Northern blotting technique

[@nidhinmangalasseri4061]

Im a biotechnology student......if i got a good mark in the exam its only because of you..... thanks.... All the best

[@mercypeach9919]

I am a biotechnology student too, but I love his explanations I really understand. I am in Uganda but I want to work from USA how can I get a chance?

[@iflafayaz576]

Thank you 😘having exam in biotechnology on 22🙏😘

[@cindrellaray7197]

A teacher like you is a blessing of God. The way you break down such complex theories which are so boring to read from books and manuals into this simple way it feels like a story telling. Thank you Sir for this genuine effort. Your dedication and excellent way of teaching has changed the lives of millions of students for the better.

[@shomusbiologyofficial]

Thank you so much for appreciating my efforts. Glad to hear that you're getting benefit from my lectures. Please stay tuned for more

[@FootyShorts510]

This guy is the goat. Very informative and easily digestible material, keep up the great videos!

[@shomusbiologyofficial]

Thank you so much for appreciating my efforts

[@swatisucharita7557]

Best Bio teacher ever in my life. thank you sir😇

[@shomusbiologyofficial]

Thank you so much for appreciating my efforts

[@Emma-dm2cd]

Hi Shomu! I've been watching your videos for a few years now, and never really took the time to express my gratitude. Every video furnishes a detailled and clear explanation, with a calm and slow voice. I really enjoy it every time, and always am less ignorant when leaving your channel. Thanks again, I wish you all the best.

[@143krishnalearning]

&जीजी

[@Nayl.Photography]

i did not understand even by watching 3d animation of the technique but by watching this video now i understood the whole process thankyou for existing

[@shomusbiologyofficial]

You're welcome. Glad to hear that you're getting benefit from my lectures

[@lexfilyk4704]

this was actually way better than the way my prof explained it.

[@shomusbiologyofficial]

Glad to hear that you are getting benefit from the videos

[@falgunigurjar5479]

For 4 years of my btech journey i paid to university but got to understand topics only because of sir. Each and every topic of biotechnology is explained in such a detail that we just need to see his video and ur prepration is done. One of the best teacher 💯

[@shomusbiologyofficial]

I am glad to help you out

[@yashashreejoshi1050]

Sir I'm a bioengineering student nd we ve genetic engineering molecular biology nd genetics all these subjects i have studied through your coaching sir nd had got vevry good marks thank u so much sir God bless u a lot u are a true passionate teacher

[@shomusbiologyofficial]

Thank you so much for appreciating my efforts

[@tig_ress]

yaaaaaaaaaar someone tell this guy i fell in love with him

[@timotejjadud494]

absolutely fantastic..Southern blotting or Northern blotting, all clear now. Very gifted man, wish you all the best!

[@lyanadhamirah7381]

thank you so much sir, for deciding to become a youtube tutor. all your videos help me so much!!

[@shomusbiologyofficial]

Glad to hear that you are getting benefit from the videos

[@dikshagaunkerd.5973]

Sir, I am having my presentation on Blotting techniques. After watching this video (northern blotting, southern blotting and weatern blotting techniques) i am very confident that i will defenatly do better and get good marks. Thank you.

[@shomusbiologyofficial]

+Gavker Dixa thank you. It's nice to hear that you are getting benefit from the videos

[@Kysersozé1]

You are absolutely brilliant. Thank you for your help.

[@shomusbiologyofficial]

You're welcome. Glad to hear that you're getting benefit from my lectures

[@KsLc107]

Hyy,... I am Be-tech Biotechnology student of 2nd year..... Jst want to tell you that u became a inspiration to all the biology students and also to the Teachers of science section across the world..... thank u. so.. much for helping us.... and not going frustrated during this lockdown... Teachers has started sending the notes and also they will be conducting the test next week......... without any explaination to students they jst provided a book pdf which will not help the students....... and here u came as a Saviour for the students..... thnk u so...much... now i m nt frustrated.......

[@shomusbiologyofficial]

Glad to hear that you're getting benefit from my lectures. Please subscribe and share

[@hayakhan1211]

thank u so much shomu. ur videos are a gem. i mastered blotting concept which i didnt even understood in med school. i wish u also had explained northwestern blotting. if u can then please also upload that

[@shomusbiologyofficial]

+haya khan thank you. Glad you liked my lectures

[@sugandhajha7740]

Before exams I assure to watch all your videos from my syllabus . 🙏

[@shomusbiologyofficial]

Glad to hear that you

[@risharajkhowa5060]

Sir ,you r just amazing at your teaching skill

[@shomusbiologyofficial]

Thank you

[@subhadipghosh9227]

Someone is there to teach you so easily.

