The Complete Guide To Designing BioReactors | An Academics Insight

[@Jesus-kq4mp]

Attention: At 11:32 the X in exponential growth equations is not the same X in the biomass equation. In biomass equation, X is the total celullar mass in the bioreactor, e.g. , kg of biomass, while in exponential growth equation, X is the biomass concentration, e.g., kg/m3.

[@TheChemEngStudent]

Thank you for your feedback, I understand what you are referring to, however in this case the X term is indicative of biomass weight and not as a concentration. A similar empirical equation can be used to deduce the relative concentration, but in this case, it is simply the weight of biomass. But certainly, if you would like to discuss further, please do get in touch. Thanks again for watching, much appreciated!

[@lukedavies1978]

Great vitdoe Callum, was highly informative! Love to connect with you on LinkedIn

[@TheChemEngStudent]

Thank you for your kind words, glad you found it helpful!

[@jm-pi9rv]

Nice video. Thank you for making it. I have a question about the "Biomass yield from substrate = 0.52 g/g". When I use that I get a different number for cells: 1000L x 40 g/L = 40,000 g meth. 98% conversion means 39,200 g of meth consumed. With a biomass yield rate of 0.52 g/g we would get 20, 384 g of cells. Why the discrepancy? Thanks!

[@kylecatman7738]

4 min mark, is the bubble column closed?? Where's the outlet??

[@TheChemEngStudent]

Yes, the bubble column is a closed system, as are most bioreactors due to the cultivation duration. The schematics are just basic however, in more detailed ones you would find small relief vents, usually at the top to allow the air to escape, preventing an increase in pressure within the vessel. I hope this clears things up, and thanks for watching!

[@Neilkain-l3h]

So glad I found your channel!!

[@TheChemEngStudent]

Thank you for your kind words!

[@Tubbybeef]

Wow. Great video, very helpful. Do we find Umax by measuring the slope of the growth curve during the exponetial phase? If so, is this the same value we'd use for designing a continuous bioreactor using the same organism? For a given organism is Umax mostly independant of nutrient / substrate initial concentration? Does starting with more nutrient change the slope of the growth curve or does it just extend the duration of the exponential phase? Do you have any content related to continuous bio-reactors? Thank You!! Sub'd. :)

[@TheChemEngStudent]

Thank you for your kind words and for subscribing, much appreciated! Yes, Umax can be determined experimentally since you will monitor the growth periodically, you can deduce at which point the maximum growth rate occurs. Bioreactors typically don't run in continuous systems given there is a prolonged period required, unless the organisms are pre-cultivated beforehand to the design would be the same. Some variation occurs when using heterotrophic systems such as fermenters. This point really depends upon what variables/factors you intend on varying to establish if there are correlations between say nutrient concentration and growth rate. These would need to be carried out experimentally. Typically, from my experience different organisms will respond differently to nutrient starvation or surplus and so it is highly dependent upon the organism as well. I will be releasing more videos soon on other types of bioreactors so we can add in continuous systems for you certainly! I hope I have answered all your questions.

[@Adeballer-001]

Thanks

[@TheChemEngStudent]

Thanks for watching!