SYBR Green vs TaqMan - How qPCR works

[@josoborn6417]

Thanks for the great explanation ! I actually don't understand why you need the quencher in the Taqman technic. Wouldn't it work by just using the reporter and detect the emission when its separated by the polymerase ? Greetings from Germany !

[@INTEGRABiosciencesGlobal]

You need the quencher dye because you add the Taqman probes in excess to the reaction mixture. The quencher dye prevents that you detect a fluorescent signal from the probes that couldn't bind to a DNA sequence. Without the quencher dyes, all the reporter dyes would emit a signal and you couldn't distinguish between the probes that could bind to a DNA sequence of interest and the ones that couldn't. Quencher dyes absorb the fluorescent signal of reporter dyes when they are close together, which is the case when they are both attached to the TaqMan probe. Once the reporter dye gets displaced and cleaved by the polymerase, its signal can be detected because it moves away from the quencher dye. Let us know if you have additional questions and greetings from Switzerland!

[@josoborn6417]

@@INTEGRABiosciencesGlobal Thank you very much for the explanation ! That makes sense !

[@saravanamuralikrishnan7053]

Thank you

[@dikshanagvekar6938]

Please one video in reporter dye and quencher dye in detail

[@HodaMusavi-ep5og]

than you it was really comprehensive.

[@S.GUY-977]

thank you very much ❤✨ from SYRIA

[@laetitiaf.2671]

It was very clear thank you

[@INTEGRABiosciencesGlobal]

Glad it helped!

[@momenhamdy2260]

thx bro

[@aisyaalmaasmirantikartika1066]

Thank you so much, thats very clear👍🏻

[@INTEGRABiosciencesGlobal]

Thank you, we're glad it was helpful :)

[@LisaMartin-g7i]

Felicity Ridges

[@BarbaraGarcia-l3i]

Thomas Scott Jones Jeffrey Lee Nancy