[@sehar573]

Hi brother your method of delivering lecture really make me to fall for study 😁lots of love brother

[@shomusbiologyofficial]

Thank you so much for appreciating my efforts

[@rajlaxmiprasad7087]

Watched many times.helps lot.। Thank U sir

[@shomusbiologyofficial]

You're welcome

[@abithavarshinisivakumar7279]

I'm a microbiology student ur lectures helping out a lot

[@shomusbiologyofficial]

Glad to hear that you're getting benefit from my lectures

[@hurmajaved4869]

thanks alot sir ur videos are really helpful people all over the world are getting benefits from them including me.....i am from pakistan .GOD bless u sir

[@velushetty6709]

This video was very useful to my exam Thank you❤

[@shomusbiologyofficial]

You're welcome

[@madhuparnabannerjee8180]

My niper exams are in June and if I can make it through with biotechnology then all thanks to you coz I don't have to read books I just watch your videos and understand the concept. Thank you

[@hodannuuh1254]

He explained it very well , thanks a lot

[@shomusbiologyofficial]

You're welcome

[@aliraza-hc4nw]

bhai im from pakistan ur method off teaching isssss greaaat keep ittt up

[@shomusbiologyofficial]

Thank you. Glad you liked my lectures

[@mamtakol9575]

Thank you so much sir .ur videos are very useful for my study. U teach very well, i like so much ur ability to teach....

[@shomusbiologyofficial]

Thank you. Glad you liked my lectures

[@dipaliburle5733]

Nice shomus, u have very good commamd on all terms of life science, nice explanation

[@shomusbiologyofficial]

Thank you. Glad you liked my lectures

[@Heyitsanuradha]

I'm biotechnology student.. nd to be honest you teach much much better than my faculty.

[@shomusbiologyofficial]

Thank you so much for appreciating my efforts

[@mehulstar96]

Thank you so much!!! All your videos are so helpful!

[@shomusbiologyofficial]

+Mehul Patel thank you

[@physiologyfun1689]

Its amazing. ....huge respect from Pakistan

[@lahariramakrishna1868]

its really gud that u reply most of the ppl dropping a comment ,anyways thank u

[@shomusbiologyofficial]

You're welcome. Glad to hear that you're getting benefit from my lectures

[@sudipnale7664]

Sir your videos are really helpful for me.

[@shomusbiologyofficial]

You're welcome. Glad to hear that you're getting benefit from my lectures

[@vyghavinod2809]

Thank u sir..U r such a wonderful teacher

[@shomusbiologyofficial]

You're welcome. Glad you like it

[@obeddosty2938]

You're born to teach

[@shomusbiologyofficial]

Thank you so much for appreciating my efforts

[@sabasafura]

thank u...I wrote dis technique in my exam today...I was easily writing it...

[@ritikasoni3490]

amazing lecture sir. you explained it so easily to make it understand for us. 👍👍👍. very nice

[@shomusbiologyofficial]

Thank you. Glad you liked my lectures

[@pradeepsandaruwangunawardh8811]

very perfect and simply explained.. and thank you very much.

[@rohitasgaonkar8479]

I was confused in northern blotting thanks sir for explaining so precisely

[@fahmeedashaikh7793]

You explained everything very well. Thank you❤️

[@shomusbiologyofficial]

You're welcome

[@PriyaSingh-iz2qc]

U explained it in easiest way... 🙂🙂🙂

[@mayukhroy5393]

Your Video was too helpful, Sir.

[@shomusbiologyofficial]

You're welcome

[@helanpeter2429]

Sir, thankyou so much . It's so helpful.

[@shomusbiologyofficial]

You're welcome

[@amisharastogi9109]

Thankyou sir for such a nice explanation.

[@shomusbiologyofficial]

You're welcome

[@Zhi-u6j]

Great explanation thanks ❤

[@shomusbiologyofficial]

You're welcome

[@pascalineuwamariya605]

you did an amazing job explaining this!!!

[@jorgeribeiro9591]

You're amazing dude. Thanks a lot

[@shomusbiologyofficial]

+Jorge Ribeiro glad my lectures helped you out

[@kainatikhlaq4221]

beautiful method of teaching....thank you sir

[@shomusbiologyofficial]

You're welcome

[@shwetagupta9004]

You are genius suman sir .

