Single Syringe Method of PRP Preparation

This video is about how to make PRP without a commercial kit

[@huongphan5059]

oh! The same my technique ( Dr. binh duc le , Quang ngai, viet nam )

[@msmarytran4653]

Thank you 🙏

[@aworkerthatcared]

thank you for these videos. You mentioned in the videos that after preparing the plasma, you could cool it and administer it, or activate it and administer it. Does that mean that you do not necesarily have to activate it? thank you!

[@rejuvacare8925]

Whether you activate it or not will depend on your application. The platelets will be somewhat activated on injection by collagen in the tissue. If you want more activation in a localized area (tendon injections) then you would probably want to activate it before injection so that a fibrin clot will rapidly form and keep the platelets localized. If you want the PRP to spread and slowly activate (some cosmetic applications) then you would not want to activate it before injection.

[@sharadjainjbp]

YOUR EXCELLENT PROCEDUTE.What is a procedure you use for IPRF?

[@tedharrison3356]

We have studied IPRF using the Arthrex ACT system, but we don't generally use it.

[@yafesdoski9888]

Thank you for nice study..and where can we fine the app "PRP calc"?

[@tedharrison3356]

PRPcalc is available in the Google Play store.

[@thenomadicDR]

Don't you need to spin the 2nd syringe as well?

[@tedharrison3356]

The second syringe already contains concentrated PRP. There is no reason to spin it again. However, if you wanted even more concentrated PRP you could spin it again and then separate the layers once more. But of course if you do this you will get a reduced yield because of platelet loss during the process.

[@sairaclinic1366]

Same method of prp formation suitable for cosmetic purposes as well?

[@tedharrison3356]

Yes. This method produces standard PRP, good for all uses.

[@victorgalvez927]

and how do you activate it? I mean once activated by CaCl2 yo wait until the clot is formed or you inject prior to the clot formation?

[@tedharrison3356]

We generally activate by adding CaCl2 immediately before injection. We inject before it clots.

[@ales7206]

Which concentration of NaCi is optimal for the syringe?

[@prithvirajvhegde6722]

Save as as Important

[@TH-qv6ef]

Why do you add the red blood cells?

[@tedharrison3356]

I think you may have misunderstood. We don't "add" any RBCs. We include the part of the buffy coat that is stained with RBCs because about half of the platelets are in that portion. Yes, this means we have some RBCs in the PRP. But without that portion our platelet yield would be much lower.

[@TH-qv6ef]

@@tedharrison3356 I never knew this. Thank you!!

[@Dr-789]

How to activate it ??

[@rejuvacare8925]

Most people use calcium. Either calcium gluconate or calcium chloride will work. We use 10%CaCl3 at a 1:20 ratio. You can also use thrombin or a host of other chemicals (platelets are very sensitive!) but we have no experience with these.

[@Dr-789]

@@rejuvacare8925 ....it means u simply add cacl with PRP in ration of 4:1...e.g 4 ml of PRP with 1 ml of cacl and then mix PRP with cacl by shaking ?

[@rejuvacare8925]

@@Dr-789 No, the ratio is 1:20 as I said above. E.g. 0.2ml of CaCl3 in 4ml of PRP.

[@prithvirajvhegde6722]

Single syringe PRP