Western blotting technique | principle and step by step procedure

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Sir, do you provide any notes

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But I can't find autoradiography topic

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[@rogersevers123]

Shomu this is an excellent tutorial on western blotting. I just read the methods section of a research paper on western blotting and didn't understand much but after watching ur video I can confidently say I understood everything in that method section of the journal now! Thank u!

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[@aliakhan-fn3bm]

Sir thank you so much for western blotting explanation. I have exam on 17oct n it will help me.meri dua h k Ap aise hi sabki help Karein or Apka shomu biology khub tarakki Kare Inshallah 🤗😊

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Because of you sir my concepts are very clear about the topics that I listened ur lectures.. you are really an awsm teacher..May Allah bless you with more Knowledge And Success..🙂

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excellent sir...I am verymuch impressed by the way you teach

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Thank you. Whenever I am curious about something, your videos help me with my curiosities clearly.

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God bless you Sir, I have an exam in two weeks, and this information was really helpful🙏🙏

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A very didactic and informative tutorials. You are doing great!

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Shomu you too Sabi book. You explain everything so succinctly. Well done.

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No one can explain with such a beutiful way u explain. Thanku very much

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Sir your teaching style is awesome...l understand everything which you used to deliver.

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After watching it i m excited to perform it in lab. Thank u sir u r the best teacher

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Shomu, our biology professor asked us to take notes on this video for homework! you rock!!:))

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This week we executed this and it is remarkable.

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It is remarkable in deed

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i have an exam tommorow and this really helped thank you 🙏🏼

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[@Naveenkumar-wt6qp]

excellent, being a chemist, still I could understand very nicely. Thanks for your clear explanation

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[@maryamrahim342]

Yesterday i watch ur video about southern bloting nd today i help my frndz .they easily understand the topic.

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big round of aplause for that comprehensive explanation

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You are legend, Sir. what an explanation.....superb...

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you are really good deliver the lectures Alhumdullilah my all blotting are cleared now ...Thank u soo much

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The best explanation of western blotting ! thank you

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Explained far better than my professor. Thank you 👍

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That was a very simple and perfect explanation..Thank you very much ☺

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This is the best video I have found on this topic, thank you so much!!!!🤗

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Kitna samjhne ki koshish ki maine par nahi hua par tum to kamal nikle yaar....😎 Owsome ❤️

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Your explanation was great.

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great job👍 it is the simpliest way to remember the procedure. thanks

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Your work is highly understandable I appreciate it thanks

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Thanks alot sir , my exam is on 14th of May and u helped me alot in this topic ...anyways you help me everytime..hehe ..you are a genius sir..may god bless you ...❤️

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Ur explaining very well kept it up . Lots of love

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Thank god to give me a chance to watch this video, it does help me a lot!

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really to good for the beginner as well as experienced graduate and postgraduate

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thanks for video , i have exam today , if this comes in exam i will bless you by heart still thank you

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Thank u sir for urs traching while comparing my lecturer u teaching is super

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K sir

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Best teacher ever!

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Hello, Apka bohot bohot shukriya Jo ap etne mhnat se sabkeliye ye information as in the form of lect. You're presented. Pata nahi apne sabko achese deep level Tak samjh aaye common language mai sab chizo ka dhan rakha, I'm from Maharashtra, Mujhe Bengali to Ati nahi hai wese, Par ye apke liye,,, #AtiSundarama 😊😇😇😇

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Thank u sir i had written what u said in all blotting topics. Thank you very much for for the knowledge u given to me

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Super raa...😂😂

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My interest in botany is only because of you sir thank you so much

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Informative.. Good explanation.. Thank you

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Sir ur explanation is really awsm... u r my real hero... ty sir

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Excellent sir and superb method of ur teaching

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You are the best teacher!

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This is so helpful! The concept behind every step is explained clearly, brilliant video!

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+Riya Paul thank you. I am so glad to hear that

[@Lawrrenz]

SDS-PAGE DOES NOT separate molecules by charge, only by polypeptide chain length (size) 5:04

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Oh Sir another concept clearing video❤

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thank you for explaining how you did!

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More much good explanation sir.

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Shomus, Great collection of videos, one thing to let u know about please, the sound of taking lids off and on your markers is really annoying.

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Good Bless u sirr..Ur teaching method is so Good❤

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Great informative explanation, greets from Egypt bro.

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the best explanation so far. wish i could give multiple thumps up.

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Sir thank you so much for this ... this helped me learn this topic for my final exam.

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I can't ask for better explanation... Thank you man 👏

[@siddheshpundekar6492]

thanks to you sir... I'm preparing for my exam from ur lectures.. tomorrow is my exam. already studied rflp rapd aflp southern and Northern blotting.

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+siddhesh pundekar all the very best for the exam. Hope you will do good

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Thank You very much Suman Dada for all those helpful videos.. God bless you and your success reach the sky..

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tomorrow is my exam... this is helping me alot... thankyou so much sir

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Tqqq sm bro ... doing really great work. ...👍👍 God bless you..❤️

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respect to you the biotechnologist genious

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U r such a great preacher...man...hats off to u👍keep d pace

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Thanku Thanks alot.. it is very useful for me n helpful in my bachelor's ppr..

[@shomusbiologyofficial]

+huma qidwai glad to know that

[@aritribhattacharyya]

I have a few questions as stated below: 1) why did we attach the negative electrode to the SDS-gel if it already contains a negative component i.e. the sulphate that helped to denature the protein? 2) If multiple Ab binds to a specific protein,is there a chance to get improper result? 3) When did we add the Luminol?

[@kayceedey4u679]

A key part of the lecture he missed is the role of SDS and that West blotting detects Amino acid (AA) sequence for proteins (other blots detect nucleic acid sequence). AA have a Verity of charges. On treating the denatured protein (I.e AAs) with SDS, the SDS coats the surface of the unfolded AAs to give the protein a net negative charge. Hence the proteins have a -ve charge and move towards the positive electrode attached to the membrane. You need both -ve and +be fields to run electrophoresis. 2. Antibodies are usually highly specific to antigens, but in this case,some stray away and bind to the membrane (Hence "blocking" is necessary or you get false +ve results) 3. The secondary antibody which binds to the primary antibody (an anti-immunoglobulin) is conjugated with an enzyme eg Horse radish peroxidase (HRP). HRP catalyzes the degradation of the substrate luminol. As Luminol degrades, it emits light the position of which is detectable on x-ray film

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Wonderful explanation Sir.

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you are really an excellent teacher .. feeling grateful to find your page

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Respect for U.

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Sir thankyou so much...well explained...

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[@avisheksarkar1328]

supper....students of life sciences will be gratefull to u....love and respest to you...

[@mulugetagmedhin3787]

You give me a nice explanation. Thank you. Mulugeta from yekatit 12 hospital medical college ( Ethiopia ' Africa)

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[@KaramBiotech]

Shomu , you are really a good guy and thanks for your videos , i am subscribing you from Iraq .. I have only very simple note about this video .. dear brother when you draw the membrane after blotting , the transferred bands should be resemble to bands you did draw on gel after electropheresis , bcz this step only for transferring , but at the last step ( probing ) this will not happen bcz only target protein will appear . Many thanks

[@shomusbiologyofficial]

+Karam Dawood yes. I know. That is the thing shown in the video

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sir you're the best. thanks a lot and Well done

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[@dr.praveentripathi7931]

Wonderfully explained.

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