[@shomusbiologyofficial]

Thank you so much

[@starnancy6173]

Hats off to your effort..Keep it up..

[@shomusbiologyofficial]

Thank you. Glad you liked my lectures

[@shrutikachawla2734]

Amazing lectures ,Thankyou so much Sir

[@shomusbiologyofficial]

You're welcome

[@aienchopz4648]

Thanks bro..it helps alot..got this particular topic presentation on monday 😄

[@ahangerruqiya5882]

Thanks sir aap bohut aache se explain karte ho.God bless u sir.

[@chakkravarthyv8333]

Please teach something apart from the book

[@shomusbiologyofficial]

That's called research. You need to do PhD for that

[@chakkravarthyv8333]

@@shomusbiologyofficial Dear Mr. Shomu, I think you have mistaken me. Your way of explaining the fundamentals are good and I really appreciate your efforts. I am just a mechanical engineer but I have some few suggestions for you, hope you don't take it wrong. If you are teaching about western blotting you can take a real time example. You can start like For eg: If a patient is undergoing hip transplant or bone transplant surgery then how will you find that the wound is getting healed or not? Lets see in what way Western Blot technique is going to be helpful. There are particular bone proteins ( RunX2, GAPDH, RANKL, Col-1) which can promote the bone joining and there are other sets of inflammatory proteins (TNF Alpha, TNF Beta, Interleukin 1,2) that are responsible for bone cell apoptosis and necrosis. So we need to find which are the proteins are upregulated and which are the proteins are down regulated. If bone joining proteins are upregulated then it means the patients bone joining ability is good , if inflammatory proteins are upregulated then wound healing process is hindered. To know either proteins are upregulated or downregulated we need to perform western blot analysis. After saying these things you shall explain all the fundamentals then all students can learn from the application perspective. I have one more kind suggestion for you. In SDS page in western blot is based on the molecular weight of the protein. Please give some examples. ( For an Example : The molecular weight of Col-1 is 138kDA, GAPDH is 36kDA). When we observe SDS page we shall find is there any bands that are present close to 138kDA or not . If a band is present but very less intense then it means that protein reaction is less intense. If it is a dark band then it means that particular protein has been upregulated. If there is no band in SDS page corresponding to 138kDA then that particular protein is absent. Finally we shall quantify the band intensity using Image J software which is a universally accepted software and free software. Like wise if you take a real time problem and if you explain in this way then it will quickly reach everyone and it will be more beneficial. In our Indian education we have read matrices, integral calculus during our school days and college days. But still now we never used any. Because we know how to do integral calculus but we don't know where to use it. So always teach from application perspective. A budding teacher like you should reach great heights. Good luck.

[@anagantisandhya644]

Thank u sir for ur classes

[@shomusbiologyofficial]

You're welcome

[@spmaheriya9935]

I'm your fan sir ! Awesome your all video's..👌

[@shomusbiologyofficial]

Thank you.

[@bongskitchen649]

thank sir very helpful video real i can understand each and very point

[@shomusbiologyofficial]

You're welcome. Glad to hear that you're getting benefit from my lectures

[@johntindell9591]

Thank you so much.

[@shomusbiologyofficial]

You're welcome

[@sidrakhan8589]

Sir you are doing a great job. Keep up good work

[@bassomalbarasy2214]

Thank you shomu 💗

[@shomusbiologyofficial]

You're welcome

[@angellesmith7585]

Amazing work!!! Thanks for sharing!!!

[@shomusbiologyofficial]

Glad to hear that you are getting benefit from the videos

[@faizanalam3789]

thank u so much its more helpful ,understand easily

[@shomusbiologyofficial]

+Faizan Alam you're welcome

[@amanialyaseer5129]

Ur like the best on youtube u rock

[@ameeshakushwaha9302]

Thank you so much sir

[@shomusbiologyofficial]

You're welcome

[@nehabhardwaj3483]

Waaooo sir amazing thankuuu so much sir

[@shomusbiologyofficial]

You're welcome

[@MSCstudent-gk1kz]

thanks my teacher

[@shomusbiologyofficial]

You're welcome

[@ssss...7896]

tq sr...its helpfull for my exam

[@nyashagava9476]

iwewe shomu mazivideo ako akarebesa aya shaaaa... apa unowanza ngano futi.

