Next generation sequencing

[@ronniebo635]

I agree with the first comments, I don’t think Ion Torrent works this way. But this is the best, thorough, and most importantly, succinct way I have seen NGS presented. Your visuals are great! Keep it up!!!

[@alaaadelmohammedahmedskran4430]

U R a great instructor thanks from Egypt ^_^

[@-by7659]

You r my best Biology teacher

[@shomusbiologyofficial]

Thank you so much for appreciating my efforts

[@aditalammim5927]

Somu, love u. U r a genius. Brilliant work bro.

[@shomusbiologyofficial]

Thank you so much for appreciating my efforts

[@KalaiSelvi-he4xz]

Really helpful sir🤝👍👍👍

[@shomusbiologyofficial]

You're welcome

[@aamrollahi]

This was very clear an helpful. Thanks

[@mayukhroy5393]

This video is helpful Sir.

[@kunjukunju4672]

Sir, Can you please do A video about SOLiD sequencing ,

[@priya18won]

VERY WELL EXPLAINE, THANK U

[@shomusbiologyofficial]

You're welcome

[@anacaro5503]

Great video, good explanation. Thanks

[@bilalasif7036]

Ana Caro kkk

[@yingliweng9759]

very helpful, thanks

[@shomusbiologyofficial]

You're welcome

[@bszoologest3303]

what is the difference between pyrosequencing and 454 sequencing

[@aryaram8192]

Thank you Sir

[@shomusbiologyofficial]

You're welcome

[@areejnbari1940]

you have Patreon? bcz you are SO HELPFUL !

[@shomusbiologyofficial]

Actually I don't have patreon but I want to make the account soon

[@areejnbari1940]

@@shomusbiologyofficial THAT WOULD BE AMAZING. CANT WAIT.

[@krizzy4]

Hi Shonu...good presentation but do U think Ion Torrent works that way...it is semi conductor tech and not optics and florescence...correct me if I'm wrong..I think U are talking about SGS from Illumina...

[@manishawalunj5976]

+Christo Rajan yes, I too feel same thing. according to my knowledge Ion torrent works in different way. H+ ion which is releasing after addition of each dNTPs.

[@Khafizofff]

Shomu is wrong

[@sivakumarmalka]

Excellent.

[@shomusbiologyofficial]

Thank you so much for appreciating my efforts

[@sivakumarmalka]

@@shomusbiologyofficial Shomu Ji, you are an excellent teacher.

[@mysticalgirl19]

very informative video, thank you :) How would you use NGS to identify Amyotrophic lateral sclerosis (ALS) with focus on RNA binding proteins TDP43, FUS, TAF15? What would be the approach?

[@rahafh8555]

Thnx 💜💜💜💜💜

[@bubaihazra1083]

Master please upload on video on summary of topic ' molecular biology' with short description, uses, advantage and disadvantages of these methods.... And thank you very much for this novel duty

[@ManashRaja]

Thankyou Shomu Da

[@adityasaha2719]

Sir if the sequence is unknown then how can we design a primer specific at first??

[@rude_pahadi6423]

Primers will be designed against the adapter sequences which we have ligated to the DNA fragment

[@anjanamondal6224]

Thanku so much

[@shomusbiologyofficial]

You're welcome

[@sagarrokade49]

Sir if we fragments the DNA then how we get the full DNA sequence?

[@mansithakkar7488]

Overlapping sequence helps to detect the full DNA sequence.

[@kikelomoolatunde120]

Please can you make a video on massively parallel sequencing

[@shomusbiologyofficial]

Okay

[@arindampaul5256]

Sir how to design primer for next generation sequencing?

[@docgarg1596]

Can u pls explain oligonucleotides in detail in a new video

[@DaFan86]

Thank you!

[@shararehmahmoudian355]

Wow...tnx!

[@shomusbiologyofficial]

You're welcome

[@CrazyGamer-xi8rf]

"-and I hope that's helpful." Copyright Bozeman

[@MrSubham68]

how many types of NGS are there ?

[@AwaisAkram7777]

I have a confusion "What's different between 2nd Generation sequencing and next Generation sequencing ??"

[@a200120021]

Thx!!

[@Theprofessionalsurgeon]

im sad cause i didnt see ur face in this vid

[@agrpayal]

you sound like rajesh of big bang

[@MegaMie77]

...and you look like Priya.

[@HaiNguyen-db1ot]

What is barcode? Thanks

[@febyermina3853]

Barcode is kind of DNA markers, it makes us know the target DNA to be sequenced . Barcode can be flourescent dye or radioisotope. Hope it helps you to understand

[@zachp2120]

The barcode can also be used as an identifier for the patient this way multiple patient samples can be sequenced at the same time with no confusion on which is which

[@chainikagupta5360]

why it is called ion torrent?

[@cellbioinfo]

Hydrogen ions are released during the polymerization of DNA are detected and the Graph Generated on the basis of such Nucleotide there for it is called ion torrent sequencing....

[@bszoologest3303]

explain me sir please

[@kunjukunju4672]

Sir , Can you please do a video About SOLiD Sequencing

[@shomusbiologyofficial]

Okay

[@kunjukunju4672]

@@shomusbiologyofficial Hope you do the video soon

[@AwaisAkram7777]

I have a confusion "What's different between 2nd Generation sequencing and next Generation sequencing ??"