Immunofluorescence

The lecture explains the principle of immunofluorescence technique and it also discuses the use of immunofluorescence in diagnosis.
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Immunofluorescence is a technique used for light microscopy with a fluorescence microscope and is used primarily on microbiological samples. This technique uses the specificity of antibodies to their antigen to target fluorescent dyes to specific biomolecule targets within a cell, and therefore allows visualisation of the distribution of the target molecule through the sample. Immunofluorescence is a widely used example of immunostaining and is a specific example of immunohistochemistry that makes use of fluorophores to visualise the location of the antibodies.
Immunofluorescence can be used on tissue sections, cultured cell lines, or individual cells, and may be used to analyse the distribution of proteins, glycans, and small biological and non-biological molecules. Immunofluoresence can be used in combination with other, non-antibody methods of fluorescent staining, for example, use of DAPI to label DNA. Several microscope designs can be used for analysis of immunofluorescence samples; the simplest is the epifluorescence microscope, and the confocal microscope is also widely used. Various super-resolution microscope designs that are capable of much higher resolution can also be used.

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In this immunofluroscenes test we can write RIA test bsc i think both are similar

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[@bharatacharaya1572]

Sir used compliment fixation test and immunofluorescence test results is same but why use different so main difference between this method.....?

[@IMMAb33]

Just a question, When is indirect used as opposed to direct?

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Thank you

[@soniyadas8972]

What is the advantage of secondary antibody in the immunofluorescense?

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[@firozsheikh3352]

nice video at 4:10 you says antibody attached to Wal then after some time you said antigen????

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Ag coated.

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[@harekrishnafamily1666]

hey shomu sir. you are doing a great job.. my question is in indirect immunofluorescence are we measuring the antigen conc or anybody conc ??? coz once you mentioned the patient sample containing antibody ll be measured and in he end you said we are measuring antigen.. plz clarify..

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[@shallavi]

Sir pls tell me that how we will come to know that which antibody is to be taken. ....as we dont know the type of antigen. ....pls pls pls sir clear my doubt

[@SShamimaNasrin]

You can find an antibody if you can figure out the source of your "unknown" antigen.

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[@Adnanafzal2022]

Then what is the difference between Elisa and Immunofluorescence?

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[@Crackheads305]

hey,is this a biochemical assay ?

[@mahmadchorahi]

Immunofluorescence is same term as of immunofluorescence light microscope

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Thank you. Its biostatistics by the way

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Thank. you. !!!!!!!!!!!!!!

[@Kaapa9]

i dont get Not all antiBodies connect to an AntiGen so how do you know which antibody you should put in the test if you dont know what kind of antiGens are there and thats what you are actually trying to find out

[@shallavi]

keez ape yes I also have same doubt

[@PreetKaur-fk3ds]

keez ape well these tests are perform when a medical practitioner is suspicious of some type of antigen, many tests are performed prior to this step to give a fairly good understanding of the disease / antigen.

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[@sajidmicrobiology]

study properly and then make videos .... 9:48 Antibodies present in the patients serum ?????? brother.. those primary antibodies are added externally they are not present in the serum... ant antigen antibody complex is not detected... only secondary antibodies are added after primary to increase the intensity of the signal... as per my knowledge ....

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