[@JyotiYadav-pz9ko]

Sir u r amazing

[@shomusbiologyofficial]

Thank you

[@markeshawtiruneh9591]

Thank u sir for your valuable videos

[@shomusbiologyofficial]

+Markeshaw Tiruneh you're welcome

[@markeshawtiruneh9591]

thank u Dear I proud of you would you tell me the specifications for Real Time PCR, DNA sequencer, Gell ellectrophoresis, DNA extaction kit, and then laboratory design since we are going to establish molecular laboratory and I am from Ethiopia

[@femina129]

Very impressive

[@shomusbiologyofficial]

Thank you

[@gayatribhatt4869]

Sir thanks a ton

[@shomusbiologyofficial]

You're welcome

[@tejasvisakhamuri1380]

Awesome!, thank you

[@shomusbiologyofficial]

You're welcome

[@meshackpeter9059]

I learned a lot from u'r lecture than what my lecturer toughted me!!

[@shomusbiologyofficial]

+Meshack Peter thank you. It's a great pleasure to know that my lectures are helping you.

[@carlostephenmoneva2528]

sir pls do check the complementary DNA sequence (DNA probe) in your example. Thanks your lectures are very informative and simple to understand

[@charlesmendith2444]

Thank you

[@shomusbiologyofficial]

You're welcome

[@sumantsahoo75]

Sir that buffer solution is SSC(sodium saline citrate)

[@akashnandy6802]

Shomu da.... Tumi guru shera

[@raibhangaikwad1818]

best videos sir . keep it up!

[@ujjwalchauhan.in.]

In 1977 , Northern Blotting technique was discovered not in 1979. Question . Is northern blotting used only for m-Rna ?

[@shomusbiologyofficial]

We need it for mRNA only

[@esthersharon366]

Very helpful.. loved the video...

[@afsajunaid3743]

what is the advantage of using aminobenzoxymethyl filter paper over nitrocellulose filter paper?

[@abuzarkhan527]

Southern techniques has been of enormous value but it was thought that it cud not be applied directly to the blot transfer of rna separated by gel electrophoresis since rna was found not to bind to nitrocellulose. Therefore james alwine david kemp amd george stark devised a procedure in which rna band are blot transferred from the gel on to the chemically reactive paper where they are bound covalently. The reactive paper is prepared by diazotization of aminobenzyloxy methyl paper.

[@oinambobochandsingh89]

Wonderful

[@shomusbiologyofficial]

Thank you

[@kalpanajaiswar5025]

Thank you so much sir 🤗

[@shomusbiologyofficial]

You're welcome

[@abumohammed9659]

WONDERFULL

[@shomusbiologyofficial]

Thank you

[@nidhinmangalasseri4061]

I have an question....that when we add dna probe how it hybridise with the targeted rna fragment... Bcz it consist of many uracil nitrogen bases... Can you please tell..... i understood all the blotting techniques all credits go to you

[@nayanavinayan7985]

Uracil can naturally pair with adenine through double hydrogen bonds just like thymine.thymine and uracil are similar expect that thymine has an additional methyl group

[@anjacerovsek6637]

The best! Thank you

[@shomusbiologyofficial]

You're welcome

[@satabdideka31]

Sir you mentioned the blotting buffer. Can you please tell what does the buffer contain?

[@keerthanab3294]

Thanks alot

[@shomusbiologyofficial]

You're welcome

[@manpreetsandhu2483]

Thank u sir 😊

[@shomusbiologyofficial]

You're welcome

[@PriyaSingh-iz2qc]

Thnq you.. 🙂🙂

[@jasmint7822]

some times board is not clear ,,,but ur amazing

[@Amruthasbiology]

Sir I have a doubt that why don't we use restriction enzymes to make the complex mRNA to linear RNA

[@abcdef-we5yk]

Very nice

[@aparnadevi1327]

Thank u sir. Sir I have a doubt that how can we add probe? Is there any process? Please clarify sir.

[@welcometosagnicksworld3894]

Sir can we transfer RNA from the gel into diazobenzylmethoxy paper or nylon paper electrophoretically ? Rather using capillary action ?

[@IshfaqAhmad-gb3qe]

thanks and love you sir

[@kelsey9336]

how northern blotting can easily distinguish between tissue specifically expressed genes and housekeeping genes

[@divyaswaroop9203]

Thanks 😊