The Point Spread Function

[@rahulmangalampalli4846]

Couldnt ask for a more clearer explanation than this, you made my day. Thank you so much!!!

[@fliplipify]

One of the best videos about PSF out there!

[@maxkotlarchyk4242]

I seriously applaud these videos. They are so thoughtfully put together and present the information in the clearest way possible. I can only hope that every instructional video out there some day explains things in an equally engaging, informative, and simple format. Well done!

[@Microcourses]

Wow, thank you! I'm so glad you find them helpful.

[@tomkache3857]

Fantastic video. The visuals are very well made! Also appreciated the image of the Abbe memorial. I see it almost every day when I walk to uni. :D

[@tinglianyuan3098]

Lovely to see the pink heart picture flashing out when doing the axial scanning (around 3'30") and she says "the PSF is beautiful!".

[@AleeKillingTime]

Very well put. Thanks for this video.

[@turugasairam2886]

The best explanation on PSF. Completely agree with all the comments below. Thank you very much

[@evanoberster4766]

This is by far one of the bests videos I have ever seen.

[@yizhou866]

One the best lectures of PSF

[@karankhajuria2437]

Rightly said an expert can make it simple!! Muchas gracias!

[@richardred4396]

you were able to make me understand these concepts with a 12 minute video

[@dsasmalmnp]

Fantastic.. I just taught a PG class on PSF.. This helped me a lot to prepare materials.

[@amribrahim7850]

amazing video. I can't wait for future courses

[@kochipj]

Pretty neat explanation! What is missing for a nerd like me are some remarks on the differences between coherent and incoherent imaging and Abbe theory of image formation. What was really enlightening to me was when I read the treatment of Goodman on Fourier Optics that shows that in the coherent case, Abbe theory based on the collection of diffraction orders from the object interfering in the image plane and the convolution approach using the Amplitude PSF are equivalent, because the amplitude transfer function involves a second fourier transform of the Airy Disc PSF that gives a circ while the incoherent case (e.g. from a self-luminous object) yields the autocorrelation of the aperture function because of the square operation in the spatial frequency domain (convolution theorem).

[@pamulyadav4275]

Besides the visual stress felt from staring at the bright circular light shown in the initial half of the video, it was an amazing explanation on point spread function.

[@zedius24]

Best explantion out there. Congratulations.

[@omarnofal4022]

Thank you so much for this informative video!

[@hamidsediqi6475]

Thank you so much for this video! Really helped me understand PSF without having to read pages and pages of writing!! You deserve more subscribers.

[@Microcourses]

Glad it was helpful!

[@sumonsumon4474]

Excellent illustrations of PSF

[@ajdharmasiri4821]

wonderfully explained! Great narrating too!

[@angelbarra5794]

this is an amazing explanation about PSF. Thank you very much!

[@dailytrips265]

Very clear explanation, thanks.

[@tymothylim6550]

Thank you very much for this video! It was very informative and I learnt how the PSF relates to the Rayleigh resolution limit!

[@user-os3gw7ds4h]

Thank you for your great explanation!

[@akritino7437]

Now I get the whole point of interference and diffraction in microscope!

[@Mixeygoat]

Incredible video! Really helped me out with my microscopy class

[@Microcourses]

Great to hear!

[@drmksu38]

no body can explain like this thank you very much

[@PunmasterSTP]

PSF? More like "Perfect videos; you're the best!"

[@niceday2015]

Thank you for the brilliant lecture

[@Microcourses]

Glad you liked it!

[@Yagyaansh]

excellent video, damn!

[@superdogmeatmeat]

amazing video

[@surya-td4dg]

thank you for this amazing content :)

[@manishsuthar4002]

I've never come across lateral and axial in these waves... Thanks...

[@pendalink]

Nice video. However, two slits in front of a classical wavefront demonstrating interference is not the double-slit experiment; that refers specifically to the self-interference of a single particle's encountering a double-slit.

[@poodook]

So the PSF is essentially the impulse response of the imaging system?

[@aatt3209]

excellent!

[@laraachaji7584]

Thank you ♡

[@alessiospaziani2695]

really useful

[@AP-vd8ve]

Hello. I found your video very explanatory, since I'm currently doing a presentation about PSF and diffraction limit for my University. Can I use some of your visuals for the presentation, and if so how can I give reference? PS sorry for poor english

[@Microcourses]

Yes, please do! You can reference the channel URL: KZbin.com/microcourses.

[@AP-vd8ve]

@@Microcourses although I finished the presentation , thank you, for giving me the permission and for helping me understand the terms

[@diatomsaus]

This is an exceptional video, but the diatom at 1:00 is certainly not Pleurosigma angulatum. Not trying to nit-pick or act smug, this is one of the best PSF videos I have watched so far.

[@Microcourses]

Thank you! My records may be incorrect. What species is it?

[@diatomsaus]

@@Microcourses I think it should be Frustulia rhomboides.

[@akritino7437]

Just a question. What wavelength is being spoken about here? It is the emission wavelength of the green light right?

[@Microcourses]

Yes, emission wavelength.

[@sophieastrof]

how does enhanced resolution work (e.g. closing a pinhole a little bit)?

[@CubanSauce]

Well I’ll just watch this 20 more times until my dumb brain catches on.

[@amir.rahmani]

Are all fluorescence emissions coherence to make an interference pattern at the image plane?

[@Microcourses]

Fluorescence emitted from a diffraction limited spot is partially coherent, and will therefore result in an interference pattern.

[@zhenhongdu]

Thanks for your great vedio about PSF,do you have another vedio about OTF?

[@Microcourses]

Not yet, but it's on our (long!) to do list.

[@jacobvandijk6525]

By the way, it is one MAXIMUM and two or more MAXIMA ;-) @ 3:53 And this really is 2x the amplitude ;-(

[@mistericeberggg3677]

Stop the vocal fry please

[@mar2lka]

I am very curious if you ever commented that under a video of a male speaker...