the whole point of interference and diffraction in microscope!
@Mixeygoat3 жыл бұрын
Incredible video! Really helped me out with my microscopy class
@Microcourses3 жыл бұрын
Great to hear!
@drmksu382 жыл бұрын
no body can explain like this thank you very much
@PunmasterSTP4 ай бұрын
PSF? More like "Perfect videos; you're the best!"
@niceday20153 жыл бұрын
Thank you for the brilliant lecture
@Microcourses3 жыл бұрын
Glad you liked it!
@Yagyaansh3 жыл бұрын
excellent video, damn!
@superdogmeatmeat2 жыл бұрын
amazing video
@surya-td4dg2 жыл бұрын
thank you for this amazing content :)
@manishsuthar4002 Жыл бұрын
I've never come across lateral and axial in these waves... Thanks...
@pendalink3 жыл бұрын
Nice video. However, two slits in front of a classical wavefront demonstrating interference is not the double-slit experiment; that refers specifically to the self-interference of a single particle's encountering a double-slit.
@poodook4 жыл бұрын
So the PSF is essentially the impulse response of the imaging system?
@aatt32093 жыл бұрын
excellent!
@laraachaji75842 жыл бұрын
Thank you ♡
@alessiospaziani26954 жыл бұрын
really useful
@AP-vd8ve3 жыл бұрын
Hello. I found your video very explanatory, since I'm currently doing a presentation about PSF and diffraction limit for my University. Can I use some of your visuals for the presentation, and if so how can I give reference? PS sorry for poor english
@Microcourses3 жыл бұрын
Yes, please do! You can reference the channel URL: KZbin.com/microcourses.
@AP-vd8ve3 жыл бұрын
@@Microcourses although I finished the presentation , thank you, for giving me the permission and for helping me understand the terms
@diatomsaus2 жыл бұрын
This is an exceptional video, but the diatom at 1:00 is certainly not Pleurosigma angulatum. Not trying to nit-pick or act smug, this is one of the best PSF videos I have watched so far.
@Microcourses2 жыл бұрын
Thank you! My records may be incorrect. What species is it?
@diatomsaus2 жыл бұрын
@@Microcourses I think it should be Frustulia rhomboides.
@akritino74373 жыл бұрын
Just a question. What wavelength is being spoken about here? It is the emission wavelength of the green light right?
@Microcourses3 жыл бұрын
Yes, emission wavelength.
@sophieastrof4 жыл бұрын
how does enhanced resolution work (e.g. closing a pinhole a little bit)?
@CubanSauce2 жыл бұрын
Well I’ll just watch this 20 more times until my dumb brain catches on.
@amir.rahmani4 жыл бұрын
Are all fluorescence emissions coherence to make an interference pattern at the image plane?
@Microcourses4 жыл бұрын
Fluorescence emitted from a diffraction limited spot is partially coherent, and will therefore result in an interference pattern.
@zhenhongdu4 жыл бұрын
Thanks for your great vedio about PSF,do you have another vedio about OTF?
@Microcourses4 жыл бұрын
Not yet, but it's on our (long!) to do list.
@jacobvandijk65252 жыл бұрын
By the way, it is one MAXIMUM and two or more MAXIMA ;-) @ 3:53 And this really is 2x the amplitude ;-(
@mistericeberggg36775 жыл бұрын
Stop the vocal fry please
@mar2lka3 жыл бұрын
I am very curious if you ever commented that under a video of a male speaker...